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Effect of Hot Water Extract of Calocybe Indica on Acute Metabolic Study

Mafruhi Sattar
Ethnopharmacology Laboratory, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

Alok Kumar Paul
Ethnopharmacology Laboratory, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

M. Reshma Khatun
Ethnopharmacology Laboratory, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

Paritosh Chakma
Ethnopharmacology Laboratory, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

Azizur Rahman
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

Calocybe indica, an edible mushroom is being used as a nutritious food supplement in Bangladesh. In this study effect of hot water extract of Calocybe indica (HWEC) on normal metabolic processes, acute metabolic study was carried out utilizing female Swiss-Webster mice. In the 24 hrs acute metabolic study, six parameters of normal metabolic process, i.e. water intake, urination, food intake, defecation, water content of the stool and weight of the dry stool were taken into account. Water intake was significantly (p<0.012) decreased at the 3rd hour after administration of the extract at the dose of 20 mg/kg body weight. And the weight of the dry stool though not significantly but yet was noticeably increased during the period of 9th to 12th hours with an overall decrease in the total period of the observation. Overall it can be concluded that the HWEC showed a mild decreasing effect on water intake, food intake and urination, with an insignificant increase in defecation and water content in stool during the period of 3rd to 12th hours after the administration.

Keywords: Calocybe indica, Hot water extract, Acute metabolic study

Location: Ethnopharmacology Laboratory, Department of Pharmacy, Jahangirnagar University, Savar, Dhaka

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mushroom

Objectives:

To study the elucidation of the metabolic study of Calocybe indica

Methodology:

Female mice (Swiss-Webster strain, 20-25gm body weight) bred in the animal house of the Department of Pharmacy, Jahangirnagar University, were used for the acute metabolic study. The mice were housed in plastic cages (having dimensions of 30×20×13 cm and bedding was soft wood chips) under controlled conditions of 12 hours dark-light cycles. They all received a basal diet of food preparation formulated by the Bangladesh Council of Scientific and Industrial Research (BCSIR) and tap water ad libitum. The mice were divided into two groups- Control and hot water extract of Calocybe indica (HWEC) treated group. Dried Milky mushrooms were collected from the National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh. The mushrooms were powdered using a grinder and preserved in air tight polythene packet. Ten g powder of Calocybe indica was mixed in 200 ml distilled water. The mixture was heated to reduce the volume. The condensed mixture was filtered off through a cotton cloth to get approximately 40 ml filtrated solution. Again 200 ml water was added with the residue and the total procedure was repeated to get 80 ml (40ml+40ml) filtrate, which was then heated and ultimately condensed to 40 ml. This liqui d is the water extract of the C. indica. The extract was administered orally at the dose of 20 ml/ kg body weight by using gastric gavage needles. Utilizing a ‘Nalgene Metabolic Case’ the effect of the extract (HWEC) on acute metabolism was performed. After a period of one day (i.e. 24 hours) of adjustment, they were administrated with graded dosage of the extract. The rate of food and water intake as well as defecation and urination were measured for as period of 3 days (72 hours) maintaining a 3 days of rest before each days (24 hours) of test. Eighteen mice were randomly selected and equally divided into 3 groups. These mice were placed in 3 different cases. The mice of one case were treated as control (administering distillated water) and the mice of the remaining 2 cases were treated with the same extract maintaining the same dose as duplicate. Within the next 24 hours, food and water intake as well as urination and defecation were measured with an interval of one hour for the first 4 hours, interval of two hours for the next 4 hours; next measurement was after another 4 hours and the final measurement after another 12 hours (i.e. a total of 24 hours). The test was repeated in the next two days with alternating control group (according to the Latin Square Design). Then the percent deviation of the drug treated group was compared with the corresponding control group. The results are expressed as mean±SEM (Standard error of mean). Means were compared by independent sample t-test. The statistical program “SPSS 12.0 for Windows” was used to test the level of significance. Probability (p) value of 0.05 or less (p<0.05) was considered as significant.

Source of Information: Bangladesh J. Mushroom, 4 (2): 27-34, 2010 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The hot water extract of Calocybe indica showed a mild decreasing effect on water intake, food intake and urination, with an insignificant increase in defecation and water content in stool during the period of 3rd to 12th hours after the administration. So, the mushroom might had no significant change in normal metabolic processes. So, Calocybe indica may be an ideal vegetable since it showed no constipating or laxative effect.

Knowledge Management: Journal