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Influence of Indole-3-butyric Acid (iba) on Sprouting and Rooting Potential of Bougainvillea Spectabilis Cuttings

H. Mehraj
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

A.F.M. Jamal Uddin
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

S. Shahrin
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

T. Taufique
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

I.H. Shiam
Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Abstract/ Executive Summary:

An experiment was carried out at the Department of Horticulture at Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for the investigation of the performance of different concentration of Indole-3-Butyric Acid (IBA) for sprouting and rooting potential of Bougainvillea spectabilis stem cutting during the period from May 2013 to August 2013. Five treatments viz. T₀: Control; T₁: IBA in dust form; T₂: IBA 500-ppm; T₃: IBA 1000-ppm and T₄: IBA 2000-ppm were exploited on the experiment with five replication. In case of T₂, T₃ and T₄ cuttings were soaked for 24 hours on the respective solutions. From the experiment it was found that IBA play an important role for successful asexual propagation of bougainvillea through cutting. Better performance was found when cut stems were soaked for 24 hours before the establishment of stem cutting. But performance was inhibited by increasing concentrations of IBA in terms of all of the features on which data had taken. During the experimental period, data was taken on different attributes and T₃ (1000- ppm) was performed as the best among the treatments which as followings: days to first rooting (4.0), days to first sprout bud initiation (5.3), number of sprout bud/cutting (3.6), number of leaves/cutting (35.2), length of sprout (15.0 cm), number of branches/cutting (4.7), number of root/cutting (64.2), number of sub root/cutting (25.8), longest root length (33.2 cm), longest root diameter (0.51 mm), rooting % (100%), survival % of rooted cuttings (100%). Though 100% rooting was also found in 500-ppm and 2000-pmm but only 1000-ppm was resulted 100% survival of rooted cuttings.

Keywords: Bougainvillea, Indole-3-Butyric Acid (IBA), Sprouting and Rooting

Location: Department of Horticulture at Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Start Date: 00-05-2013

End Date: 00-08-2013

Program Area: Variety and Species

Commodity: Plant

Objectives:

1. To search the proper IBA treatment for the asexual vegetative propagation by cutting of Bougainvillea.

Methodology:

An experiment was conducted at the Horticultural farm, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh to investigate the influence of different concentration of IBA on sprouting and rooting potential of bougainvillea cutting during the period from May 2013 to August 2013. Five treatments viz. T0: Control; T₁: IBA in dust form; T₂: IBA 500- ppm; T₃: IBA 1000-ppm and T₄: IBA 2000-ppm were exploited on the experiment with five replication. One and half year mature stem were selected for stem cutting. 15 cm stem were cut for the experiment with the help of a sharp knife to avoid injury of the cuttings. Slanting cut was made at base in order to provide more nutrient accumulating area also for receiving the maximum number of roots and horizontal cut were made on the top of the cuttings. Each treatment contained 10 cutting on each replication. Cuttings were soaked on 500-ppm, 1000-ppm, and 2000-ppm IBA solution for 24 hours and the IBA dust were attached to the cuttings just before the establishment of the cuttings. Fungicides were sprayed on the whole portion of the cuttings before establishment on soil. Data were collected from all of the cutting and then mean was calculated. Five cuttings were placed on the transparent bottle in water to observe the days to first root initiation. Data were taken on days to first rooting (visual observation), days to first sprout bud initiation (visual observation), number of sprout/cutting, number of leaves/cutting, length of sprout (cm), number of branches/cutting, number of root/cutting, number of sub root/cutting, longest root length (cm), longest root diameter (mm), rooting %, survival % of rooted cuttings. Data for length of sprout (cm), number of branches/cutting, number of root/cutting, number of sub root/cutting, longest root length (cm), longest root diameter (mm) was taken at 45 days after establishment of stem cuttings. Rooted cuttings were uprooted and planted on to poly bag to observe the survival percentage. Collected data were statistically analyzed by using MSTAT-C computer package program then mean was calculated and analysis of variance for each of treatment was represented by F-test (Variance Ratio). Differences between treatments were evaluated by Least Significance Difference (LSD) Test at 5% level of significance.

Source of Information: BANGLADESH RESEARCH PUBLICATIONS JOURNAL; ISSN: 1998-2003, Volume: 9, Issue: 1, Page: 44-49, September - October, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Plants can be transplanted when they have rooted, from the above result and discussion it can be concluded soaking the cut stem on 1000-ppm IBA solution for 24 hours before the establishment of the stem cutting can be used for the successful asexual propagation of bougainvillea through stem cutting as cuttings of this treatment sprouted, rooted and survive more percentage of rooted cutting along with more number of root, sprout bud, maximum root length and diameter.

Knowledge Management: Journal