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Studies on the Biodiversity of Aseptate Gregarine Parasite from the Oligochaete Host of Bangladesh

Sutapa Sarkar
Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani- 741235, West Bengal, India

Probir K. Bandyopadhyay*
Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani- 741235, West Bengal, India

Abstract/ Executive Summary:

Biodiversity studies in search of endoparasitic acephaline gregarines of the earthworms of Dhaka, Bangladesh, revealed a new species under the genus Nematocystis Hesse, 1909. The species was obtained from the seminal vesicles of the earthworm Metaphire peguana Rasa, 1890. It is a perfectly ribbon like organism, solitary, cylindrical, with seve ral bulges and foldings in the middle portion of the body, especially in mature forms. Mature gamont measures 1955.85-1989.0 (1970.215 ± 13.830) µm in length and 48.62-53.04 (51.714 ± 2.135) µm in width. Nucleus small and rounded, measures 26.52-33.15 (29.835 ± 2.992) µm in length and 17.68-22.01 (19.448 ± 2.282) µm in width. Gametocysts spherical with two approximately equal sized gametocytes. It measures 110.05-121.55 (116.24 ± 4.908) µm in length and 88.04-99.45 (93.925 ± 5.823) µm in width. Oocysts biconical, measuring 22.1-30.94 (26.962 ± 3.870) µm × 11.05-15.47 (13.26 ± 2.08) µm.

Keywords: Biodiversity, Aseptate gregarine parasite, Oligochaete host

Location: Dhaka, Bangladesh

Start Date: 00-04-2011

End Date: 00-06-2011

Program Area: Conservation and Biodiversity

Non-commodity: Aquatic animal

Objectives:

To describe a new species of aseptate gregarine from the oligochaete host, Bangladesh.

Methodology:

The biotic survey of gregarine parasites in oligochaetes was performed in Dhaka, Bangladesh. Samplings were carried out in search of earthworm hosts in Dhaka, Bangladesh. The host earthworms were collected during the months of April -June 2011 from the drainage soil and the collected earthworms were keptin soil in a tub and taken to the laboratory alive. Some of the earthworms were dissected while alive and their seminal vesicles were carefully removed. These were placed on clean glass slide with a drop of 0·6% NaCl solution. A thin film of seminal fluid was drawn out on a slide covered with a cover slip for the examination of living protozoan under a light microscope. After initial study of living protozoans, the content of the seminal vesicles was semidried and fixed in Schaudin’s fluid for 20 minutes. The smears were stored in 70% ethanol for the removal of mercuric chloride. The slides were then passed through a descending series of alcohol (five minutes each) and placed in distilled water. These were transferred to a 3% Iron Alum solution (Over night) and stained with Heidenhain’s hematoxylin for 20 minutes. Differentiation was done with 1% iron alum solution under the low power objective lens of the light microscope. The slides were then washed thoroughly, dehydrated in an ascending series of alcohol, cleared in xyline and mounted in DPX. All measurements were made with a calibrated ocular micrometer. In each case minimum and maximum values are given, followed in parentheses by arithmetic mean, standard deviation. For statistical analysis measurements of 20 specimens were taken into account. Photomicrographs were taken using an Olympus light microscope (model CH-2) and Olympus digital camera (model CX41). The methods of describing the shapes of planes and solids have been done following Clopton.

Source of Information: Bangladesh J. Zool. 40(1): 51-58, 2012

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The biodiversity study of protozoan parasites from oligochaete hosts of Bangladesh revealed that the seminal vesicle of Mataphire peguana are infected with a new species of the genus Nematocystis and while compairing the other species described earlier, it seems to be new to science and a new name has been proposed in this communication.

Knowledge Management: Journal