Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Growth and Reproductive Responses of Brown Plant Hopper to Five Rice Cultivars

M.A. Hossain
Shetu Corporation Limited, Uttara, Dhaka-1230, Bangladesh

K.S. Ahmed
Department of Entomology, BAU, Mymensingh,

M.L.R. Mollah
Entomology Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh

R. Islam
Oilseed Research Center, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur

Abstract/ Executive Summary:

Growth and reproductive responses in terms of nymphal period, survival rate, growth index, oviposition, hatchability of eggs, fecundity and population growth of brown planthopper, Nilaparvata lugens to five rice cultivars were studied during March to September, 2006 in Entomology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh. Brown planthopper nymphs caged on T27A, Swarnalata and ARC10550 had reduced survival while 100 percent nymphs survived on susceptible TN1. Growth index was lowest on T27A while it was highest on TN1. Maximum number of 48.8 eggs were laid on susceptible TN1 while the lowest was on resistant variety ARC10550 and T27A. TN1 favoured egg hatchability but T27A adversely affected the same. The highest fecundity of 493 eggs was recorded on susceptible TN1 and the lowest of the same was only 83 on resistant T27A followed by Swarnalata and ARC10550. Brown planthopper population development was highest on TN1 having 330 insects but Swarnalata and T27A had adverse effects on population increase having only 51 and 73 insects, respectively.

Keywords: Nilaparvata lugens, Nymphal development, Growth index, Fecundity, Population growth

Location: Entomology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh

Start Date: 00-03-2006

End Date: 00-09-2006

Program Area: Pest Management

Commodity: Rice

Objectives:

1. To investigate the responses of brown planthopper to five rice cultivars.

Methodology:

The differential response of brown planthopper was studied on five selected rice cultivars. A series of experiments were conducted at the growth room under controlled environmental condition (27±2^oC temperature, 60-70% relative humidity with 12h photoperiod) in Entomology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. The rice cultivars were IR64 with bph1, ARC10550 with bph5, Swarnalata with bph6, T27A with unknown dominant single gene and TN1 having no resistant gene to brown planthopper. The response tests included nymphal growth and reproduction of female brown planthopper on different cultivars of rice. To obtain a regular supply of the insect of different stages, brown planthopper was mass-reared in the controlled growth room on susceptible rice variety TN1 following standard culture technique. The growth aspects of brown planthopper nymphs included its developmental period, survival rate and growth index on each of the five cultivars of rice. To determine the nymphal developmental period, 20-day old potted plants of the test cultivars caged with mylar film were infested with ten freshly hatched first instar nymphs and arranged in a completely randomized design. Five pots for each cultivar were used in this study, each pot represented one replicate. The duration of the test was extended to allow nymphs to reach the adult stage. Mean developmental period (1st instar nymph to adult) was calculated based on the daily adult emergence until all the surviving nymphs emerged to adults in each cage. Another experiment was conducted to determine the survival of brown planthopper nymphs following same methodology and design as described for nymphal period. The cages were monitored daily and sixteen days after infestation, when the nymphs became adults on susceptible plants, data were recorded on the survival rates. Growth study of N. lugens was initiated by infesting 30-day old potted rice plant of each cultivar caged by mylar film with ten first instar nymphs. The hoppers growth index on tested cultivars was calculated by the following formula. Growth index = % adult emergence/Mean developmental period in days. The reproductive aspects of brown planthopper females included its oviposition, hatchability of eggs, fecundity and population growth on each of the five cultivars of rice. Ovipositional response of brown planthopper was studied by releasing one pair of gravid females on thirty day old potted plant caged by mylar film represented one replicate. The females were allowed to oviposit for 24 hours after which the plants were removed. After five days, the number of eggs laid by the females was counted by dissecting the leaf sheath under a binocular microscope. The experiment was set up in a completely randomized design with five replications. Hatchability of eggs of N. lugens was investigated following same design as that described for the ovipositional response test. Single pair of 5-day old gravid females reared on susceptible TN1 plants was caged on tested cultivars. The females were allowed to oviposit for 24 hour after which the hoppers were removed. The plants were left caged with mylar film for 10 days, which was longer than the insects normal incubation period. The total number of nymphs that emerged on the test plants was recorded. At the end of nymph emergence, unhatched eggs were counted by dissecting the leaf sheath under a bionocular microscope. The total number of eggs laid per plant was determined by summing the total number of nymphs and unhatched eggs. Then percent egg hatchability was calculated by the following formula: % egg hatchability = [no. of nymphs emerged/(no. of nymphs emerged + no. of unhatched eggs)] x 100. To observe the fecundity of brown planthopper, rice plant caged with mylar film in a pot was infested with single pair of newly emerged males and females representing one replicate. The total number of nymphs emerged on plants was recorded and represent the number of viable eggs produced by the females during their lives. The experiment was set up in a completely randomized design with three replications. Population growth of brown planthopper was studied in a separate experiment where 30-day old potted plants of each cultivar caged with mylar film (90cm x 10cm) were infested with three pairs of three-day old of brown planthopper females. Three pots for each cultivar were infested; each pot served as a replicate. The cages were checked at early hours of the day for dead hoppers, which were replaced. After 21 days of initial infestation, the adults were removed from the cages, and the progenies were collected with aspirator into a plastic container from each cage. The containers were frozen for 24 hours and then the nymphs were counted for populations build up of N. lugens. Data obtained from the experiments were analysed using computer package programme, MSTAT-C and means were ranked by Duncan’s Multiple Range Test (DMRT).

Source of Information: J. Agrofor. Environ. 4 (2): 185-188, 2010; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, survival and growth index of brown planthopper nymphs are found to be reduced on resistant rice cultivars compared to susceptible TN1. The resistant cultivars had also very adverse effects on fecundity as well as on population growth while it was favoured by TN1.

Knowledge Management: Journal