Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Different Levels of Nitrogen and Sulphur with or Without Biofertilizer on Nodulation and Plant Growth of Mungbean

A.T.M.R.A. Chowdhury
Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh

M.M.K. Khan
Bangladesh Institute of Nuclear Agriculture, Mymensingh

M.S. Islam
Department of Environmental Science, Bangladesh Agricultural University, Mymensingh

H. Mahmud
Department of Agricultural Extension, Khamarbari, Farmgate, Dhaka.

B.K. Biswas
Department of Agricultural Extension, Khamarbari, Farmgate, Dhaka.

Abstract/ Executive Summary:

A field experiment was conducted at Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, during the period from February/2008 to April/2009 to evaluate the effect of N and S fertilizers with or without bio-fertilizer (Bradyrhizobium) on nodulation and plant growth of Binamoog-6. The experiment was laid out in Randomized Complete Block Design (RCBD) with nine treatments and three replications. The treatments were T₁-N₀S₀, T₂-N₀S₅, T₃-N₀S₁₀, T₄-N₁₅S₀, T₅-N₁₅S₅, T₆-N₁₅S₁₀, T₇-N₃₀S₀, T₈-N₃₀S₅ and T₉-N₃₀S₁₀ kg ha^-1 with or without bio-fertilizer (Bradyrhizobium). Data were recorded at 35 days after sowing (DAS), 49DAS and at harvest. There were significant variations among the different treatments combination in terms of nodulation and plant growth. Result showed that Bradyrhizobium inoculation significantly increased the number of nodules plant^-1, nodule dry weight plant^-1 and plant dry weight. The highest nodule number plant^-1 (62.13 at 49 DAS), nodule dry weight plant^-1 (115.40mg at 49 DAS) and plant dry weight (15.08gm at harvest) were recorded in T₃-N₀S₁₀ treatment. The result revealed that the inoculation of bio-fertilizer (Bradyrhizobium) significantly increased the plant growth and nodulation of Binamoog-6.

Keywords: Biofertilizer, nodulation, plant growth

Location: Bangladesh Institute of Nuclear Agriculture (BINA) Farm, Mymensingh

Start Date: 00-02-2008

End Date: 00-04-2008

Program Area: Crop-Soil-Water Management

Commodity: Mungbean

Objectives:

1. To evaluate the effect of Bradyrhizobium inoculation with nitrogenous and sulphur fertilizers on the nodulation and plant growth of summer mungbean.

Methodology:

The experiment was conducted at the Bangladesh Institute of Nuclear Agriculture (BINA) Farm, Mymensingh during the period from February to April 2008-2009. The experimental area was characterized by high temperature and heavy rainfall during the kharif season (March-September) and low rainfall and moderately low temperature during rabi season (October-February) to evaluate the effect of N and S fertilizers with or without bio-fertilizer (Bradyrhizobium) on nodulation and plant growth of summer mungbean (Vigna radiata L.) cv. Binamoog-6. The experiment was laid out in Randomized Complete Block Design (RCBD) with nine treatments and three replications. The treatments were T₁-N₀S₀, T₂-N₀S₅, T₃-N₀S₁₀, T₄-N₁₅S₀, T₅-N₁₅S₅, T₆-N₁₅S₁₀, T₇-N₃₀S₀, T₈-N₃₀S₅ and T₉-N₃₀S₁₀ kg ha^-1 with or without bio-fertilizer (Bradyrhizobium) having a size of each plot was 3 x 2m² with a distance between two adjacent blocks were 0.5m and between plots 0.40m ails were prepared. The initial soil sample was analyzed for texture, PH, organic carbon, total nitrogen, available phosphorous by using the appropriate methodology. A basal dose of K fertilizer at the rate of 35 kg K ha-1 as muriate of potash (MoP), phosphorus fertilizer at the rate of 20 kg P ha-1 as triple super phosphate (TSP), sulphur fertilizer as gypsum and nitrogen fertilizer as half urea was applied at final land preparation. The rest of half urea was applied 25 days after sowing (DAS). The fertilizers were then mixed well with the soil by spading and individual unit plots were leveled. The Bradyrhizobial inoculants BINA T301 and BINA 301were prepared in Soil Microbiology and Bio-fertilizer Laboratory, BINA, Mymensingh. Viability count of rhizobia in the yeast extract mannitol broth media was made on the day before use the plate count method. Before inoculant mixed with seeds and made black with each seed, molasses was added and mixed thoroughly with seed so that all the seed coats became sticky. Seeds were sown into the furrow maintaining a line to line distance of 40 cm and the furrows were covered by soils soon after seeding. Two wedding and thinning were done at 8-10cm and 22-25cm of plant height maintaining a plant to plant distance was 8 cm. Control of insect and pathogen and drainage of rain water was done as and when necessary. Data were recorded at 35 days after sowing (DAS), 49 DAS and at harvest. From each plot, 5 randomly selected plants were carefully uprooted with the help of a nirani so that no nodule was left in the soil. The nodules from main and branch root of each plant were collected and counted after carefully washed in water. Finally plant and nodule fresh weight and dry weight were recorded. All pods of mungbean was not matured at a time so, the crop was harvested in two times. The pod and straw from every plot was harvested separately. From each plot 5 randomly selected plants were collected at harvesting period and then plants of 2.5 sq. m. were harvested and tied with rope separately. Data on number of pods and grains plant^-1, 1000-grain weight were recorded from 10 plants. After 4 days sun drying processed grain and straw were again dried in the sun for 3 days. Grain and straw yields were recorded plot wise and converted in to kg ha^-1. The analysis of variance for various characters of mungbean and nutrient content was done following the F-statistics. The mean comparison of the treatments was made by Duncan’s Multiple Range Test (DMRT).

Source of Information: J. Agrofor. Environ. 5 (1): 59-62, 2011; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that Binamoog-6 significantly responded to combined use of different levels of nitrogen and sulphur fertilizers with or without bio-fertilizer. Inoculation of Bradyrhizobium significantly increased the nodulation and plant growth of Binamoog-6. However, further trials may be conducted at different agro-ecological zones to decide the exact dose of nitrogen and sulphur fertilizers with bio-fertilizer in a specific area of Bangladesh.

Knowledge Management: Journal