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Determination of Minimum Nutrient for in Vitro Mycelial Growth and Preservation in Pure Culture

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Abdus Salam Khan
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Md. Mominul Hoque
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Taufiqur Rahman
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka

Abstract/ Executive Summary:

The experiment was conducted to evaluate the effect of nutrient on the mycelial growth rate of some commercially important oyster mushroom. The fastest mycelium growth rate and the minimum days required for completion of mycelium running in Petri plate were observed when Pleurotus djmour (POP₂ ) inoculated in PDA media at the ratio of 15 : 150 (Dextrose: Potato). Minimal mycelial growth rate and maximum days required for the completion of mycelium running were observed when Pleurotus djmour (POP₂ ) inoculated in PDA media at the ratio of 25 : 250 (Dextrose : Potato). No mycelial growth was observed when all variety inoculated in PDA media at the ratio of 0:0.

Keywords: Dextrose, Potato, PDA, Pleurotus ostreatus, Pleurotus djmour.

Location: Laboratory of National Mushroom Development and Extension Centre

Start Date: 00-08-2010

End Date: 00-09-2010

Program Area: Quality and Nutrition

Commodity: Mushroom

Objectives:

To determine the suitable concentration of potato & dextrose are used to prepare PDA media for the production of vigorous mycelium within short time.

Methodology:

This experiment was conducted in the tissue culture laboratory of National Mushroom Development and Extension Centre (NAMDEC) during August to September 2010. Two oyster mushroom species such as Pleurotus ostreatus (Po 2), Pleurotus djmour (Pop 1; Pop 2 , ) and different concentration of dextrose and Potato in Potato Dextrose Agar media (PDA), were used in this study. The inocula were collected from the germplasm centre of NAMDEC. Different amount of potato and dextrose were used for PDA media. The PDA media was prepared by 0g, 5g, 10g, 15g, 20g, 25g of dextrose and 0g, 50g, 100g, 150g, 200g, 250g, potato (According to treatment combination) mixed with 20g agar at pH 6.5. The mixture was boiled on gas burner until the agar dissolved. The media was poured into Petri plate at 20 ml/plates and sterilized in an autoclave for 20 minutes at 120°C under 1.5 kg/cm² pressure. After sterilization and solidification, the plates were inoculated with the inocula of testing mushroom species. Then plates were transferred in incubation room for mycelium running at 20-25°C temperature. The experiment was laid out in Completely Randomized Designs (CRD) with 4 replications. Data on mycelial growth rate and days to complete mycelium running, were collected and analyzed following standard methods using MSTAT-C computer Program. Means were computed following DMRT using the same computer program.

Source of Information: Bangladesh J. Mushroom. 5(2): 15-17, 2011 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

This study was to determine the suitable concentration of potato & dextrose are used to prepare PDA media for the production of vigorous mycelium within short time. The fastest mycelium growth rate and the minimum days required for completion of mycelium running in Petri plate were observed when Pleurotus djmour (POP2 ) inoculated in PDA media at the ratio of 15 : 150 (Dextrose: Potato). Minimal mycelial growth rate and maximum days required for the completion of mycelium running were observed when Pleurotus djmour (POP2 ) inoculated in PDA media at the ratio of 25 : 250 (Dextrose : Potato). No mycelial growth was observed when all variety inoculated in PDA media at the ratio of 0:0.

Knowledge Management: Journal