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Development of Salt Tolerant Rice Variety Through Anther Culture

Enamul Hoque
Chief Scientific Officer & Head of the Division (Ad. Ch.)
Biotechnology Division, BRRI Head Office, Gazipur.

Shahanaz Sultana
Senior Scientific Officer
Biotechnology Division, BRRI Head Office, Gazipur.

Nilufar Yasmin Shaikh
Senior Scientific Officer
Biotechnology Division, BRRI Head Office, Gazipur.

Abstract/ Executive Summary:

Soil salinity is one of the major environmental stresses affecting the rice growth and reduces the grain yield significantly. In vitro production of double haploid plants through anther culture provides an efficient system for rapid production of homozygous lines. Therefore, attempt has been undertaken for developing suitable salt tolerant rice variety through anther culture. Boots were collected at appropriate stage from F₁ plants and pre-incubated at 8ºC for 8 days. The panicles were then cut into small pieces and surface sterilized by 70% ethanol. For callus induction, anthers were incubated on two different media (KE and M10) and kept in an incubator under dark condition at 25ºC. Induced calli were then transferred into regeneration medium (MS medium containing 1.0 mg/l NAA+ 1.0 mg/l Kinetin) for regeneration. Data were taken on number of anther plated, number of calli produced, number of albino and green plants regenerated. A total of 62657 hybrid anthers of 27 F₁ populations were plated in KE and M10 media for callus induction. A total of 330 calli were obtained from KE medium and 10 calli were obtained from M10 medium. The highest numbers (73) of calli were obtained from hybrid anthers of BRRI dhan29/MR219 in KE medium. However, nine green plants were regenerated from hybrid anther of BRRI dhan29/FL478 and three plants from MR219/IRBB60, respectively. After hardening, green plants were transferred into the earthen pots and seeds were harvested at maturity. Harvested seeds will be used for further evaluation and screened for tolerance to salinity.

Keywords: Anther-culture, Callus, Regeneration, Rice.

Location: Biotechnology Division, BRRI Head Office, Gazipur 1701.

Start Date: 01-07-2013

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To develop salt tolerant rice varieties.

Methodology:

Twenty seven F₁s populations were grown in the net house under optimum management. Boots with appropriate stage (uninucliate/mid uninucleate at reduction division stages) were collected from F₁ plants. First the boots were pre-incubated in a refrigerator at 8ºC for 8 days. The panicles were then taken from the boots and cut into small pieces. They were then surface sterilized by 70% ethanol for 2-3 minutes and then placed onto the sterile filter paper in Petri dishes to remove excess alcohol. For callus induction, anthers were incubated into two different media (KA an M10) media and kept in an incubator under dark condition at 25ºC until cullus initiation. Calli were then transferred into regeneration medium (MS medium containing 1.0 mg/l NAA+ 1.0 mg/l Kn), and culture were then incubated in a culture room under cool white Fluorescent lamps (approximately 1000 lux, under 25ºC) for regeneration., Data were taken on number of anther plated, number of callus produced, number of green and albino plant regenerated.

Cross	Anther plated	No of Callus	Plant regeneration
BR29/MR219	5043	73	2 albino
BR29/IRBB60	3057	6
MR219/FL378	485	-
BR29/IRBB65	255	10
BR29/FL478	4508	50	9 green plants
MR219/FL478	3326	13	1 albino plant
MR219/IRBB60	249	15	3 green plants
BR29/Lalmota//BR11/BR11	439	10	1 albino
BR11/Lalzarmota//BR29/29	7101	9	1 albino
MR219/BR29	1481	4
BR11/FL378	1607	5
Kanaklata/MR219	1176	-
BR16/Kanaklata	3537	31	1 albino
Kanaklata/BR16	2629	12
BR29/BR40	3990	10
BR29/BR47	120	-
BR28/BR40	190	-
MR219/Kanaklata	1062	33
**O.nivara/BR11**	1648	9
BR4909-R1-R2/BR40	10454	4
BR 29/kanaklata	4948	13
BR29/BR16	2602	7
BR41/Sadiq	344	-
BR6157-R1-1/BR6158RWBC2-1-2-1-1-1	692	8
BR29/BR49	218	3
BR6157-R1-1/BR29	390	2	2 albino
BR4909-R1-1/BR47	1106	3
	62657	330	12 green plants

Source of Information: Proceedings of the BRRI Annual Internal Review for 2013-2014

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

A total of 62657 hybrid anthers of 27 F₁ populations were plated in KE and M10 media for callus induction. A total of 330 calli were obtained from KE medium and 10 calli were obtained from M10 medium. The highest numbers (73) of calli were obtained from hybrid anthers of BRRI dhan29/MR219 in KE medium. Only nine green plants were regenerated from hybrid anther of BRRI dhan29/FL478 and three plants from MR219/IRBB60. After hardening green plantlets were transferred into the earthen pots and kept there until maturity.

Knowledge Management: Report/Proceedings