Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Reduction of Bacterial Pathogens in Penaeus Monodon and Macrobrachium Rosenbergii Using Several Chemical Interventions

Abu Hena Muhammad Yousuf
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Md. Kawser Ahmed
Associate, Professor
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Sabina Yeasmin
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.

Nazmul Ahsan
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.

Md. Mujibur Rahman
Department of Microbiology, Stamford University, Dhaka, Bangladesh.

Md. Monirul Islam
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

A study was undertaken to determine the most effective element among the commonly used antimicrobial agents, especially in the body muscle of black tiger shrimp ( Penaeus monodon ) and giant freshwater prawn ( Macrobrachium rosenbergii ) on the basis of different chemical interventions. Using 10 ppm calcium hypochlorite, the microbial load has been reduced more significantly (p>0.001) than trisodium phosphate, lactic acid, oxalic acid, sodium carbonate, potassium bicarbonate and acetic acid and as result the total plate count in the Penaeus monodon and Macrobrachium rosenbergii samples reduced by on average of 3.83 (99.53%) and 3.24 (95.5%) log₁₀ CFU/g, respectively. Moreover, the aforementioned compound was also found to be very effective against Escherichia coli and Vibrio spp i.e., the count of E. coli and Vibrio spp. reduced to nil or nearly nil .

Keywords: Reduction of bacteria, Chemical interventions, Black tiger shrimp, Giant freshwater prawn

Location: Cox’s Bazar and Satkhira

Start Date: 00-12-2005

End Date: 00-04-2006

Program Area: Pest Management

Commodity: Shrimp, Diseases

Objectives:

1. To evaluate the intervention of chemicals e.g. calcium hypochlorite, trisodium phosphate, lactic acid, oxalic acid, sodium carbonate, potassium bicarbonate and acetic acid.

2. To determine the most effective chemical treatment in reducing bacterial pathogens.

Methodology:

To find out the efficacy of different chemical interventions to reduce the microbial load especially on the locally available shrimp and prawn species in the present study samples were obtained from five representative sources of Bangladesh such as tiger shrimp ( Penaeus monodon ) from Cox’s Bazar and Satkhira; giant water prawn ( Macrobrachium rosenbergii ) from Noakahli; from local markets and departmental chain shops in the Dhaka city, which has been collected from December, 2005 to April, 2006. Among all the samples, some were collected instantly from the gher (earthen pond) and the others from the shopkeepers without having proper storage conditions. To avoid further contamination or spoilage, during transportation from the source to laboratory, samples were carried by special sterile bags packed in insulated box with ice to maintain the temperature around 4 to 6°C. The samples were stored at -80°C at the laboratory until use.

At first samples were deheaded and peeled and then it was rinsed thoroughly with sterile distilled water. Alongside the microbial evaluation of the sterile distilled water washing, the washed samples were also assessed for bacterial growth by mutilating, macerating and smashing into the distilled water. Dilution and plating by spread plate method were carried out soon after sampling. Approximate 15 ml of plate count agar (PCA), MacConkey agar, membrane fecal coliforms (mFC) agar, eosin methylene blue (EMB) agar, thiosulphate citrate bile salt sucrose agar, xylose lysine deoxycholate (XLD) agar has been melted and brought to 45°C was poured into the sterile Petri plates and different initiatives were taken for equal distribution of the media. After solidifying and inoculation, plates were inverted and placed in incubator at 37°C for 48 hours for evidence of growth. Total sixteen samples have been analyzed in terms of aerobic plate count (APC), enterobacteriaceae counts and Vibrio spp. counts. Gradually, these counts and Vibrio spp. counts. Gradually, these enumerated samples were chemically intervened using as 10 ppm calcium hypochlorite, oxalic acid, sodium carbonate, acetic acid, lactic acid, trisodium phosphate and potassium bicarbonate. The samples have been washed 1 min with vigorously shaking by the aforementioned chemicals. In this way each treatment has been repeated four times.

Source of Information: World Journal of Agricultural Sciences 4 (S): 856-861, 2008, ISSN 1817-3047

Article Link (Online Journal): http://www.idosi.org/wjas/wjas4(s)/10.pdf

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The comparison of antimicrobial effects of seven treatments revealed treatment of raw shrimp and prawn with calcium hypochlorite is the most effective than the other agents in reduction of total bacterial load as well as elimination of harmful bacteria. Sodium carbonate, potassium bicarbonate, lactic acid and oxalic acid were found to be less effective than acetic acid and trisodium phosphate. However, these chemicals with high concentration as a quality control measures can adversely affect the quality of shellfish due to the presence of its residues in food; moreover they will not be able to eliminate the pathogenic bacteria completely, but will reduce and increase the shelf life and quality to some extent.

Knowledge Management: Journal