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Effect of Cutting Materials on Regeneration and Development of New Shoots of Jiga (garuga Pinnata)

M.H.A. Khanam
A H Z Biotech. Ltd, Padma Residential Area, Vadra, Rajshahi, Bangladesh

M.A. Hossain
Department of Agroforestry, Bangladesh, Mymensingh

M.O. Islam
Department of Crop Botany, Bangladesh, Mymensingh

M.N.A. Chowdhury
Spices Research Center, BARI, Shibganj, Bogra, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted to study the regeneration and shoots development on cutting materials of Jiga (Garuga pinnata) at the Agroforestry Farm, Department of Agroforestry, Bangladesh Agricultural University, Mymensingh, during the period from March to September, 2004. The experiment was laid out in Randomized Complete Block Design with three replications. The treatments were three different cutting lengths viz. 50, 25 and 12.5 cm and thickness viz thick stem cuttings of 2.75 cm diameter ranging from 2.5 to 3.0 cm in thickness and thin stem cuttings of 1.5 cm diameter ranging from 1.0 to 2.0 cm in thickness. It was found that regeneration and shoot development leading to forage production, the new bud development was first observed by day 7 and the peak increase in bud number was observed on 21 DAP in 25 cm × thick stem treatment. The treatment of 50 cm × thick stem showed sharp increase in the development of branch number at 90 DAP proving it to be the most effective treatment. The results of comparative study showed that with the increase in number of branches, the number of buds decreased. However, branch development was comparatively slower than that of buds. The length of branch was found increased gradually with the increase in DAP, and it was significantly higher in the longer cuttings than that in shorter ones. Similarity The number of leaf was found to increase gradually with the increase in DAPs and it was significantly higher in longer cutting than that in shorter ones. The interaction effect on the increase in number of leaves also showed almost similar trend as that of number and length of branches.

Keywords: Cutting length, Thickness, Regeneration

Location: Agroforestry Farm, Department of Agroforestry, Bangladesh Agricultural University, Mymensingh

Start Date: 00-03-2004

End Date: 00-09-2004

Program Area: Crop-Soil-Water Management

Commodity: Tree

Objectives:

1. To study the regeneration and shoots development on cutting materials of Jiga (Garuga pinnata).

Methodology:

The experiment was conducted to study the regeneration and shoot development on cutting materials of Jiga (Garuga pinnata) at the Agroforestry Farm, Department of Agroforestry situated on the central area of agricultural farm of Bangladesh Agricultural University, Mymensingh, during the period from March to September, 2004. The experimental area is located (AEZ-9) at 24.75°N latitude and 90.50°E longitude at a height of 18 m above the sea level. Garuga pinnata Roxb. was used for the present study. The experiment was laid out in Randomized Complete Block Design with three replications. The treatments were three different cutting lengths (50, 25 and 12.5 cm) and thickness (thick stem cuttings of 2.75 cm diameter ranging from 2.5 to 3.0 cm in thickness and thin stem cuttings of 1.5 cm diameter ranging from 1.0 to 2.0 cm in thickness). Well decomposed cowdung @ 16 kg/plot of 3.5 m × 4.5 m size were used by mixing to soil as basal dose during final spading and leveling before plantation for the experiment. No chemical fertilizer was used in plot prior to plantation. However, only urea @ 211.64 kg/ha were applied topdressing at mid stage of the study. The recorded parameters were number of buds and branches. The recorded data were analysed statistically to find out variation resulting from experimental treatments using MSTAT package programme. The mean for all treatments was calculated and analysis of variance under study was performed by F-variance test at 5% level of significance. Duncan’s Multiple Range Test (DMRT) separated the means of the parameters.

Source of Information: J. Agrofor. Environ. 5 (1): 135-138, 2011; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It was found that regeneration and shoot development leading to forage production, the new bud development was first observed by day 7 and the peak increase in bud number was observed on 21 DAP in 25 cm × thick stem treatment. The treatment of 50 cm × thick stem showed sharp increase in the development of branch number at 90 DAP proving it to be the most effective treatment. The results of comparative study showed that with the increase in number of branches, the number of buds decreased. However, branch development was comparatively slower than that of buds. The length of branch was found increased gradually with the increase in DAP, and it was significantly higher in the longer cuttings than that in shorter ones. Similarity The number of leaf was found to increase gradually with the increase in DAPs and it was significantly higher in longer cutting than that in shorter ones. The interaction effect on the increase in number of leaves also showed almost similar trend as that of number and length of branches.

Knowledge Management: Journal