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Essential Oil in Roots of Vetiveria Zizanioides (l.) Nash Ex Small from Bangladesh

MD. NAZRUL ISLAM BHUIYAN*
BCSIR Laboratories, P.O.- Chittagong Cantonment, Chittagong-4220, Bangladesh

JASIM UDDIN CHOWDHURY
BCSIR Laboratories, P.O.- Chittagong Cantonment, Chittagong-4220, Bangladesh

JARIPA BEGUM
BCSIR Laboratories, P.O.- Chittagong Cantonment, Chittagong-4220, Bangladesh

Abstract/ Executive Summary:

Essential oil from roots of Vetiveria zizanioides (L.) Nash ex Small from Bangladesh was examined by gas chromatography mass spectrometry (GC-MS). Fourteen compounds were identified in root oil. The compositions of oil varied qualitatively and quantitatively.

Keywords: Vetiveria zizanioides, GC-MS analysis, Essential oil

Location: BCSIR Laboratory, Chittagong

Start Date: 00-03-2007

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

The present investigation was undertaken to determine the chemical composition of root oil of V. zizanioides grown in Bangladesh.

Methodology:

The plant material of V. zizanioides was collected from the medicinal garden of BCSIR Laboratory, Chittagong during March 2007. Samples of root were harvested from healthy, well-grown plants. Freshly harvested root samples (500 g) were grounded in a blender. The material was subjected to hydrodistillation using a modified Clevenger-type glass apparatus for 4 h for isolation of oils. The oil samples were stored at 0°C in air-tight containers after drying them over anhydrous sodium sulfate, filtered and concentrated under reduced pressure at room temperature to obtain the essential oil for GC-MS analysis. The essential oil from root of V. zizanioides was analyzed by GC-MS electron impact ionization (EI) method on GC-17A gas chromatograph (Shimadzu) coupled to a GC-MS QP 5050A Mass Spectrometer (Shimadzu); fused silica capillary column (30 m × 2.5 mm; 0.25 µm film thickness), coated with DB-5 (J&W); column temperature 100^oC (2 min) to 250^oC at the rate of 3^oC/min; carrier gas, helium at constant pressure of 90 Kpa. Acquisition parameters full scan; scan range 40 - 350 amu. Compound identification was done by comparing the NIST (National Institute of Standards and Technology) library data of the peaks with those reported in literature, mass spectra of the peaks with literature data. Percentage composition was computed from GC peak areas on BD-5 column without applying correction factors.

Source of Information: Bangladesh J. Bot. 37(2): 213-215, 2008 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Fourteen compounds were identified accounting for 100% of the total oils. Result obtained indicated that V. zizanioides, growing widely in Bangladesh, may be utilized as a source for the isolation of these two compounds as those are in high concentration in root oil, which has potential antimicrobial activities

Knowledge Management: Journal