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Immune Response of Fowl Cholera Vaccine Produced at Bangladesh Agricultural University in Farm Level

Md. Abdus Sattar Bag
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Md. Mansurul Amin
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Md.Bahanur Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Yasir Ahammad Arafat
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Md. Salim
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Imrul Hasan Rasel
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Ummay Habiba Majumder
Department of Microbiology and Hygiene, Faculty of Veterinary Science, BangladeshAgricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The work was carried out to determine the immunogenicity of fowl cholera vaccine (FCV) produced by Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) at Bangladesh Agricultural University (BAU). A total five hundred of sevenweek old Hy-sex chickens (both white and brown) were vaccinated @ 0.5 ml of 2.93×108 CFU through subcutaneous route in each selected groups such as A1, A2 and A3; and B1, B2 and B3. Booster dose was provided at 13 weeks of age in group A3 and B3. Group C was kept as unvaccinated/control. Postvaccination sera were collected at different time schedule from all the groups of birds and antibody against fowl cholera were determined by Passive haemagglutination (PHA) test. At 4 weeks of primary vaccination (11 weeks aged birds) the mean PHA titres of sera were 96.00±34.21 and 96.00±34.21in group A1 and B1 respectively. On the other hand mean PHA titres at 5-weeks following vaccination (12 weeks aged birds) were 88.00±33.12 and 96.00±34.21in group A2 and B2, respectively. After 4 weeks of booster vaccination the mean PHA titres were 104.00±33.12in A3 and 104.00±33.12 in B3 group. The mean PHA titres in chickens of unvaccinated control group C was <4±0.00.Fowl cholera vaccine prepared at LPVRPC induced a good level of immunity at the farm level and it was also demonstrated that booster (secondary) vaccination is essential to develop protective level of immunity.

Keywords: Fowl cholera ,Vaccine Immunogenicity, PHA titres, Antibody

Location: Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Start Date: 00-07-2010

End Date: 00-12-2010

Program Area: Animal Health and Management

Commodity: Diseases

Objectives:

1. To isolate and identify P.multocida from naturally infected chicken and to determine on farm immunogenicity of adjuvant fowl cholera vaccine produced by LPVRPC.

Methodology:

Experimental design - The present research was conducted during the period of July 2010 to December 2010.Fowl cholera vaccine as prepared by LPVRPC at BAU was investigated immunogenicity for measured in term of production of antibody in vaccinated chicken determined by PHA test. Seven weeks aged Hy-sex chicken (white and brown) were selected for these experiment. These chicken were divided into two groups- (Vaccinated group and Unvaccinated group) under this vaccinated group collection of blood prior to vaccination from Hy-sex brown A and Hy-sex white B. Primary vaccination were done at 6 weeks of age in Hy- sex brown A and Hysex white B. Post primary vaccination bleeding occurred at 10 weeks of age (A1 and B1) or 30 days after primary vaccination and Prebooster bleeding at 11weeks of age (A2 and B2) or 37 days after primary vaccination. Booster vaccination of Hy-sex brown A and Hy-sex white B were done at 12 weeks of age. Post booster bleeding at 16 weeks of age (A3 and B3) or 74 days after primary vaccination. Then collection of sera and PHA test was performed. Passive haemagglutination (PHA) test - The test was used to determine the antibody titers in chickens and was performed according to the standard methods. The sensitivity of PHA test depends upon the use of soluble antigens. In this case, capsular antigens (soluble antigen) of P. Multocida were coupled to chemically modified erythrocytes (sheep erythrocytes) and then antigen-coated erythrocytes readily react with specific antibodies and results in haemagglutination. Microtitre plate method - The procedure of the PHA test was followed according to the method described by Tripathy et al., .An amount of 50 μl of PBS was first poured in each well up to 8th well of horizontal row of microtitre plate. 50 μl of test serum was added in the 1st well. Two fold dilutions of serum ranging from 1: 2 to 1: 256 were made by transferring 50 μl of the mixture from the 1stwell to 2nd well and thus continuing successively up to the 8th well from where an excess amount of 50 μl of the mixture was poured off. A volume of 50 μl 0.5% somatic antigen sensitized hRBC was taken in each of the eight wells. The Control system, horizontal row of microtitre plate (9th well: equal volume of 50 μl of normal serum and PBS and10th well: equal volume of 50 μl of sensitized tanned RBC and PBS). The content of the wells of the test system and control were mixed by gentle agitation of the microtitre plate and kept at room temperature for 4 to 5 hours. The PHA titre was the highest dilution of test sera were complete haemagglutination occur due to the reaction of specific antibody and antigen sensitized tanned HRBC. The results were recorded by deposition of a diffuse thin layer of clumping of RBC on the bottom of the wells, which indicated HA positive, and a compact buttoning with clear zone indicated HA negative. The reciprocal of the highest dilution of sensitized tanned HRBC was considered as titre of the serum.

Source of Information: Res. Agric. Livest. Fish. Vol. 2, No. 1, April 2015: 103-107; ISSN : P-2409-0603, E-2409-9325

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Fowl cholera vaccine produced by Livestock and Poultry Vaccine Research and Production Centre (LPVRPC) at BAU induced a good level of antibody in layer chicken at farm level. The vaccine produced higher level of antibody when booster dose was given after primary vaccination.

Knowledge Management: Journal