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Prevalence of Microbial Load in Shrimp, Penaeus Monodon and Prawn, Macrobrachium Rosenbergii from Bangladesh

Abu Hena Muhammad Yousuf
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Md. Kawser Ahmed
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Sabina Yeasmin
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.

Nazmul Ahsan
Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh.

Md. Mujibur Rahman
Department of Microbiology, Stamford University, Dhaka, Bangladesh.

Md. Monirul Islam
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

A comparative studies regarding prevalence of microbial flora in the muscle of locally available tiger shrimp ( Penaeus monodon ) and giant water prawn ( Macrobrachium rosenbergii ) have been analyzed in terms of aerobic plate count (APC), enterobacteriaceae and Salmonella-Shigella (SS) counts. The total counts ranged from 2.04x10² to 4.5x10⁵ CFU/ml for shrimp and 1.08x10² to 1.2x10⁵ CFU/ml for prawn. The total coliforms count ranged between 5.4x10² and 8.5x10⁵ cells for the shrimp and for the prawn 5x10² and 4.4x10⁴ cells. Furthermore, the Salmonella-Shigella (SS) count ranged from 0.2x10² to 1.1x10² cells for the shrimp and between 0.26x10² and 0.96x10⁴ cells for the prawn. Sixteen isolates were characterized from all the samples on plate count agar with percentage of different microbes characterized as follows: Staphylococcus auerus (6.25%), Salmonella sp. (25%), Shigella sp. (12.5%), Flavobacterium sp. (12.5%) and Vibrio sp. (43.75%). Results revealed that the microbial load found to be higher in samples taken from the departmental chain shops, without having proper treatment.

Keywords: Food safety, HACCP, Fish borne pathogens,Microbiological quality

Location: District of Cox’s Bazar, Satkhira, Noakahli.

Start Date: 00-12-2005

End Date: 00-02-2006

Program Area: Postharvest and Agro-processing

Commodity: Shrimp

Objectives:

To evaluate the incidence of bacterial load and pathogens in locally available shrimp.

To provide potential approaches for improving the quality assurance and create awareness among the consumers.

Methodology:

Samples were obtained from five representative sources of Bangladesh such as tiger shrimp ( Penaeus monodon ) from District of Cox’s Bazar (A) and Satkhira (B) and giant water prawn ( Macrobrachium rosenbergii ) from Noakahli Dist. (E) To assess the microbial load in the present study; both were obtained from local markets (C) and departmental chain shops (D) in Dhaka city. Samples have been collected from December, 2005 to February, 2006. To avoid further contamination, during transportation from the source to laboratory, samples were carried by special sterile bags packed in insulated box with ice to maintain the temperature around 4 to 6°C. Samples were rinsed thoroughly with sterile distilled water. Alongside the microbial evaluation of the sterile distilled water washing, the washed samples were also assessed for bacterial growth by mutilating, macerating and smashing into the distilled water. Dilution and plating by spread plate method were carried out soon after sampling. Approximate 15 ml of plate count Agar (PCA), MacConkey Agar, Membrane Fecal Coliforms (mFC) Agar, Eosin Methylene Blue (EMB) Agar, Thiosulphate Citrate Bile Salt Sucrose (TCBS) Agar, Xylose Lysine Deoxycholate (XLD) Agar and Salmonella- Shigella Agar (SSA) has been melted and brought to 45°C was poured into the sterile Petri plates and different initiatives were taken for equal distribution of the media. After solidifying and inoculation, plates were inverted and placed in incubator at 37°C for 48 hours for evidence of growth. Total sixteen samples have been analyzed in terms of aerobic plate count (APC), enterobacteriaceae counts and Salmonella-Shigella (SS) counts. For the isolation of Vibrio spp. a pre-enrichment step was done. Pure isolates of resulting growth were identified using different biochemical tests for example Kligler’s Iron Agar (KIA) test, Indole production test, Methyl Red (MR) test, Voges-Proscauer (VP) test, Citrate utilization test, Motility Indole Urea (MIU) test, Carbohydrate fermentation test and salt tolerance test as described by Jolt et al..

Source of Information: World Journal of Agricultural Sciences 4 (S): 852-855, 2008, ISSN 1817-3047

Article Link (Online Journal): http://www.idosi.org/wjas/wjas4(s)/9.pdf

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

During this study it was observed that collected samples from departmental chain shops were more contaminated. The fact is that, all frozen products such as meat, fish and shrimps are stored together in departmental store; as a result cross contamination may occur. Not only that but also the product is stored for relatively long period until sold and sometimes storage condition cannot be ensured properly due to technological disruptio. Sanchita also found contamination carried over from the source of water, poor hygiene and sanitation condition of the processing premises. Therefore suggested that food processors should be educated on the adverse effect of contamination. Government should also come forth to implement the quality and precaution that are adopted in case of export products.

Knowledge Management: Journal