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Effect of Inoculum Levels of Fusarium Solani on the Development of Wilt in Potato

M.L. Rahman
District Commissioner Office, Tangail

M.A. Kashem
Plant Pathology Division, BINA, Mymensingh

M.M. Hossain
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

I. Hossain
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh

Abstract/ Executive Summary:

An investigation was carried out to evaluate the effect of different inoculum levels of Fusarium solani var. eumartii on the development of wilt diseases and plant growth of potato. Soil was inoculated by mixing oat grains colonized by the fungus at 0, 0.1, 0.5 and 1% (w/w) and its PDA culture at 0, 1, 5 and 10 blocks (10 mm)/pot. The highest level of inocula caused maximum reduction in plant growth and tuber weight, and the highest infection. Colonized oat grains at 1% and PDA culture at 10 blocks/pot reduced potato tuber weight by 69.22% and 40.06%, respectively.

Keywords: Potato, Inoculum levels, Fusarium solani

Location: Farm of Bangladesh Agricultural University

Start Date: 00-00-2010

End Date: 00-00-2010

Program Area: Pest Management

Commodity: Potato

Objectives:

1. To evaluate the effect of four levels of inoculum of F. solani. var. eumartii on wilt development,and growth and yield of potato.

Methodology:

Fusarium solani var. eumartii was isolated from diseased potato plants collected from experimental Farm of Bangladesh Agricultural University using potato dextrose agar (PDA) medium. It was purified by hyphal tip culture method and stored in PDA slants at 10±1ºC as stock culture. The soil of the investigation was sterilized with 2% formaldehyde and poured into earthen pots (30 cm x 25 cm). The isolate was grown on sterilized oat grains and PDA. Oat grains colonized by F. solani var. eumartii were air dried and used as inoculum. The colonized oat grains were mixed with soil in the pots maintaining inoculum levels of 0, 0.1, 0.5 and 1% (w/w). Blocks at 7 days old PDA culture of the fungus were cut with a 10 mm cork borer and used as inocula. PDA culture were mixed with pot soil at 0, 1, 5 and 10 blocks/pot. The design of experiment was completely randomized with 4 replications (pots). The seed tubers of cultivar Diamant, weighing 100g, were cut into two equal pieces and surface sterilized with 1% formalin solution by swabbing the tuber surface one day before planting. Seventy days after planting potato plants were removed from the pot and washed with tap water. Data on infection of stem, stolon and tuber; stem height, number and weight of stem, tuber and stolon were recorded. The data were analyzed statistically and means were compared following Duncan’s Multiple Range Test.

Source of Information: J. Agrofor. Environ. 5 (1): 37-39, 2011; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In both inoculation methods, the potato plants started wilting at 45 days of planting. Maximum wilting of plants observed at 55 days to 65 days. Severe wilting occurred at 1.0% inoculum level. Wilting started from the tip of the plant and became lin and thin and dull in compared to control.

Knowledge Management: Journal