Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Comparative Efficacy of Alcoholic Extracts of Black Peppers (piper Nigrum) and Chutra Leaves (urtica Dioica) with Esb3 Against Coccidiosis in Chickens

Bayzer Rahman
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Tahmina Begum
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Yousuf Ali Sarker
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Quamrul Hassan
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Sukumar Saha
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Mahmudul Hasan Sikder
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Abdul Awal
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The present study is undertaken to compare the efficacy of alcoholic extracts of Black Peppers (Piper nigrum ) and Chutra leaves (Urtica dioica ) with a patent drug Esb3 against coccidiosis in chicken. 16 Fayoumi breed chickens were collected from a local farm and divided into four groups; A, B, C and D, each consisting of four chickens, Group A (control), Group B (alcoholic extracts of Black Peppers @ 9ml/kg bd wt.), Group C (alcoholic extracts of Chutra leaves @ 9ml/kg bd wt.) and Group D ( Esb3 @ 1 ml/ liter drinking water). All the treated chickens were kept under close observation for 18 days and data was collected at 3 days interval. In group B, two chickens died within 4-7 days of treatment and in group C one chicken died on 5th day of medication. All the chickens of control group died within 5-7 days of medication. Oocyst was counted for per gram of feces in all groups. Biochemical parameters like SGPT and haematological parameters like Total erythrocyte count (TEC), Haemoglobin count (Hb), Packed cell volume (PCV), Erythrocyte sedimentation rate (ESR) were determined. There were significant decrease in oocyst count in group B and C in compared to control and very few oocysts were present in faeces of 6 days onward of medication. All the chickens were survived in group D and oocyst started to disappear in the faeces from 3rd day onward of medication. Our study suggests Both Black Peppers and Chutra are effective against coccidiosis in chicken and Chutra is more effective than Black peppers.

Keywords: Efficacy, Medicinal plants, Coccidiosis, Chicken

Location: Department of Pharmacology and Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Start Date: 00-00-2014

End Date: 00-00-2014

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

1. To evaluate the efficacy of plant extracts (Black Peppers and Churta leaves) compared to ESB3 drug in poultry coccidiosis.

Methodology:

The experiment was conducted in the Department of Pharmacology and Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. Experimental Chickens - Total 16 chickens of Fayoumi breed were collected from a local farm. The chicks were 3-4 weeks of age and weighing 250-300 gm. The experimental poultry shed was properly brushed with broom and then washed by forced water using a hosepipe. The room was disinfected with bleaching powder and it was left for 7 days. After the interval, the shed was again disinfected with Virkons (Antes International Limited, England). Additionally the poultry shed was also fumigated by formalin and potassium permanganate for a period of 24 hours for disinfection. Collection of Coccidia Affected Birds and Isolation of Oocyst and Infection - Four chickens suffering from coccidiosis were collected from a private farm. The ceca of coccidian infected chickens were separated and opened with scissors and forceps and the fecal contents were taken out in a petri dish containing water. The content was then stirred for 20 minutes and filtered. 50 ml of filtrate was taken in a glass jar. A drop of filtrate was examined under the microscope to observe oocyst. In 50 ml of filtrate 1 gm of potassium dichromate was added and mixed thoroughly and was kept for 24 hours for sporulation. After 24 hours, a drop of filtrate was examined under the microscope to observe sporulatedoocyst. The oocysts were counted by McMaster Egg counting method. All experimental birds were divided into four groups and were kept in four different cages. Oocyst containing filtrate was fed to all birds of the groups at the dose rate of 20 ml / bird. All the birds become infected within 3-5 days. The infection of the bird was confirmed by observing the visible symptoms and also by faecal egg count per gm by McMaster Egg counting method. Alcoholic Extracts of Black Peppers and Chutra - The leaves of Chutra and whole of black Peppers were collected in fresh condition. They were washed thoroughly with fresh water. These were cut into small pieces, sundried and grinded in a grinding machine. The powdered sample was measured with a balance. 100 gm of each sample was taken and preserved in air tight bottle separately. Alcoholic extracts of Black Peppers and Chutra were obtained by Soxhlet method (250 ml of alcohol was used for 100 gm of powdered sample). Determination of Biochemical and Hematological Parameters - Biochemical parameters like serum glucose and SGPT (serum glutamic pyruvic transaminase) were determined by using autoanalyser (Model No. Reflotron M-06). Haematological parameters like Total Erythrocyte Count (TEC), Haemoglobin count (Hb), Packed Cell Volume (PCV), Erythrocyte Sedimentation Rate (ESR) was determined. The parameters were determined. Statistical Analysis - The data was analysed statistically between normal and treated values by student’s t test and a p value of ≤ 0.5 was considered significant.

Source of Information: Res. Agric. Livest. Fish. Vol. 2, No. 1, April 2015: 117-124; ISSN : P-2409-0603, E-2409-9325

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It can be concluded that alcoholic extracts of Chutra leaves at the dose rate of 9 ml/kg was found to be most effective (75%) nearer to patent drug Esb3 in treating the chicks experimentally infected with coccidiosis. Alcoholic extracts of whole of Black Peppers proved less effective (50%) than those of alcoholic extracts of chutra leaves and patent drug Esb3. Further study should commence to investigate the mechanism of action of Black Peppers and Chutra leaves as anticoccidial in poultry.

Knowledge Management: Journal