Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Isolation and Identification of Associated Bacteria and Maggots from Myiasis Affected Wounds of Cattle and Goats in Bangladesh

Md. Touhidul Islam
Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

Abdullah-Al-Maruf
Department of Livestock Services, Ministry of Fisheries and Livestock, Bangladesh Government, Bangladesh

Md. Abdul Mannan
Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh;

Hossain Md. Rakibur Rahman
Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

Md. Marofuzzaman Tarafder
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

Md. Abdus Samad
Department of Livestock Services, Ministry of Fisheries and Livestock, Bangladesh Government, Bangladesh

Abdullah Al Noman
Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh

Muhammad Belal Hossain
Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh

Md. Mizanur Rahman
Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

Abstract/ Executive Summary:

The study was carried out to investigate the associated maggots and bacteria in myiasis affected wound in animals. Total 37 myiasis affected cattle (n=25) and goats (n=12) were examined in this study, of which 21 samples were collected from the Veterinary clinics of Bangladesh Agricultural University (BAU), Mymensingh, and the remaining 16 samples were collected from Upazila Veterinary Hospital, Gouripur, Mymensingh. The maggots were collected from the wounds, and were identified under microscope. For microbial identification, samples (n=10) were collected aseptically by using sterile cotton swab. The samples were allowed to grow in media. Colony and staining characteristics were studied. Traumatic, creeping or migratory myiasis wounds were identified in the examined animals. In this study, subcutaneous wound was mostly recorded, of which 43% (n=9/21) was found at the Veterinary Clinics, BAU, and 44% (n=7/16) was found at the Upazila Veterinary Hospital, Gouripur. The maggots were identified as Chrysomya bezziana, commonly known as Old World screwworm. Occurrence of myiasis due to C. bezziana larvae was recorded as 100%. The associated bacteria were identified as Staphylococcus spp. In conclusion, the myiasis wounds are associated with both Staphylococcus spp. and larvae of C. bezziana. Preventive and control measures against the C. bezziana and Staphylococcus spp. can be undertaken to save the livestock from myiasis in Bangladesh.

Keywords: Bacteria, Control, Maggot, Myiasis, Wound type

Location: Veterinary Clinics of Bangladesh Agricultural University (BAU) and Upazila Veterinary Hospital, Gouripur, Mymensingh.

Start Date: 00-01-2012

End Date: 00-05-2012

Program Area: Animal Health and Management

Commodity: Goat, Cattle

Objectives:

The present research work was undertaken to investigate the wound types found in myiasis, identification and characterization of maggots causing myiasis in cattle and goat, and identification of bacteria infecting the myiasis wound.

Methodology:

Experimental animals: The research was conducted with the patients that were represented to two Veterinary Hospitals namely Veterinary Clinics of Bangladesh Agricultural University (BAU) and Upazila Veterinary Hospital, Gouripur, Mymensingh. The research was performed covey a period of five months starting from January 2012 to May 2012. Among the affected animals, 8 goats and 13 cattle were recorded in the Veterinary Clinics, BAU, Mymensingh. Similarly, patients admitted to the Upazila Veterinary Hospital, Gouripur, were taken into consideration for the research purpose. There were 10 cattle and 6 goats admitted to the Upazila Veterinary Hospital. The patients were investigated for wound type, maggot characteristics and categories of the secondary microbial invasions. Identification of maggots from wounds: The patient was restrained to examine the wound area. Gauze dipped in oil of turpentine or tincture of iodine was allowed to remain in the wound for 2 min. As a result, the maggots came out of the wound to the surface, and the maggots were removed with sterile forceps. The maggots were kept in plastic vials containing 70% alcohols. Then the vials were brought to the Department of Parasitology, BAU. The maggots were transferred from vials to petridish, and cleaned in 70% alcohol. The maggots were identified under microscope by observing body without fleshy processes and segments with belts of strongly developed spines, as described by Scholl et al. (2009). The length and width of the maggots were also measured. Identification of microorganisms from wound: Sources of samples: A total of 37 myiasis samples were collected, of which 21 were collected from Veterinary Clinics of BAU, and the remaining 16 were collected from Upazila Veterinary Hospital, Gouripur, Mymensingh, Bangladesh. Media used: A number of commercially available synthetic media were used for this study. All the media were of research grade. Chemicals and reagents: The chemicals and reagents used for the study were: reagents for Gram’s staining (Crystal violet, Gram’s iodine, acetone alcohol and Safranin), 3% hydrogen peroxide, normal saline solution, alcohol and other common laboratory chemicals and reagents of research grade. Collection and transportation of samples: The wound area was cleaned by using normal saline, and the samples were collected aseptically by using sterile cotton swab. Then the samples were brought to the laboratory with necessary precautions for bacteriological examination. Samples were disinfected with a swab soaked in methylated sprits. The samples were allowed to inoculate into Nutrient agar (NA) media and mannitol salt agar (MSA) media for the isolation and identification of bacteria. Nutrient borth (NB), NA, and MSA were prepared according to the manufacturer’s instructions. Isolation and identification of bacteria: Isolation of bacteria from the collected samples was done by inoculating the samples onto NA and MSA. The inoculated media were then incubated aerobically at 37°C for 24 h for growth. The isolates were identified based on their morphology and staining, cultural characteristics and biochemical properties. The representative bacterial colonies were characterized microscopically using Gram’s method (Islam et al., 2007; Begum et al., 2007; Nasrin et al., 2007; Roy et al., 2012; Asaduzzaman et al., 2013). Catalase test was done for differentiation of Staphylococcus sp. from other bacteria like Streptococcus sp.

Source of Information: J. Adv. Vet. Anim. Res., 2(2): 95-100, (June 2015)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The myiasis are associated with the larvae of Chrysomya bezziana. The microorganisms associated with myiasis is Staphyloccocus spp. The occurrence of myiasis wound is more frequent in the adult female cattle. Identification of the flies which are mostly responsible for myiasis in cattle and goats in Bangladesh are suggested.

Knowledge Management: Journal