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Effects of Waterlogging on Ion Accumulation and Sugar, Protein and Proline Contents in Corchorus Capsularis L.

Dilruba Parvin
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

J.L. Karmoker
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

Waterlogging decreased accumulation of K+, Ca²⁺, Mg²⁺, NO₃^- and PO₄ ^3- but increased that of Na+ and Fe²⁺ⁱn the root, stem and leaves of jute (Corchorus capsularis L.). It increased the accumulation of sugar and proline contents but decreased that of protein as well as dry weight. The effect of waterlogging on ion transport, accumulation of metabolites and dry weight was discussed.

Keywords: Waterlogging, Jute, Cation, Anion, Sugar, Protein, Proline

Location: Bangladesh Jute Research Institute, Dhaka.

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Jute

Objectives:

To investigate the effects of waterlogging on ion transport, metabolism and growth activities of Corchorus capsularis L .cv. D I54.

Methodology:

Seeds of jute (Corchorus capsularis L. cv. DI54) was collected through the courtesy of Bangladesh Jute Research Institute, Dhaka. Plants were grown in water culture in half-strength Hoagland solution. Seeds were germinated in purified quartz sand contained in earthern pot lined with polythene and seedlings were grown up to 15-day-old stage. Seedlings were further grown for another 21-day-old stage under anaerobic (treatment) or aerobic condition in a black painted plastic buckets with a lid having ten holes (3 cm in diameter) containing 5 litre of nutrient solution. One seedling was inserted in each hole and the seedling was plugged with spongy foam for support. Solution was replaced after every 48 hrs. In control, solution was aerated continuously by passing air with an air compressor and in treatment anaerobic condition was imposed by supplying nitrogen gas. Root, stem and leaf were collected in triplicate from three plants following waterlogging treatment. K⁺, Na⁺ and NO₃ ^- were extracted from dry tissue by boiling in a water bath K + and Na+ in plant tissue were analyzed by flame photometer and NO₃- was measured. For the determination of Ca²⁺, Mg²⁺, Fe²⁺ and PO₄^3-, dry plant tissue was extracted by digesting in a mixture of HNO₃- and HCl₃O₄ (4 : 1) in a sand bath. Ca²⁺, Mg²⁺, Fe²⁺ were measured in the digest by atomic absorption spectrophotometer and PO₄^3- was measured. Reducing and total sugars were extracted by boiling fresh tissue in 80% ethanol. Reducing sugar was determined by Somogyi-Nelson method. Total sugar was determined by phenol-H₂SO₄ method. Protein was extracted by homogeniging 1 g fresh tissue in chilled 2 mM KH₂PO₄buffer (pH 7.5) with mortar and pestle. The homogenate was then centrifuged in cold with a refrigerated centrifuge at 3000 g for 2 min and the supernatant was collected. The pellet was suspended in further 2 ml of buffer and centrifuged for 5 min at 3000 g and the supernatant was collected. The combined extract containing soluble protein was made up to 5 ml with buffer. The pellet was resuspended in 5 ml of buffer, the suspension represented insoluble protein. One ml of each of soluble and insoluble protein suspension was placed in separate centrifuge tubes and 5 ml of 5% trichloroacetic acid was added to each of them. The protein was allowed to precipitate for 30 min and then centrifuged for 5 min at 3000 g. The supernatant was discarded. The pellet was suspended in 1 ml of distilled water. Protein was determined. Proline was extracted by homogeniging fresh tissue in 0.1 M sulphosalicyclic acid. The homogenate was then centrifuged at 3000 g for 5 min and the supernatant was collected for determination of proline.

Source of Information: Bangladesh J. Bot. 42(1): 55-63, 2013 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In conclusion, if the overall effects of waterlogging on ion transport, accumulation of proteins and amino acid are coordinated, a relationship between these parameters and growth may be established. For example, waterlogging decreased soluble protein accumulation. Soluble protein may also be used as respiratory substrate. Therefore, the decrease in soluble protein content might lead to shortage of energy supply which may have negative affect on accumulation and transport of ions. These adverse effects on inorganic nutrition and biochemical process might lead to the decrease in growth.

Knowledge Management: Journal