Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Dose Optimization with Pituitary Gland Hormone for Induced Breeding of Bata Fish (labeo Bata)

M. I. Miah
Dept. of Fisheries Management, Bangladesh Agricultural University Mymensingh-2202, Bangladesh

A. A. Mamun
Dept. of Fisheries Management, Bangladesh Agricultural University Mymensingh-2202, Bangladesh

M. M. R. Khan
Dept. of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202,Bangladesh

M. M. Rahman
Dept. of Fisheries Management, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

An experiment on induced breeding of bata fish (Labeo bata) was carried out to determine the optimum dose of pituitary gland hormone. The present study consisted of four treatments namely T₁, T₂, T₃, and T₄ with three replications of each. Twenty-four pairs of male and twelve pairs of female were selected from the brood rearing ponds and the average body wt of the female and male were 390±10 and 258±9 g respectively. To observe the effective dose for induced breeding, the females were first injected at the rate of 0.5 (T₁), 1.0 (T₂), 1.5 (T₃) and 2.0 (T₄) mg PG/kg body wt. while the second doses were performed at the rate of 4.0 (T₁), 4.5 (T₂), 5.0 (T₃), and 5.5 (T₄) mg PG/kg body wt. after 6 h from the first dose. On the other hand the males were administered a single dose of 2 mg PG/kg body wt. at the time of second dose of the female. The ovulation rate, fertilization rate, hatching rate and survival rate were determined. Among the four treatments T₂ (1.0 mg PG/ kg body wt.) showed the best result in terms of ovulation rate (89%), fertilization rate (84%), hatching rate (85%) and survival rate (84%). The dose of 1.0 mg PG/kg body wt. can be used in induced breeding of bata fish (L. bata) for the development of hatchery propagation.

Keywords: Dose, Pituitary gland, Induced breeding, Labeo bata

Location: Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh.

Start Date: 15-06-2006

End Date: 01-08-2006

Program Area: Animal Health and Management

Commodity: Fish, Fish

Objectives:

1. To determine the optimum dose of pituitary gland hormone.

Methodology:

The major part of the experiment was conducted at the Hatchery of the Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. The experiment was done from 15 June to 1 August 2006. Collection of fish - About 200 L. bata both males and females were collected from the Jamuna river near Sirajgonj district. Collected fish were stocked in the previously prepared pond during November-December 2005. They were selected for brood stocking considering size, shape and colour. Brood rearing - The brood fish of L. bata were reared in rectangular ponds of size 18x14 m² and an average depth of 1.3 m, situated in the area of Field Laboratory complex of Fisheries Faculty. Special dieting was initiated in early March and continued up to the completion of breeding activity up to August 2006. Special feed enriched with protein and vitamin E was formulated which enhances the gonadal maturation in fishes. The feed was supplied at 5% body wt. of the brood fish two times a day at 0900 h and 1700 h respectively. The feed used during the period of brood management was analyzed to know their proximate composition. Regular manuring with cowdung was done at 15 days interval @ 1235 kg/ha. and fertilization was done with Urea and TSP @ 49 kg/ha. and @ 24 kg/ha. respectively. Liming was performed whenever necessary @ 247 kg/ha. Experimental design - For induced breeding of L. bata, broods were collected from the brood rearing ponds of the Field Laboratory complex. In this experiment, four doses of PG hormone 0.5,1.0,1.5 and 2.0 mg/kg body wt. considered as treatments T₁, T₂, T₃ and T₄ respectively were used with three replications of each. For each treatment 12 males and 12 females were used. So 96 brood fishes were injected for induced breeding that composed of 32 females and 64 males. After ovulation, one female and one male were selected for stripping from each treatment. For dose optimization, ovulation rate, fertilization rate, hatching rate and survival rate was determined. Broad selection - Healthy mature males and females were selected carefully. The average weight of the females and males were 390±10 g and 258±9 g respectively. The mature breeders were selected. Collection and preparation of PG - Locally available dry carp pituitary glands (PG) were collected from market in preserved condition in airtight vials were used as inducing agent. At first, the pituitary glands were gently removed from the vial with a pair of forceps and dried by using the filter paper for 2-3 minutes and then weighted by an analytical electronic balance (College B204-S, Switzerland). The amount to be weight out was calculated on the total of the body weight of all the fishes using the following formula : Weight of PG (mg) = (Wt x Pt)/1000. Where, Wt represents total body weight (g) of all the fishes to be injected and Pt, represent the rate in mg PG to be injected/kg body weight under a particular treatment. The weighed PG were transferred to a tissue homogenizer for thoroughly crushed. The crushed PG then diluted in distilled water to dissolve it and was centrifuged with a hand centrifuger for precipitation. The freshly prepared supernatant solution of hormone was then taken slowly in a 1 ml hypodermic syringe for injection. Hormone administration - After preparation of PG solution, it was injected in brood fish. Fish was caught carefully by net, and kept in sponge. They were covered by soft cloth; PG was then injected near the pectoral fin base . The amount of PG solution for each fish was determined before according to the body weight of the broods. First dose of PG was administered to the female at 0600 h, at six hours interval; second dose of PG was given to female at 1200 h and first dose to male. Doses of PG for male and female broods - In this experiment, four different doses of PG were applied. of the broods. First dose of PG was administered to the female at 0600 h, at six hours interval; second dose of PG was given to female at 1200 h and first dose to male. Ovulation rate, fertilization rate and hatching rate were determined. Determination of survival rate - Five days old spawns of L. bata were stocked in glass aquarium (45 x 25 x 24 CM) containing 17 liters of deep tube-well water in each. The experiment was designed with four treatments (TI, T2 T3 and T4) and each treatment contained three replications (R₁, R₂ and R₃) based on the larvae of four hormone treatments of L. bata. Sixty larvae of T₁, T₂ T₃ and T₄ were used at a stocking density of 3.5 spawns/ L of the volume of water and were reared for 30 days. All other conditions during the experimentation were maintained in same condition. After completion of the experiment at 30th day, the number of total live larvae in aquarium was counted separately for calculation of survival rate. Statistical analysis - The results found in the experiment were subjected to statistical analysis, ANOVA, (one way) that showed the significance (P<0.05) level of differences between the treatments. This statistical analysis was performed with the aid of the computer software MS Excel program. Significant results (P<0.05)were further tested using Duncan’s New Multiple Range Test (DMRT) to identify significance differences among means. This statistical analysis was performed with the computer software MSTATC program.

Source of Information: Bang. J. Anim. Sci. 2008, 37 (1) : 70 - 77; ISSN 0003-3588

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It can be said in short, that the ovulation, fertilization and hatching rate and also the survival rate of larvae differs mainly due to the hormone dose as well as brood stock management, quality of brood, quality of eggs, seasonal variation, incubation density, water flow during incubation, quality of hatchery water and handling procedure of the broods. But upon all consideration PG of 1.0 mg as first dose and 4.5 mg as second dose for per kg body weight may be recommended for induced breeding of L. bata in hatchery.

Knowledge Management: Journal