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In Vitro Control of Associated Mycoflora of Oyster Mushroom Substrates by the Application of Fungicides

Pervez, Z.
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patukhali

Bhuiyan, M.K.A.
Department of Plant Pathology, BSMRAU, Gazipur1706, Bangladesh

Islam, M.S.
Department of Plant Pathology, Patuakhali Science and Technology University, Dumki, Patukhali

Abstract/ Executive Summary:

A laboratory trial has been conducted with Formalin, Bavistin and combination of Formalin and Bavistin at three different concentrations against identified associated mycoflora of Oyster mushroom substrates. The combination of Formalin and Bavistin at the highest concentration (500+75 ppm) was found to be the best in inhibiting the radial colony growth of all the identified fungi.

Keywords: Mycoflora, oyster mushroom, fungicide

Location: National Mushroom Development & Extension Centre, Sobhanbagh, Savar, Dhaka, Bangladesh

Start Date: 00-00-2006

End Date: 00-00-2007

Program Area: Variety and Species

Commodity: Mushroom

Objectives:

1. To find out the appropriate chemicals and their doses for controlling associated mycoflora with Oyster mushroom sbstrate.

Methodology:

A total of 50 spawn packets with colonized substrate of P. ostreatus were collected randomly at different growth stages from Mushroom Culture Center, Savar, Dhaka. Ten mycoflora including Aspergillus flavus, A. fumigatus, A. niger, A. nidulans, A. terreus, Penicillium citrinum, P. thiersii, Penicillium. sp., Rhizopus stolonifer and Trichoderma harzianum were isolated and identified as associated with P. ostreatus (Oyster mushroom) substrate. An attempt was made to control different mycoflora associated with substrate colonized Oyster mushroom with different fungicides in vitro. The experiments were conducted at National Mushroom Development & Extension Centre, Sobhanbagh, Savar, Dhaka, Bangladesh during 2006-2007. Laboratory evaluation of fungicides against associated mycoflora from different substrate of P. ostreatus - Three different concentrations namely 100, 250 and 500 ppm of Formalin, 25, 50 and 75 ppm of Bavistin and 100 +25, 250 + 50 and 500 + 75 ppm of Formalin + Bavistin were tested in vitro to evaluate their effect on the radial growth of isolated fungi. Colony of the isolated fungi from selected 50 packets of wheat grain colonized substrate of P. ostreatus were cultured on PDA. Each of the isolated fungus was treated against the selected three chemicals at three different concentrations separately following poison food technique. The required amounts of fungicides were mixed with melted PDA. Control was taken into the design of experimentation by keeping untreated colony on PDA medium. Efficiency of chemicals at different concentrations were evaluated on the basis of percent inhibition of the radial colony growth of the isolated fungi, and computed as a proportional measurement of colony diameter on control plate expressing in percentage, % Inhibition = [(X - Y)/X ] x 100, Where, X= radial growth (mm) of control plates, Y = radial growth (mm) of fungicide treated plates.

Source of Information: BANGLADESH RESEARCH PUBLICATIONS JOURNAL; ISSN: 1998-2003, Volume: 2, Issue: 4, Page: 737-741, July - August, 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The results of the present study prevailed that both the fungicides Formalin and Bavistin are effective against the associated molds of Oyster mushroom but when these fungicides are used in combination were highly effective.

Knowledge Management: Journal