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Evaluation of Some Rapeseed Mutants Based on Morpho-physiological, Biochemical and Yield Attributes

M. A. Uddin
Dept. of Crop Botany,Bangladesh Agricultural University, Mymensingh

M. Z. Rahman
Dept. Agricultural Extension Education, Bangladesh Agricultural University, Mymensingh

K. M. Rahman
Dept. of Environmental Science,Bangladesh Agricultural University, Mymensingh

F. Sultana
Dept. of Crop Botany,Bangladesh Agricultural University, Mymensingh

M. A. Ullah
Bangladesh Institute of Nuclear Agriculture, Mymensingh

Abstract/ Executive Summary:

An experiment was conducted at the field laboratory of Bangladesh Institute of Nuclear Agriculture, Mymensingh, to investigate morpho-physiological characters, yield attributes and seed yield in seven advanced mustard mutants viz., RM 01,RM 02,RM 03,RM 04,RM 05,RM 10 and RM11along with a cultivar BINAsarisa-4.The experiment was laid out in a Randomized Complete Block Design with three replications. High yielding genotypes showed superiority in leaf chlorophyll content, photosynthesis, total sugar content and nitrate reductase activity. RM 05 produced higher number of siliqua plant^-1 resulting higher seed yield. In contrast, the mutants RM 01 and RM 10 produced the lower number of siliqua plant^-1and also showed the lower seed yield with being the lowest in RM01.Among the mutants/cultivars, RM 05 produced the highest seed yield due to morpho-physiological superiority than the others.

Keywords: Attributes, Biochemical, Evaluation, Mustard, Mutants

Location: Bangladesh Institute of Nuclear Agriculture, Mymensingh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mustard

Objectives:

1. The present research work has been designed to study different growth parameters and other morphophysiological characteristics responsible for higher biological yield as well as their interrelation to the grain yield.

Methodology:

The experiment was carried out at the Field laboratory, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensing. The experimental field was medium high land belonging to the Sonatola Soil Series of Grey Floodplain soil under the agroecological zone of Old Bahmaputra Flood plain (AEZ-9).The soil was silty loam. Treatment - Seven advanced mutant lines along with one mustard variety (BINAsarisa-4) were collected from plant Breeding Division, BINA, Mymensing and used as treatment in the present study. The characters of advanced mutant lines are still unknown. The average yield potential of BINAsarisa-4 is about 2.0 t ha-¹.The land of the experimental site was first opened with power tiller. After ploughing and laddering all the stubbles and uprooted weeds were removed and the land was made ready. Experimental design and lay-out - The experiment was laid out in a Randomized Complete Block Design with 3 replications. The size of the unit plot was 4.0×2.5m. Distance between block to block and plot to plot were1.0 and05 meter, respectively. Plant to plant and row to row distances were maintained as 5-8 cm and 30 cm, respectively. Urea, TSP, MP, gypsum and borax were used. Weeding was done once at 20 DAS only. Two irrigations were applied. At flowering, few plants were affected by aphid. To control aphid, Malathion 57 EC was sprayed@ 25 L ha^-1 in the afternoon by using a sprayer. Crop sampling and data collection - The date of harvest was determined when 85-90% of the siliqua became brown colour. The maturity dates were different among the varieties. The plots were harvested separately and tagged and brought to the threshing floor and dried in the sun. The seed were threshed by mild pounding with stick. Data Collection - The following data were collected by using the standard methods: 1. Days to maturity, 2. Branches/plant(no), 3.Chlorophyll, 4.Photosynthesis, 5.Nitrate Reductase, and 6.Total sugar. The collected data were analyzed statistically following the analysis of variance(ANOVA) technique and the mean differences were adjusted with Duncuns Multiple Range Test(DMRT) using the statistical computer package program, MSTAT-C

Source of Information: J. Environ. Sci. & Natural Resources, 5(2): 281 - 285 , 2012; ISSN 1999-7361

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

High yielding mutants produced higher number of branches plant^-1, higher chlophyll, phosynthates, total sugar content in leaf, nitrate reductase activity than low yielding ones. Days to maturity was not significantly different among the mutants. Reproductive efficiency (RE) had positive effect with yield. The high yielding mutants showed higher RE (average 66.1%)than low yielding ones (average 55.25).Siliqua number, the most important yield attribute, showed significant differences among the variety. Results revealed that high yielding nutants produced higher numbers of pods plant^-1. RM 05 produced the highest number of pods plant^-1(55.4) whilst RM 01 produced the lowest (40.1).High yielding mutants also produced longer siliqua and greater number of seed siliqua^-1 but there had no relation of 1000-seed weight with the yield.

Knowledge Management: Journal