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Effects of Growth Regulators on Root Tip Cells of Onion

MD. MOSLEH UD-DEEN
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.

GOLAM KABIR
Department of Crop Science & Technology, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

Cell size and mitotic index were decreased with increasing concentrations of growth regulators such as GA3, uniconazole, chlorocholine chloride (CCC) and 2,3,6-TBA while nuclear volume, interphase chromosome volume and abnormalities were found to increase during division of onion root tip cells. Formation of chromosome fragments, bridges, laggards, single and multiple chromatid bridges, irregular distribution and unequal separation of chromosomes were visualized as the main effects due to the application of these growth regulators.

Keywords: Growth regulators, Onion, Root tip cell, Chromosome abnormalities

Location:

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Onion

Objectives:

The aim of this work was to study the comparative effects of four growth regulators at different concentrations on cell division using onion root tip cell.

Methodology:

A local variety of onion called “Taherpuri” was used as experimental materials and four growth regulators, namely Gibberellic acid-GA3 (growth promoter), uniconazole (growth retardant), chlorocholine chloride (CCC, growth retardant) and 2,3,6-trichlorobenzoic acid-2,3,6 (TBA, growth retardant and herbicidal) were used. Each growth substance was applied at five different concentrations such as 100, 150, 200, 250 and 300 ppm. During the treatment the bulbs of onion were placed on the mouth of each specimen tubes until the roots grew up to 1.0 to 1.5 cm in length. One tube was filled with water as control. Root tips were collected and fixed in 1:3 acetic acid-alcohol for 48 hours and stored in 70% ethanol in a refrigerator. Chromosomes were stained with 0.5% haematoxylin following the method of Haque et al. (1976). The nuclear volume (NV) was calculated using the formula as suggested by Nayar et al. (1971). The recorded data were analyzed statistically following MSTAT-C package program. The comparative effects of four growth regulators on cell (differentiated) size, interphase chromosome volume (ICV), mitotic index and chromosome abnormalities were recorded.

Source of Information: Bangladesh J. Bot. 38(1): 99-102, 2009 (June)

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Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present findings revealed that the chromosomal abnormalities were found to increase with increased doses of all growth regulators. Similar results were reported by Wuu and Grant (1967) in the root tip cells of the C1 generation in barley seeds treated with B-995. It is evident that growth regulators have different kinds of impact on cells even at low doses. Therefore, the recommended dose for each growth regulators must be applied.

Knowledge Management: Journal