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Effect of Different Media, Ph and Temperature on Mycelial Growth and Substrates on Yield of Pleurotus Djamor

Tasnima Mahjabin
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

A. J. Kakon
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

K. M. Shamsuzzaman
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Md. Mominul Haque
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abdus Salam Khan
National Mushroom Development and Extension Centre, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

A study was undertaken to determine the optimum culture media, P^H , temperature and substrate on yield of pink oyster mushroom. The highest mycelial growth rate (0.24 cm) was observed in potato dextrose agar media and the lowest mycelium growth (0.11 cm) was observed in yeast media. The highest mycelial growth rate (0.41 cm) was observed in 25⁰C, whereas the lowest mycelial growth rate (0.20 cm) was observed in 30⁰C. Among the pH ranges the highest mycelial growth rate (0.45 cm) was observed in the p^H 6.5 and the lowest (0.11 cm) mycelial growth rate was observed in the p^H 4.5 which is statistically similar with p^H 9.0. Minimum days (13.25) required for completion of mycelial growth was observed in pasteurized rice straw substrate whereas the highest days (31.75) required for mycelial growth was observed in sugarcane baggase. The highest yield (253g) was obtained in paper substrate and the lowest yield (152.3 g) was shown by lentil + chickpea substrate. The highest biological efficiency (144.57%) was found in paper substrate and the lowest (87.03%) biological efficiency was found in lentil + chickpea substrate.

Keywords: Pleurotus djamor, Substrate, Yield, Biological efficiency.

Location: National Mushroom Development and Extension Centre, Savar, Dhaka

Start Date: 00-09-2010

End Date: 00-11-2010

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To determine the optimum culture media, P^H, temperature and substrate on yield of pink oyster mushroom.

Methodology:

The present study was carried out in National Mushroom Development and Extension Centre, Savar, Dhaka from September to November 2010. Pure culture was prepared on different media such as potato dextrose agar (PDA), malt extract agar (MEA), potato yeast dextrose agar (PDYA) and Yeast extract agar (YEA). Average mycelium growth and duration of complete mycelial growth was calculated. The selected medium was employed to evaluate the suitable temperature for mycelium growth of P. djamor. Same composition was adjusted at 6.5 p^H value before autoclave. After cooling the Petri plates were inoculated with the inoculums of the same mushroom. Then the inoculated plates were incubated at five different temperatures such as 10 ⁰C, 15 ⁰C, 20 ⁰C, 25 ⁰C, 30 ⁰C. PDA media was used as a base medium. Ten pH levels such as 4.5, 5.0, 5.5, 6.0, 6.5, 7.0,7.5, 8.0, 8.5 and 9.0. The media were evaluated for getting the best pH level for the pleurotus djamor mushroom cultivation. The p H levels were adjusted by adding 1N HCL or NaOH before autoclaving. The PDA medium was used as the base medium. Ten different substrates as, saw dust, sugarcane baggage, rice straw (hot water treated), news paper, coir, lentil husk, chickpea, lentil + chickpea, rice straw (autoclaved) and wheat straw. Spawn packets of all the substrates except the rice straw (hot water treated) were prepared, inoculated, and incubated following the procedure . The hot water treated rice straw was prepared. All the packets were 500 g. After completion of mycelium running, spawn packets were opened by “D” shaped cut on the shoulder and removed the polypropylene sheet then transferred to the culture house for fruiting body initiation. The relative humidity and temperature of the culture house were maintained at 80-90% and 20-25 ⁰C respectively by spraying water. The experiment was laid out in a completely randomized design (CRD) with four replications. Data was recorded and analyzed following standard methods using MSTATC computer program and means were computed following DMRT using the same computer program.

Source of Information: Bangladesh J. Mushroom. 5(2): 31-38, 2011 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest yield (253g) was obtained in paper substrate and the lowest yield (152.3 g) was shown by lentil + chickpea substrate. The highest biological efficiency (144.57%) was found in paper substrate and the lowest (87.03%) biological efficiency was found in lentil + chickpea substrate.

Knowledge Management: Journal