Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Performance of Different Substrates on Growth and Yield of Shiitake Mushroom

Mohammad Golam Mohsin
Department of Agronomy and Agriculture Extension, Rajshahi University, Bangladesh.

Nirod Chandra Sarker
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Md. Aminul Hoque
Department of Agronomy and Agriculture Extension, Rajshahi University, Bangladesh.

Md. Bazlul Karim Choudhury
Ph.D. Student
Jahangirnagar University and OSD, DG Health, Mohakhali, Dhaka-1212, Bangladesh

Mahbuba Moonmoon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Tahera Binte Mujib
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

A. J. Kakon
National Mushroom Development and Extension Centre, Sobhanbag, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to determine the effect of different substrates containing sawdust of teak chambul (Michelia campaca), ipil-ipil (Leucaena leucocephala), teak (Tectona grandis), gamari (Gmelina arborea), rain tree (Albizia saman), mahagony (Swietenia mahagoni) and mango (Mangifera indica), mixed sawdust, mixed woodchips and rice straw on growth, yield and yield attributes of two strains (Le-8, Le-12) of shiitake mushroom (Lentinus edodes). Strain Le-8 showed the maximum mycelium growth rate (4.50 mm/day) and it required shortest duration of 23.75 days to complete mycelial running on mango sawdust. The strains required up to 82 days to complete mycelium running on other substrates. In Le-8, days to bump formation (DBF), days required from opening to harvest harvest (DOH) and total days for harvest (TDH) on different substrates ranged 90.0-120.8, 5.0-15.5 and 99.0-136.3 respectively. The Le-8 required lowest DBF on rain tree, DOH on mixed sawdust and TDH on gamari sawdust. In Le-12, the maximum mycelium growth rate of 3.20 mm/day and lowest duration (33 days) for mycelium running were found on gamari. The shortest days to harvesting (99 days) of fruit body was required by Le 8 on gamari sawdust. Number of fruiting body was also highest in Le-8 grown on mango sawdust. Yield attributes of two strains such as stalk diameter, pileus diameter and pileus thickness were significantly higher when culture on mango sawdust. The highest number of effective fruiting body, yield (189.8g/packet) and biological efficiency (108.4%) were recorded from the strain Le-8 grown on mixed sawdust followed by mango sawdust. In general, performance of Le-8 was better than Le-12 in terms yield and yield contributing characters. Strain Le-8, and mango and mixed sawdust may be recommended to grow shiitake mushroom under Bangladesh conditions. Considering yield and yield attributes, strain Le-8 and substrate prepared from mango sawdust and mixed sawdust may be recommended to grow shiitake mushroom in Bangladesh.

Keywords: Growth, Lentinus edodes, Ligno-cellulosic, Sawdust, Rice straw, Woodchips, Substrates.

Location: National Mushroom Development and Extension Centre, Savar, Dhaka

Start Date: 00-10-2011

End Date: 00-03-2012

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To evaluate influence of locally available substrates containing sawdust of different trees and agricultural waste on growth and yield of shiitake mushroom.

Methodology:

Eight substrates were prepared. Sawdust of teak chambul (Michelia campaca), ipil-ipil (Leucaena leucocephala), teak (Tectona grandis), gamari (Gmelina arborea), rain tree (Albizia saman), mahagony (Swietenia mahagoni), mango (Mangifera indica) trees and mixed sawdust were tested in the study as basic materials. The sawdust was supplemented with wheat bran, rice husk and CaCO₃ @ 27.27, 4.56 and 0.2% (w/w). Two additional substrates containing mixed wood chips and rice straw were included in the experiment without any supplement. So, there were ten treatments in the experiment. The rice straw were chopped into 4-5 cm long and soaked in warm water at 60 ^oC for one hour. After soaking excess water was drained off and the straw was air dried to fix the moisture content at 65%. To estimate the moisture content, air dried straw pieces were pressed between palms. If no water drop was released from the straw the moisture content was considered to be appropriate. Woodchips were cut in small pieces (4-5 cm) and soaked in water overnight. Then drained out the water, was used without any supplementation. The experiment was carried out at a mushroom culture house of the National Mushroom Development and Extension Centre, Savar, Dhaka, during the period from October 2011 to March 2012. Two strains of shiitake mushroom (Lentinus edodes), namely Le-8 and Le-12 were used as test materials. To prepare mother culture of the test mushroom sawdust and wheat bran were mixed together at 2:1 (v/v) and supplemented with CaCO₃ at 0.2% (w/w) of the mixture. The moisture level of the mixed substrate was maintained at 65% with tap water. The substrate was poured into polypropylene bags (18 cm× 25 cm) at 300 g/bag. The substrate mixture was poured into 18 cm × 25 cm polypropylene bags at 500 g/bag. The neck of the bag was prepared by using heat resistant plastic pipe. A hole of about 2/3 deep of the bag was made for space to introduce the inocula. The neck of each poly bags was plugged with cotton, covered with brown paper and tied with a rubber band. The packets were sterilized in an autoclave for 2 h at 121 ^oC under 1.1 kg/cm 2 pressures. Both the strains showed optimal mycelial growth at 22 ± 2 ^oC and 60-70% relative humidity in the culture house. After full colonization of the spawn packets, a thick mycelial coat formed on the outer surface of colonized substrate. Temperature, relative humidity and light intensity of the culture house were maintained at 18-22 ^oC, 60-70 % and 10-20 lux, respectively. Sufficient water was sprayed every day and proper aeration was maintained in culture house for the release excess CO₂ and supply of sufficient O₂ as required for the development of primordia and fruiting bodies. The packets were arranged in culture house following completely randomized design with 4 replications. Data on mycelium growth rate, days to complete required mycelium running, bump formation, days to first harvest, and last harvest from opening, number of fruiting body and effective fruiting body, length and diameter of stalk, diameter and thickness of pileus, yield (g/packet) and biological efficiency were recorded.

Source of Information: Bangladesh J. Mushroom. 6(2): 9-18, 2012 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Based on findings of the study it may be concluded performance of Le-8 was better than Le-12 in terms of growth rated, mushroom yield and biological efficiency of substrate. Le-8 gave highest yield on mango sawdust followed mixed sawdust. Considering yield and yield attributes, strain Le-8 and substrate prepared from mango sawdust and mixed sawdust may be recommended to grow shiitake mushroom in Bangladesh.

Knowledge Management: Journal