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Introgression of Submergence Tolerance Qtl, Sub1 Into BRRI Dhan44 Through Marker Assisted Backcross Breeding

Dr. Md Enamul Hoque
CSO (A.C.)
Biotechnology Division Bangladesh Rice Research Institute (BRRI) Gazipur-1701.

Dr. Jannatul Ferdous
Senioe Scientific Officer
Biotechnology Division Bangladesh Rice Research Institute (BRRI) Gazipur-1701.

Dr. KM Iftekharuddaula
Principal Scientific Officer
Plant Breeding Division Bangladesh Rice Research Institute (BRRI) Gazipur-1701.

Abstract/ Executive Summary:

To make the rice variety BRRI dhan44 submergence tolerant for about two weeks through marker assisted backcross breeding, BRRI dhan52 was used as the donor parent for sub1 gene and the BRRI dhan44 was used as the recurrent parent. Both parent were crossed to generate F₁ population and then backcrossed. Primer survey was done between parents for selecting the polymorphic markers. Foreground selection was done in the BC₁F₁,BC₂F₁ and BC₃F₁population for selecting the plants possessing the target QTL. Also polymorphic markers were used in BC₃F₁ and BC₄F₁ generation for background selection of BRRI dhan44. Five homozygous plants of BC₄F₁were selected for backcrossing. Foreground selection was done in BC₅F₁generation to confirm the plants having SUB1 QTL. In BC₅F₂plants, foreground selection was done again with SUB1C173 primer to confirm homozygous plants for SUB1 QTL in BRRI dhan44. Twenty-six homozygous plants for SUB1 QTL were grown as observational trial during Boro 2013-14 season. Among them 13 lines were selected for further evaluation as preliminary yield trial (PYT) and screened under submergence condition. These lines with submergence tolerance might be selected as a variety for the southern part of Bangladesh.

Keywords: BRRI dhan44, Sub1 QTL, MAS, Submergence tolerance

Location: Biotechnology Division, BRRI HQ

Start Date: 01-07-2011

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To make the variety BRRI dhan44 submergence tolerant for about two weeks.

Methodology:

BRRI dhan52 was used as donor parent for SUB1 gene and the BRRI dhan44 was used as recurrent parent. Both parents were crossed to generate F₁ population and then backcrossed. SSR primer survey was done to select the polymorphic markers which are distributed throughout the rice genome. This data were used for background selection afterwards. Foreground selection was done in the BC₁F₁and BC₂F₁ populations to select the plants possessing the target gene. Best plants within the backcross populations were selected phenotypically. In this way BC₃F₁ population was generated and then background selection was done. On the basis of background selection, five homozygous plants of BC₄F₁were selected for further backcrossing. Foreground selection was done in BC₄F₁and BC₅F₁generations and heterozygous plants were selected with SUB1C173 primer. In BC₅F₂generation, foreground selection was done again with SUB1C173 primer to confirm SUB1 QTL in BRRI dhan44.

Source of Information: BRRI Annual Report 2013-14, page no.-16

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Thirteen promising advanced breeding lines having Sub1 QTLs were identified.

Knowledge Management: Report/Proceedings