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Survival of Antibiotic Resistant Pseudomonas Strains in Different Types of Water

M. S. Islam
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

M. B. R. Chowdhury
Department of Aquaculture, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh

Abstract/ Executive Summary:

A study was conducted to investigate the survival of five Pseudomonas strains resistant to antibiotics in different types of water. The selected Pseudomonas strains were designated as strain P1(CT-29), strain P2(CT-25), strain P3(CT-36), strain P4(CT-20) and strain P5(CT-27) which were only recovered from farmed fishes. Six types of water viz., distilled water, saline water, tap water, deionised water, pond water and river water were used. Among these experimental waters, river water was found to be the most suitable for long-term survival of these strains. Deionised water did not support survival of all these Pseudomonas strains. Pond water, tap water and distilled water were moderately suitable for strain P1 and strain P4. Saline water was also found to be highly suitable for long-term survival in case of the strain P3 and moderately suitable for normal survival of strain P2 and strain P5.

Keywords: Pseudomonas, Water, Antibiotic

Location: Department of Aquaculture, Bangladesh Agricultural University

Start Date: 00-00-1993

End Date: 00-00-1993

Program Area: Animal Health and Management

Non-commodity: Performance

Objectives:

To investigate the survival of five selected antibiotic resistant Pseudomonas strains in different types of water

Methodology:

Bacterial strain - Five bacterial strains were used for survival test. These were as follows: 1. Strain P1 (CT-29) 2. Strain P2(CT-25) 3. Strain P3(CT-36) 4. Strain P4(CT-20) 5. Strain P5(CT-27). Experimental water - Six different types of water were used as experimental water for survival test of the five selected Pseudomonas strains. These were as follows: 1. Distilled water 2. Saline water 3. Tap water 4. Deionised water 5. Pond water (lsha Khan Lake, BAU campus) 6. River water (Brahmaputra river, near BAU campus) The waters were used for the survival test after steri I ization by autoclave. Parameters like dissolved oxygen and pH of the relevant water were recorded before and after autoclaving. Procedures of survival test - Individual experimental. Pseudomonad isolates were cultured on TSA plate. Then a sample of freshly cultured (18-24 h) inoculum weighing 20-30 mg (cfu) was taken into the sterile test tube containing 3-4 ml of distilled water to make a stock suspension. Then 0.5 ml of suspension was inoculated into 150 ml of sterile individual experimental water from the stock suspension and maintained at 25°C in incubator. At each time of sampling 0.2 ml of incubated bacterial suspension was taken separately for individual and required ten-fold dilutions were made in sterile relevant experimental water. From required each dilution 0.1 ml of fluid was taken for inoculation on TSA plate and spreaded it by L-shaped sterile glass rod. Then the plates were placed at 25°C in the incubator for 24-36 hours to incubate. After 24-36 hours incubation, the number of colonies were counted by colony counter. Viable number of bacteria were determined at 0 day (immediately after incubation), 1 day, 3 day, 7 day and 10 day until completion of the experiment. In each circulation duplicate plates were used and average total load of bacteria were counted.

Source of Information: Bangladesh J. Fish. Res., 1 (2) : 39-45, 1997

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

1. The present study provides useful information for the fish culturists on the survival of the Pseudomonas bacteria (fish pathogens) and with this knowledge, a fish culturist could possibly change the water in order to reduce the incidence of the bacteria. 2. Further studies are necessary to know the pathogenicity of these Pseudomonas strains to various species of fish and to find out an appropriate control measures against the recovered pathogen.

Knowledge Management: Journal