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Method Development and Validation for Estimation of Commercially Produced Sex Pheromones in Lure

SHANKAR MANDAL
Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

A M H RAHMAN
Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

M I R MAMUN
Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

MOHAMMAD SHOEB
Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

NILUFAR NAHAR
Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

A method was developed and validated for quantitation of methyl eugenol and cuelure, the active ingredients of two formulated sex pheromones (bio-pesticides) Mukti and Jhilik by gas chromatography. Technical grade methyl eugenol and cuelure (purity 95- 96%) were used as standards. Calibration curves of methyl eugenol and cuelure were linear with correlation coefficient (r²) 0.9966 & 0.9988, respectively. Ten replicate analyses were done for both methyl eugenol & cuelure and quantity of the pheromones in the two formulated products were found to be 0.55 ± 0.11 and 0.56 ± 0.09 g/lure (Mean ± SD), respectively. The stability of both pheromones in solution and their diffusion from lures under UV-Vis light was determined for a period of 21 days. The results showed that diffusion of the both pheromones from lure were very fast and about two-third diffused within 10 days after exposure. Methyl eugenol was quite stable in solution, whereas cuelure was found to degrade slowly, and about 15% cuelure degraded within 21 days. The recovery of methyl eugenol and cuelure from matrix were found to be 80.69 ± 3.14 and 78.48 ± 4.32% (mean ± SD), respectively, where limit of detection (LOD) & limit of quantification (LOQ) were found to be 97 & 80 ppm, and 290 & 240 ppm, respectively. The developed method is very simple and can be used for estimation of the two sex pheromones.

Keywords: Active ingredients, Lures, Sex pheromones

Location:

Start Date:

End Date:

Program Area: Pest Management

Non-commodity: Performance

Objectives:

An approach was taken to develop a suitable method for determination of active ingredients in the lures and to validate the developed method.

Methodology:

Sampling: Two formulated sex pheromones, methyl eugenol (Mukti) and cuelure (Jhilik) lures were collected from the Department of Agricultural Extension (DAE), Dhaka. Lures were made by soaking heavy cotton thread containing plastic clip with formulated methyl eugenol or cuelure and packed in tri-laminated pouch with aluminum foil. Blank lures (without active ingredients) were also collected from DAE to perform recovery experiments. Instruments: For Gas chromatography a Shimadzu 2025 GC fitted with flame ionization detector (FID) and quartz capillary column (HP-5; 30 m × 0.25 mm × 0.25 µm) were used. The injector and detector temperatures were set at 225?C and 275?C, respectively and nitrogen was used as carrier gas. Separations were performed at 110?C (1 min. hold) 15?C/min. to 220?C (6 min. hold) for methyl eugenol and 120?C (1 min. hold) 15?C/min. to 220?C (6 min. hold) for cuelure. Extraction and clean up: Lure was taken in a sealed ground joint graduated test tube. A mixture of n-hexane and acetone (1:1, 8 mL) was added to the test tube. The content was ultrasonicated for 30 second, rapped with parafilm and kept overnight (about 12 hrs) at room temperature. The extract was transferred in another sealed ground joint graduated test tube. The lure was reextracted for 4 hours with another 7 mL of same solvent mixture. The two extracts were combined together. A part of the extract was filtered through millipore membrane filters (0.45 µm pore size) and analyzed by GC-FID. Recovery experiment: Replicate recovery experiments of methyl eugenol and cuelure were done by spiking the blank matrix i.e lure and spiking level was 200 ppm in each case. The recovery of cuelure and methyl eugenol were found to be 78.48 ± 4.32 and 80.69 ± 3.14 % (mean ± SD), respectively. Stability and diffusion study: The stability and diffusion of both methyl eugenol and cuelure were studied in a period of 21 days under UV-Vis light. Both formulated lures were exposed under UV-Vis light and active ingredients were determined at 0, 3, 7, 15 and 21 days after exposure. To determine the stability of the pheromones, the extracts of both formulated lures were also exposed under UV-Vis light and active ingredients were determined at 0, 3, 7, 15 and 21 days after exposure.

Source of Information: Journal of Bangladesh Chemical Society, Vol. 25(2), 180-185, 2012

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Biopesticides such as sex pheromones can be analyzed by GC-FID very effectively. Both formulated products of cuelure and methyl eugenol have active ingredients to apply in integrated pest management (IPM) program effectively in a period of about 10 days. To reduce the loss of active ingredients attempt should taken for proper lure formulation, storage and packaging, so that sex pheromones will be an effective tool of pest controls system.

Knowledge Management: Journal