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Fruit Production and Biochemical Aspects of Seeds of Euryale Ferox Salisb. Under Ex-situ Conditions

MD. ALMUJADDADE ALFASANE
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

MONIRUZZAMAN KHONDKER, Z. N.
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

TAHMIDA BEGUM,
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

LAILA ARJUMAND BANU
BCSIR Laboratories, Dhaka, Bangladesh.

MD. MAHBUBAR RAHMAN
BCSIR Laboratories, Dhaka, Bangladesh.

UMMA FATEMA SHAHJADEE
BCSIR Laboratories, Dhaka, Bangladesh.

Abstract/ Executive Summary:

Ex-situ culture studies of Euryale ferox Salisb. using fresh mature seeds were carried out in an arboretum. Production of fresh fruit has been estimated to be 3.05 t/ha. Biochemical analysis of the seeds revealed 61% carbohydrate, 15.6% protein, 12.1% moisture, 7.6% fibre, 1.8% ash and 1.35% fat. The seeds were found to contain 12 amino acids, which are histidine, leucine, isoleucine, glutamic acid, lysine, tyrosine, valine, aspartic, threonine, alanine, methionine and arginine.

Keywords: Euryale ferox, Ex-situ culture, Fruit production, Seeds biochemical content

Location:

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Makhna

Objectives:

In the present study, an ex-situ culture of E. ferox for the production of Makhna fruits, and also biochemical contents of seeds produced have been investigated.

Methodology:

Euryale ferox Salisb. was propagated via seeds. Fresh and mature fruits of E. ferox were collected on April 26, 2002 from a local market of Dhaka metropolis where the item is regularly sold. After the fruits were brought to the laboratory it was incised and the seeds were taken out and set for germination. The germination was carried out in an earthen bin. It had a diameter of 77 cm and a depth of 40 cm and buried on the ground in such a way that the rim remains 6 cm above. Nearly one third of the volume of the bin was filled with 10 kg loam mixed with composted cow dung (1 : 1) and then it was filled with tap water. At the end of April 2002, a perforated poly-bag containing 30 healthy seeds of E. ferox were sown in the soft and submerged mud of the bin. At the time of germination following environmental factors such as air temp. (31ºC), water temp. (33ºC), pH (7.17), TDS (302 mg/l), conductivity of water (664 µS/cm, alkalinity (3.15 meq/l), and DO (12.20 mg/l) were recorded. After a period of three - four weeks, 14 seeds germinated. The seedlings were transferred after a month to another smaller shallow concrete tank situated at the Botanical garden, Department of Botany, University of Dhaka. In June, the plantlets grew up to 30 - 40 cm in height, and the plantlets were transferred to a concrete cistern of 90 cm wide and 200 cm long, where the bottom was filled with composted cow dung mixed with loam up to 30 cm height. Immediately after transplantation tap water was added in the cistern in such a way that only the leaves of the plants remained afloat. The water surface was adjusted every day as per growth of plants. Fresh seeds obtained from 36 mature fruits of E. ferox were crushed in a mortar with a pestle. The crushed material was used to determine the amount of protein, ash, fat, moisture, fibre and carbohydrate content (Nat. Inst. Nutrn. 1976). Moisture content was determined with the help of a moisture meter (IB-30, Brand Chyo) and protein was determined by Microkjeldhal method. The ash content was determined in a Muffle furnace (Carbolite RHF-1600). For analysis of amino acids, seeds were crushed and a fine paste was made by mortar and pestle and transferred in 250 ml round bottom flask placed in a heating mantle at 110°C for 24 h with 6N HCl. The solution obtained was kept in an evaporating dish to evaporate HCl on water bath. It was then filtered to 25 ml volumetric flask through Whatman No. 9 filter paper and columned with 0.1N HCl. The solution was run through an Amino acid analyser (Schimadzu, Japan). The analyser showed the standard curve for standard solution and another curve for sample solution. By comparing the two curves the amount of amino acids was calculated.

Source of Information: Bangladesh J. Bot. 37(2): 179-181, 2008 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Production of fresh fruit has been estimated to be 3.05 t/ha. Biochemical analysis of the seeds revealed 61% carbohydrate, 15.6% protein, 12.1% moisture, 7.6% fibre, 1.8% ash and 1.35% fat. The seeds were found to contain 12 amino acids, which are histidine, leucine, isoleucine, glutamic acid, lysine, tyrosine, valine, aspartic, threonine, alanine, methionine and arginine.

Knowledge Management: Journal