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In Vitro Regeneration of Anthurium Andreanum Cv. Nitta

S. A. ISLAM
Scientific Officer
Bangladesh Rice Research Institute (BRRI), Gazipur

M. M. R. DEWAN
Scientific Officer,
Bangladesh Rice Research Institute (BRRI), Gazipur

M. H. R. MUKUL
Scientific Officer
Bangladesh Rice Research Institute (BRRI), Gazipur

M. A. HOSSEN
Scientific Officer
Bangladesh Rice Research Institute (BRRI), Gazipur

F. KHATUN
Department of Agricultural Extention Education, Bangladesh Agricultural University (BAU), Mymensingh

Abstract/ Executive Summary:

The present study was undertaken to investigate the effect of different combinations of NAA, IBA, and BAP on in vitro organogenesis of Anthurium andreanum cv. “Nitta” at the DEBTEC (Development of Biotechnology & Environmental Conservation Centre) Laboratory, Dhaka. Organogenesis from leaf mid rib to shoot initiation required 9.00 days and the best survivality was 80.00% percent at 30 DAI (Day after initiation) with the combination of 1 mg/L NAA and 1 mg/L BAP in MS media. Among the 20 hormone supplements, MS media containing 1 mg/L NAA and 1 mg/L BAP showed the highest shoot formation (68.30%), number of shoots/explant (3.37) and the longest shoot (4.65 cm) at 60 DAI. MS media without any hormone (control) showed the poorest performance in regeneration of shoots. On the other hand, MS media containing 1 mg/L IBA + 1 mg/L BAP showed the best performance in rooting of shoots (83.85%), highest number of roots (4.29/plantlet), root elongation (5.50 cm) were recorded at 60 DAI.

Keywords: In vitro regeneration, Anthurium andreanum

Location: Dhaka

Start Date: 01-12-2004

End Date: 30-04-2005

Program Area: Variety and Species

Commodity:

Objectives:

This study was undertaken to standardize the media with NAA and BAP for rapid multiplication of Anthurium andreanum cv. ‘Nitta’ through organogenesis from the mid rib of young leaf (red colour) and to regenerate shoot and root was multiplied by the media with IBA and BAP.

Methodology:

The present study was carried out at the DEBTEC (Development of Biotechnology & Environmental Conservation Centre) Laboratory, during December, 2004 to April, 2005. Anthurium andreanum cv. Nitta (red colour) was collected from the DEBTEC Nursery; project site: South Panishile, Gazipur. The mid ribs of young leaves were used as explants to study the shoot regeneration, shoot multiplication and rooting in MS medium supplemented with following hormones For determination of survivality and development of organogenesis of leaf mid rib in MS (Murashige & Skoog, 1962) medium was used with hormone combination NAA @ 0.5, 1 and 1.5 mg/L each with BAP @ 0.5, 1, 1.5 and 2 mg/L, respectively. For shoot differentiation, hormone combinations of NAA @ 0.5, 1 and 1.5 mg/L each with BAP @ 0.5, 1, 1.5 and 2 mg/L used in MS media for shoot multiplication. For root induction, different combinations of IBA @ 0.5, 1 and 1.5 mg/L and BAP @ 0.5, 1, 1.5 and 2 mg/L used in MS media for rooting of regenerated shoots of Anthurium andreanum. The unfold leaf segment were collected and washed thoroughly under running tap water and dead tissues were carefully removed with the help of asharp stainless steel knife. The leaf segment was chopped into small pieces and sterilized by 70% ethanol for 30 seconds then washed 3 times with double distilled water. Afterwards, it was sterilized with 0.1% Mercuric chloride (HgCl₂) solution for 10 minutes and washed several times then inoculated into vials containing 25 ml MS media with different hormone concentrations. Each treatment was conducted with four replications. The culture medium, glass wares and instruments were sterilized by autoclaved at 15-psi pressure at 121°C for 20 minutes and the culture room was surface disinfected with 70% ethyl alcohol to ensure aseptic condition under in vitro propagation. Cultures were maintained in an incubator at 25°C temperature in complete dark condition for organogenesis. The organogenesis placed on MS medium supplemented with NAA and BAP for shoot regeneration was maintained within 25±2°C by air conditioner at 16 hours light period by the illumination from white florescent tube light (Phillips). When the shoots grew about 3-4 cm in length with 4-5 well developed leaves they were rescued aseptically from the culture tubes and were separated from each other and again cultured on freshly prepared MS medium containing different concentrations of IBA and BAP for root induction. The effect of different treatments on survivability (%), days required for organogenesis, percent of shoot induction, shoots per explant, shoot length (cm), percent of root development, roots per plantlet and root length were collected and statistically analyzed. The experiment was conducted in growth room and arranged in completely randomized design (CRD). The analysis of variances for different characters was performed and the means were compared by least significant difference (LSD) test for interpretation of results (Gomez and Gomez, 1984).

Source of Information: Bangladesh J. Agril. Res. 35(2) : 217-226, June 2010 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Mid rib of Anthurium andreanum cv. Nitta were cultured on MS medium supplemented with I mg/L NAA + I mg/L BAP showed the best result for percent survivability (80.00%). Shoot initiation in MS media containing 1 mg/L NAA +1 mg/L BAP took minimum time of 9.00 days, maximum percentage of shoots induced explant was 68.3 0%, the number of shoots per explant were 3.37 and the length of shoots was highest (4.65 cm) at 60 DAI and similar result was shown at 40 DAI and 20 DAI, respectively. MS media containing 1 mg/L IBA + 1 mg/L BAP showed the best performance in rooting of shoots (83.85%), highest number of roots (4.29/plantlet) and root elongation (5.50 cm) at 60 DAI and same trend was found at 40 DAI and 20 DAI, respectively. Shoot regeneration and rooting was poor on MS media without any hormone supplement. The protocol developed from the experiment may be useful for large-scale production of healthy and disease free planting materials of Anthurium andreanum cv. Nitta commercially. Also these findings of the study may be used for genetic transformation for the improvement of Anthurium andreanurn cv. Nitta using biotechnological approach.

Knowledge Management: Journal