A. A. M. Bhuyan
Departments of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M. M. Rahman
Departments of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M. A. Hashim
Departments of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
M. K. Islam
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
M. N. Islam
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
A. Islam
Departments of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
Role, Omentum, Wound healing, Goats
Departments of Surgery and Obstetrics
Animal Health and Management
Experimental animals - A total of nine goats (Capra hircus), three male and six female, aged 8-10 months, weighing 7-8 kg were used. Management of animals - The experimental animals were purchased from the local market. The animals were kept on tethered grazing and supplied with 500 gm bran and concentrate mixture in two meals and drinking water ad libitum. The goats were dewormed with albendazol (Almex®, Square Pharmaceuticals Ltd, Bangladesh) @ 7.5 mgkg-1. Experimental design - The goats were randomly divided into three groups, A, B and C. In group A, only omentum flaps were used. The flaps of omentum were trimmed to 2 cm long by 0.4 cm. The omentum was put into the depth of the wound and sutured. In group B, omental flaps were used in combination with antibiotic. Procaine penicillin with streptomycin sulphate (Streptopen®, Reneta Animal Health Ltd, New DOHS, Mohakhali, Dhaka) was injected daily for 5 days intramuscularly at 15 mgkg-1. In group C, neither omental flap nor antibiotic was administered, only normal saline (0.9% NaCl) was used to six wounds produced in three animals. The wounds were sutured with nylon thread by the pattern of simple interrupted and the group was kept as control. Wounds were cleaned daily with distilled water with the help of sterile gauze. Animals were maintained carefully to avoid contamination or infection. Movement of animals was restricted to prevent granulation tissue formation. Source of omentum - Goat’s omentum was collected immediately after slaughtering from the local abattoir and kept in normal saline. Omentum was sliced as a flap of 2 cm by 0.4 cm with the help of scalpel and tissue forceps on sterile Petri dishes. The flap was used in the wound within one hour of collection. Antibiotics used - Procaine penicillin with streptomycin sulphate (Streptopen®, Renata Animal Health, New DOHS, Mohakhali, Dhaka, Bangladesh) was used to counter bacterial infection. Preparation of the wound - The operation sites were cleaned and painted with ethyl alcohol, and infiltrated with 2% lignocaine hydrochloride (Jasocaine®, Jayson Pharmaceuticals Ltd., Dhaka, Bangladesh). On day 0, two incisions, each 2.5 cm long and 0.5 cm deep were made on either side of the midline of the lumber region. Gross observations - The condition of wounds was monitored and the progress of healing recorded. The duration of wound healing, exudation, haemorrhage, oedema, inflammation, stitch abscess, rupture of stitch were recorded up to complete healing. A wound was assumed to be healed when cicatrisation and pigmentation were found. Evidence of pain was ascertained by palpation of the wound and its area. Biopsy and Histopathology - The biopsies were collected from the wound areas of each experimental animal on day 3, 5, 7, 10 and 15 with standard surgical procedure. About 1.5 x 1.5 cm of tissues was collected and the samples containing dermis and epidermis were kept in 10% buffered neutral formalin for 9 days. The tissues were trimmed and fixed for 24 hours in buffered formalin. Tissues were kept in running tap water overnight to wash out formalin and dehydrated in 50%, 70%, 80% and 95% alcohol and three changes of absolute alcohol for one hour each. The sections were cleaned in chloroform by two changes, one and half an hour for each. Tissues were embedded in paraffin, sectioned (5-μm thick), stained with Haematoxylin and Eosin, and examined under light microscope. Statistical analysis - The data were statistically analyzed by Complete Randomized Design (CRD) one-factor test.
Bangl. vet. 2008. Vol. 25, No. 1, 1-8
Journal