Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Management of Anthracnose and Blight Diseases of Houttuynia Cordata Thunb. with Fungicides

SHAMIM SHAMSI*
Department of Botany, University of Dhaka, Dhaka- 1000, Bangladesh

TRISHA SAHA
Department of Botany, University of Dhaka, Dhaka- 1000, Bangladesh

Abstract/ Executive Summary:

Two types of symptoms, namely anthracnose and blight were recorded on Houttuynia cordata Thunb. during the period of April to December 2013. The isolated fungi from the diseased plants were Alterneria alternata (Fr.) Keissler and Colletotrichum gloeosporioides (Penz.) Sacc. Five fungicides viz., Bavistin DF, Dithane M-45, Indofil, Salcox 50 WP and Tall 25 EC were evaluated against the pathogenic fungi at 100, 200, 300, 400 and 500 ppm concentrations. Tall 25 EC was found most efficient inhibitor of the test fungi followed by Bavistin, Indofil, Dithane M-45 and Salcox. In vivo treatment Tall 25 EC was found most effective in controlling the diseases.

Keywords: Anthracnose, Blight, Houttuynia cordata, Management, Fungicides

Location:

Start Date: 00-04-2013

End Date: 00-12-2013

Program Area: Pest Management

Commodity: Fungicide

Objectives:

So far, there is no report available regarding the control of the diseases caused by H. cordata. Present attempt was made to find out suitable control measures of the plant.

Methodology:

The leaves of H. cordata showing anthracnose and blight symptoms were collected from experimental field of Botanical Garden, Curzon Hall campus, Dhaka University, during the period of April, 2013 to December, 2013. The associated fungi were isolated from the samples following the “Tissue Planting” and “Blotter” method on PDA medium (CAB 1968). Identification of the isolates was determined following the standard literatures (Barnrett and Hunter 2000, Ellis 1971, 1976; Ellis and Ellis 1997, Sutton 1982). All the isolated fungi were tested for their pathogenic potentiality following “Detached leaf technique” (Azad and Shamsi 2011). The pathogenic fungi screened from “Detached leaf assay” were selected for net house experiment. Healthy plants of H. cordata were grown on 12 inch (diam.) earthen pot containing sterilized soil for 6 weeks. The overall experiment was conducted in net house. Five plants were transplanted in each pot. Three replications were made for each treatment. Five pots were provided for each replication. Test fungi were grown on PDA medium for 7-10 days. Spore suspension of each pathogenic fungus at the rate of 104 concentrations was sprayed on healthy plants using hand sprayer. Inoculated plants were covered by perforated polythene bags to avoid contamination and to maintain humidity. Symptoms were recorded and fungi were re-isolated to fulfill Koch’s postulate. Five fungicides with different active ingredients, viz., bavistin 50 WP, dithane M-45, indofil, salcox 50 WP and Tall 25 EC were collected from the Krishi Upokoron Biponi Kendro, Khamarbari, Farmgate, Dhaka. These fungicides at 100, 200, 300, 400 and 500 ppm concentrations were evaluated against the fungi. For each fungicide, a stock solution having the concentration of 10,000 ppm was prepared. The calculated amount of the stock solution of a fungicide was supplemented with sterilized PDA medium to get the above mentioned concentrations. The concentrations of fungicides are expressed in terms of its active ingredients. Twenty ml of the supplemented medium of a particular concentration was poured in a sterilized Petri plate and allowed to solidify. In the control set, required amount of sterile water instead of fungicide solution was added to the PDA medium. Five mm mycelial agar disc cut from the margin of actively growing culture of test fungi for each treatment was placed at the center of the plate. Three replications were maintained in each case. The inoculated plates were incubated at 25 ± 2?C. The radial growth the colonies was measured at the 5th day of inoculation. The fungitoxicity in terms of percentage inhibition of mycelial growth was calculated using the formula: Per cent inhibition= C-T/C X 100. where C = Average increase in mycelial growth in control plate and T = Average increase in mycelial growth in treatment plate. Among the five fungicides used in controlling in vitro growth of test fungi, Tall 25 EC showed the best result. Thus this fungicide was selected for in vivo test for controlling the test fungi in the net house at lowest concentration 100 ppm. Five plants were transplanted on each pot containing sterilized soil and allowed to grow for 6 weeks. Three replications were maintained for the experiment. Five pots were maintained for each replication. Suspension of Tall 25 EC at 100 ppm concentration was sprayed on healthy plants by hand sprayer. This suspension was made following the same way as it was made in the laboratory for in vitro test. Control plants were sprayed with distilled water. Then all the plants were covered by perforated polythene bag for 48 hours to slow the rate of evaporation of fungicide from plat surface. These pots were observed for one month for disease appearance under natural condition. The experiment was conducted in net house of Botanical garden, Curzon Hall, Campus, DU

Source of Information: Journal of Bangladesh Academy of Sciences, Vol. 39, No. 1, 83-90, 2015

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Effects of fungitoxicity in controlling diseases of Houttuynia cordata: Selected fungicide Tall 25 EC at 100 ppm concentration was sprayed over the plants in net house. After 15 days of application of fungicide the treated plants showed no infection. Moreover, plants treated with fungicides showed luxuriant growth as well as plant height and leaf size also increased. On the other hand control plants gradually become infected by the fungi showing symptoms. Plants become shorter compared to treated plants. Up to one month of observation, treated plants remained symptomless

Knowledge Management: Journal