Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Influence of Breed, Age and Collection Interval on Semen Quality of Ai Dairy Bulls in Bangladesh

KU Ahmed
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202,Bangladesh.

MMU Bhuiyan
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202,Bangladesh.

MM Hossain
Department of Anatomy, Histology and Physiology, Sher-e-Bangla Agricultural University, Dhaka

Z. Rahman
Deapartment of Animal Science, Faculty of Animal Husbandry, Bangladesh Agricultural University, Mymensingh-2202,Bangladesh

MKU Talukder
Artificial Insemination Laboratory, Central Cattle Breeding Station (CCBS), Savar, Dhaka.

MR Islam
Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202,Bangladesh.

Abstract/ Executive Summary:

The study was conducted at the AI laboratory of Central Cattle Breeding Station (CCBS), Savar, Dhaka. The objectives of the present investigation were to determine the influence of breed, age and collection interval on quality of bull semen (Local, Friesian cross, Friesian pure and Sahiwal pure). Experiment 1, determine the age influence, nine Friesian cross bulls of three different age groups (24-48, 49-96 and 97-120 months). Significantly higher volume of semen was obtained from local (6.3±0. 5 ml) and Friesian Cross bulls (5.8±0.3) than that of Friesian counterpart (4.3±0.3 ml) (P<0.05). Significantly higher sperm concentration and total spermatozoa were obtained in Local (1752.0±181.6 x 106 /ml and 10952.7±1315.5 x 106/ejaculate, respectively) and Sahiwal bulls (1528.2±114.9 x 106 /ml and 7480.8±648.7x 106 /ejaculate, respectively) than those of Friesian counterpart ( 1043.5±93.2 x 106 /ml and 4611.4±662.4 x 106 /ejaculate, respectively) (P<0.05). The fresh semen motility and post-thaw motility varied from 56.6±2.3 to 60.5±1.5% and 48.8±1.6 to53.3±1.4%,respectively (P>0.05). Experiment 2, determine the influence of collection interval, three Friesian Cross bulls at three days and seven days interval were collected. Semen volume ranged from 5.8±0.3 to 6.4±0.7 ml, fresh semen motility ranged from 60.0±2.2 to 61.1±1.8 %, sperm concentration ranged from 1199.3±106.0 to 1290.2±88.2 x 106/ml, total spermatozoa ranged from 7201.8±971.5 to 8026.7±884.1 x106/ejaculate and post-thaw motility ranged from 49.4±1.0 to 52.7±1.4 %. The difference in semen parameters did not differ significantly among age groups of bulls (P>0.05). Significantly higher semen volume (5.1±0.4 ml), fresh semen motility (62.2±2.0%) and total spermatozoa (8543.8±885. 4 x10 /ejaculate) were obtained when the bulls were collected at 7 days intervals than that of 3 days counterparts (3.0±0. 4 ml, 55. 0±1.8%, 4050.1±689.2x106/ejaculate, respectively; P<0.05). However, sperm concentration (1347.5±120.2 to 1679.0±126.3 x106/ml), post-thaw motility (47.7±1.4 to 50.0±1.4%), proportion of normal spermatozoa with respect to acrosome, midpiece and tail in fresh (89. 4±0.8 to 90.2±0.7%) and post- thaw semen (89.2±1.2 to 92.1±0.4% ) did not differ significantly between two types of collection intervals (P>0.05) . In conclusions, first ejaculate of local bulls is better than Friesian bulls with respect to semen volume, sperm concentration and total spermatozoa per ejaculate. Age of Friesian cross bulls does not influence the quality of semen and Friesian cross bulls collected at 7 days interval is better with respect to semen volume, fresh motility and total spermatozoa than that at 3 days interval.

Keywords: Age, Breeds of bull, Collection interval, Semen quality

Location: AI laboratory of Central Cattle Breeding Station (CCBS), Savar, Dhaka

Start Date: 00-07-2011

End Date: 00-11-2011

Program Area: Animal Health and Management

Commodity: Cattle

Objectives:

1. To determine the influence of breed, age and collection interval on different parameters of bull semen used for AI in Bangladesh.

Methodology:

Experimental Animals - In this study, 18 dairy bulls of four breeds namely Local (nondescript indigenous) (n=3) , Friesian cross with Local (n=9), Friesian pure (n=3) and Sahiwal pure (n=3) were used. According to the bull register, age of the bulls ranged from 24 to 120 months, body weight ranged from 390 to 1070 kg, BCS ranged from 4.0 to 4. 5 and scrotal circumference ranged from 35 to 39 cm. Feeding of Bulls - The bulls were provided feed at the rate of 1. 5 to 2.0% of the total body weight on drymatter basis. Total required feed included 25% concentrates and 75% roughages. The concentrate feed included wheat bran, maize, rice polish, khesari bran, soyameal, gram, dicalcium-phosphate and table salt. The roughages included green grass andstraw. Water was provided adlibitum. A general management schedule for deworming and vaccination was in practice. Semen Collection - Usually semen collection was performed once a week. Two ejaculates were collected from each bull during each collection session and only the first ejaculate was used in this experiment. Collection was always performed in the morning between 7 and 9 AM. To determine the influence of collection interval, 3 consecutive collections from 3 selected bulls were done 3 days apart. Semen was collected using artificial vagina (AV) method following standard procedure at homo sexual mount. The bulls were allowed at least two false mounts before collection of semen. Immediately after collection, the collecting tube was detached from the AV, taken to the laboratory and p laced at 37⁰C in water bath until evaluation and processing of semen. Evaluation of Semen Individual fresh ejaculates were evaluated for volume, sperm motility, sperm concentration, total spermatozoa per ejaculate and proportion of spermatozoa with normal acrosome, midpiece and tail. The volume of fresh semen was recorded from the graduated mark of the semen collecting tube. To evaluate the sperm motility, a small drop (10μl) of semen was placed on a clean pre-warmed glass slide and covered with a cover slip. The motility was determined by eye estimation observing the proportion of spermatozoa actively moving forward at medium magnification (200x) and expressed as percentage. Sperm concentration was measured by spectrophotometer following the manufacturer’s instruction and expressed as million per ml. Total spermatozoa per ejaculate was measured by multiplication of sperm concentration with volume of ejaculate andexpressed as million per ejaculate. The proportion of normal spermatozoa with respect to acrosome, midpiece and tail was evaluated in buffered formol saline-fixed semen. The buffered formol saline was prepared. A drop (10μl) of formol saline-fixed semen was placed on a clean g lass slide with a cover slip and the edges were soaked with tissue paper to remove excess fluid. The slide was then held for five minutes to allow spermatozoa to settle down and then examined under a microscope that was equipped with differential interference contrast (DIC) optics (1000x; Olympus®, Bx51 Olympus Optical Co. Ltd.,Tokyo, Japan). Preservation of Semen - The egg yolk-tris-fructose-citric acid-glycerol extender was used as diluent for preservation of semen as frozen condition. Briefly, a stock solution was prepared by dissolving tris (297.6 mmol), fructose (82.6 mmol), citric acid (105.3 mmol), penicillin G sodium ( 1000 I.U/ml) and streptomycin sulphate (1 mg/ml) in glass distilled water . Fresh yolk was added with the buffer at a concentration of 20% (v/v). The volume of extender was divided into two equal parts. Thereafter, glycerol was added to one part of the extender at two times of the desired concentration (12.8%). The other part of the diluents was used to make the initial dilution of semen that contained two times of the desired concentration of spermatozoa. The initial dilution of semen was made at +37⁰ C. Theequal parts of initially diluted semen and double concentration glycerol- containing extender were mixed together at four steps during a 2 to 3 hours cooling process, the dilution steps were at +18, +12, +8 and +40C. Finally the diluted semen contained 6.4% (v/v) glycerol and 30x10 motile spermatozoa per 0.25 ml French straw (one insemination 6 dose). Individual insemination doses were sucked in 0. 25 ml French straws and the open ends of the straws were sealed by using automatic filling and sealing machine. After loading, the straws were left for equilibration at +4⁰C for an hour . The cooling and equilibration operation were done in a cold handling cabinet. After equilibration, the freezing operation was conducted in semen freezer (HN, Mini tub, Germany) to cool the semen from +4 to - 140⁰C during a period of 20-30 min and then the straws were directly plunged into liquid nitrogen (-196⁰C). Only semen that showed =50% post-thaw motility was preserved for AI. Thawing of Semen - To check the post-thaw motility and taking sample for formol-saline fixation, the frozen semen straws were thawed at +370C warm water for 12 sec at 24 hours postfreezing. Statistical Analysis - All data were presented as mean ± standard error of mean (SE) . One way analysis of variance ( ANOVA) followed by least significant difference (LSD) was done to find out significant differences among the different levels of breed and age variables. Pair t-test was do ne to find out the significant differences between the two categories of collection interval of semen. All the statistical analyses were do ne using SPSS 17.0. The difference between groups was regarded as significant when the P value was less than 0.05 (P<0. 05).

Source of Information: BANGLADESH RESEARCH PUBLICATIONS JOURNAL; ISSN: 1998-2003, Volume: 10, Issue: 3, Page: 275-282, November - December, 2014

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The first ejaculate of local bulls is better than Friesian bulls with respect to semen volume, sperm concentration and total spermatozoa per ejaculate. Age (24-120 months) of Friesian cross bulls may not influence the quality of semen with respect to volume, fresh motility, sperm concentration, total spermatozoa and post-thaw motility. Friesian cross bulls collected at 7 days interval might be better with respect to semen volume, fresh semen motility and total spermatozoa than that at 3 days interval.

Knowledge Management: Journal