Md. Iqbal Hossain
TCRC, BARI, Joydebpur, Gazipur
M.A. Kashem
Plant Pathology Division, BINA, Mymensingh
I. Hossain
Department of Plant Pathology, BAU, Mymensingh
M.M. Karim
HARS, BARI, Khagrachari
Activator, Compost, Management, Seedling diseases, Common bean
Greenhouse of Seed Pathology Centre (SPC), and in the Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh
Pest Management
The experiments were conducted both in the Greenhouse of Seed Pathology Centre (SPC) and in the Laboratory of Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh during February to August 2005. BAU-Biofungicide, Agro-wastages viz: disposed tomatoes, Cabbage leaves, and Banana peels were collected from the different departmental laboratories of BAU, Mymensingh, Bangladesh. Seed sample of Country Bean cv. BARI Seem-1 was collected from Natun Bazar, market of Mymensingh. Cabbage leaves, disposed tomato fruits and banana peels were cut into small pieces and placed in plastic container. The materials in plastic container were then placed at room temperature for two weeks. Then the decomposed materials were dried in the greenhouse using melamine plates for 4 days. The dried materials were grinded in powder. BAU-Biofungicide was used with agro-wastages in 1:3 (BAU-Biofungicide:agro-wastages) ratios for preparing the compost. The following sixteen treatments were used: T1 = Control, T2 = Compost prepared by disposed tomato fruits, T3 = Compost prepared by cabbage leaves, T4 = Compost prepared by peel of banana, T5 = T2 + T3, T6 = T3 + T4, T7 = T2 + T4 , T8 = T2 + T3 + T4 , T9 = BAU-Biofungicide, T10 = T2 + T9, T11 = T3 + T9, T12 = T4 + T9, T13 = T2 + T3 + T9, T14 = T3 + T4 + T9, T15 = T2 + T4 + T9, T16 = T2 + T3 + T4 + T9. Soil was collected from the Field Laboratory of the Department of Plant Pathology, Bangladesh Agricultural University (BAU), Mymensingh and the collected soil was dried. The dried soil was sterilized with formalin (40%) at the rate of 5 ml formalin diluted with 12 ml of water for four kg soil. The formalin treated soil was covered with polythene sheet for 48 hrs and then exposed of 72 hrs for aeration before setting the experiment. For each treatment, soils of 4 trays were drenched with subsequent prepared compost except BAU-Biofungicide and control treatments. The prepared composts were given in soil at 6 g/kg soil. Two hundred seeds were taken in a beaker and 25 drops of water was added to it for uniform moistening the seed surface. Seeds were then treated with BAU-Biofungicide as per treatment @ 25 g/kg seed. The treated seeds as well as non-treated seeds were sown in row in trays (50 seeds/tray) as per treatment used. There were four replications for each treatment. The experiment was conducted following Completely Randomized Design. The following data were recorded : (a) germination (%), (b) germination failure(%), (c) normal seedlings(%), (d) abnormal seedling(%), (e) disease free healthy seedlings (%), (f) anthracnose diseased seedling (%), (g) post-emergence death (%), (h) Shoot weight (g), (i) Root weight (g), (j) Shoot length (cm), (k) Root length (cm) and (l) Vigour index (%). Vigour index was calculated at 10, 15 and 20 DAS by using the formula of (Baki and Anderson, 1973) as shown below: Vigour index (VI) = (Mean shoot length + Mean root length) x % germination.
J. Agrofor. Environ. 4 (2): 159-162, 2010; ISSN 1995-6983
Journal