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In Vitro Clonal Propagation of Hybrid Acacia (a. Auriculiformis X A. Mangium)

Ratan Lal Banik
Bangladesh Forest Research Institute, P. O. Box 273, Chittagong-4000, Bangladesh.

S. A. M. Nurul Islam
Bangladesh Forest Research Institute, P. O. Box 273, Chittagong-4000, Bangladesh.

Abstract/ Executive Summary:

A protocol for regeneration of plantlets from hybrid acacia (Acacia auriculijormisxA. mangium) trees was achieved through tissue culture technique. Effects of MS and B5 culture media supplemented with various concentration of different phytohormones were tested for the induction and proliferation of shoots. The etiolated shoot was cultured and proliferation was found best on MS medium supplemented with BAP (1mg/I). Proliferation and growth of shoots were further improved through subculture. These shoots produced well developed roots when cultured on 1/2 MS containing 1.5 mg/I IBA supplemented with activated charcoal. Ninety per cent regenerated plants survived in the field.

Keywords: Hybrid acacia, Etiolated shoot explant, In vitro culture, Propagation

Location: Chittagong

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Akash moni

Objectives:

To develop in vitro propagation technique for mass scale production of planting stocks of A. auriculiformisxA. mangium hybrid.

Methodology:

A number of hybrid acacia trees were identified and selected from a 5 years old fuel wood plantation of A. mangium and A. auriculiformis at Harbang forest area of Chittagong Forest Division. One of the selected trees was felled and stump was covered by sand in the mid of June 1994 and it coppiced within two weeks. After a month, the young and etiolated sprouting shoots were collected. The etiolated shoots were sterilized with HgCI₂ (0.1%) and ethanol (70%). Sterilized shoots were sized (0.5-1.5 cm) to make explants and washed with sterile distilled water. The two basal media, Murashige and Skoog (1962) (MS) and Gamborg and Eveleigh (B5), were used to culture the explants in in vitro condition. Both the media were agar gelled using 5 g agar per litre in 150 mmx20 mm test tubes. Approximately 10 ml medium of each kind was used in the tube. The filter paper bridge was al used in 150x20 mm test tubes containing 10 mI liquid MS medium. The growth regulators namely BAP (0.1, 1.0,5.0 mg/l), Kn (1.0 mg/I), 2iP (1.0, 10.0 mg/I), NAA (0.1 mg/l) and IBA (0.1, 1.0,1; mg/I) were supplemented with basal media. The pH of the media was adjusted at 5.8 before autoclaving. The temperature of the growth room was regulated to 27±3°C and for illumination three fluorescent tube lights (40 W) and two incandescent bulbs (25 W) were used. The 16 hrs light and 8 hrs dark periods were regulated.

Source of Information: Bangladesh Journal of Forest Science, Jan-Dec. 1996, Vol.25(1&2), pp:1-7, ISSN:1021-3279.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The etiolated shoot was cultured and proliferation was found best on MS medium supplemented with BAP (1mg/I). Proliferation and growth of shoots were further improved through subculture. These shoots produced well developed roots when cultured on 1/2 MS containing 1.5 mg/I IBA supplemented with activated charcoal. Ninety per cent regenerated plants survived in the field.

Knowledge Management: Journal