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Screening of BRRI Gene Bank Germplasms for Cold Tolerance at Seedling Stage Under Natural Condition

Dr. Md. Mamunur Rashid
Senior Scientific Officer
Plant Physiology Division, BRRI, Gazipur-1701, Bangladesh

Dr. Rumena Yasmeen
Principal Scientific Officer
Plant Physiology Division, BRRI, Gazipur-1701, Bangladesh

Dr. Md. Khalequzzaman
Chief Scientific Officer
Plant Physiology Division, BRRI, Gazipur-1701, Bangladesh

Dr. Jiban Krishna Biswas
DG
Plant Physiology Division, BRRI, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

A study was carried out through culture solution to find out cold tolerant rice germplasms at seedling stage at Bangladesh Rice Research Institute. The mean score was considered for the selection of a particular genotype. Rice genotypes having a mean score of <5 were considered as tolerant to low temperature at seedling stage. Among 200 genotypes, some 41 genotypes (BRRI Acc no-208, BRRI Acc no-210, BRRI Acc no-212, BRRI Acc no-217, BRRI Acc no-222, BRRI Acc no-233, BRRI Acc no-244, BRRI Acc no-247, BRRI Acc no-253, BRRI Acc no-254, BRRI Acc no-255, BRRI Acc no-256, BRRI Acc no-271, BRRI Acc no-273, BRRI Acc no-275, BRRI Acc no-288, BRRI Acc no-296, BRRI Acc no-305, BRRI Acc no-306, BRRI Acc no-307, BRRI Acc no-311, RRI Acc no-318, BRRI Acc no-319, BRRI Acc no-330, BRRI Acc no-331, BRRI Acc no-335, BRRI Acc no-337, BRRI Acc no-339, BRRI Acc no-363, BRRI Acc no-367, BRRI Acc no-369,BRRI Acc no-389, BRRI Acc no-395, BRRI Acc no-400, BRRI Acc no-415, BRRI Acc no-433, BRRI Acc no-439, BRRI Acc no-441, BRRI Acc no-471, BRRI Acc no-472, BRRI Acc no-473) showed visual score 3 to 5 that is tolerant to moderately tolerant. Above all, BRRI acc no-472 was the most tolerant which showed mean score of 3. However, other genotypes showed visual score 7 to 9 that is susceptible to highly susceptible.

Keywords: Cold tolerance, Gene bank, Rice germplasm, Seedling stage

Location: Net house BRRI Gazipur

Start Date: 01-11-2013

End Date: 30-06-2014

Program Area: Variety and Species

Commodity: Rice

Objectives:

To identify rice germplasm which can tolerate low temperature at seedling stage.

Methodology:

Sprouted seeds of 200 rice germplasm collected from BRRI gene bank along with 3 check varieties namely BR18, BRRI dhan28 and BRRI dhan36 were sown on December 15, 2013 in netted bed attached with Styro-foam sheet floating in a tray containing full strength culture solution in the net house of Plant Physiology Division. The pH level of culture solution was maintained at 5.0 throughout the experimental period. Each entry was sown in a line and 10 seedlings in each line were maintained. Seedlings were grown up to 40 days and scored following the SES for cold tolerance of rice.

SCALE according to SES

1 = Seedlings dark green

3 = Seedlings light green

5 = Seedlings yellow

7 = Seedlings brown

9 = Seedlings dead

Source of Information: Proceedings of the BRRI Annual Internal Review for 2013-2014

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Among 200 tested genotypes 41 genotypes (BRRI Acc no-208, BRRI Acc no-210, BRRI Acc no-212, BRRI Acc no-217, BRRI Acc no-222, BRRI Acc no-233, BRRI Acc no-244, BRRI Acc no-247, BRRI Acc no-253, BRRI Acc no-254, BRRI Acc no-255, BRRI Acc no-256, BRRI Acc no-271, BRRI Acc no-273, BRRI Acc no-275, BRRI Acc no-288, BRRI Acc no-296, BRRI Acc no-305, BRRI Acc no-306, BRRI Acc no-307, BRRI Acc no-311, RRI Acc no-318, BRRI Acc no-319, BRRI Acc no-330, BRRI Acc no-331, BRRI Acc no-335, BRRI Acc no-337, BRRI Acc no-339, BRRI Acc no-363, BRRI Acc no-367, BRRI Acc no-369,BRRI Acc no-389, BRRI Acc no-395, BRRI Acc no-400, BRRI Acc no-415, BRRI Acc no-433, BRRI Acc no-439, BRRI Acc no-441, BRRI Acc no-471, BRRI Acc no-472, BRRI Acc no-473) were selected for further studies.

Knowledge Management: Report/Proceedings