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Effect of Gamma Radiation on the Sensitivity and Anatomy of the Larvae of Melonfly, Bactrocera Cucurbitae (coq.) (diptera: Tephritidae)

A. F. M. Aslam
Department of Zoology, Jahangirnagar University, Savar, Dhaka

A. J. Howlader
Department of Zoology, Jahangirnagar University, Savar, Dhaka

Abstract/ Executive Summary:

The melonfly, Bactrocera cucurbitae (Coq.) Diptera Tephritidae are regarded as one of the most important quarantine pest of the fruits and vegetables of Bangladesh. The fresh eggs and early instar larvae were more sensitive to radiation than the older ones. In a comparative study of the internal anatomical structures, it was found that the size of the supraoesophageal ganglion decreased remarkably when treated with approved quarantine doses of gamma radiation. The same treatment had no effect on the size of the proventriculus.

Keywords: Radiation, Bactrocera cucurbitae, Fly larvae

Location: Institute of Food and Radiation Biology, AERE, Savar.

Start Date: 01-01-1995

End Date: 31-12-1995

Program Area: Pest Management

Commodity: Melon

Objectives:

To investigate the effect of gamma radiation in the reduction of the total surface area of some target organs such as proventriculus and supraoesophageal ganglion of the fly larvae

Methodology:

The adults of B. cucurbitae were reared in the laboratory in aluminium framed and nylon covered cages (30cm x 20cm x 20cm) at an ambient temperature (28±°C) with 80±1% rh and a 12h dark cycle. The initial fly stock was raised from a collection of infested wild fruits and vegetables. The adults were supplied with a 3:1 yeast-sugar solution and water with soaked cotton balls. The eggs were collected by placing bitter gourds in the cage for 2hrs every time. Larval rearing was maintained in the infested whole bitter gourds and supplemented with an artificial diet composed of wheat bran, sugar, brewer's yeast, citric acid, sorbic acid and water. At this temperature regime and diet it took about 28-34 hrs for egg hatch and 10-14 days for the larvae to reach pupal stage. The radiation sensitivity of the fresh and older eggs and the different larval stages were observed by exposing them to different doses of gamma radiation. The 24 hrs old eggs along with the bitter gourds were exposed to 0, 50, 100, 150 and 200 Gy of gamma radiation for the study of gamma irradiation effect on the target organs. Irradiation treatment were given from a Co⁶⁰source (strength 1900 Ci, December, 1995) installed at the Institute of Food and Radiation Biology, AERE, Savar. When the emerging larvae attained their 3rd instar stage, they were collected, dissected and studied under a binocular microscope in a cavity slide with saline solution (pH 6.8). A minimum of 20 larvae at each dose level were dissected. Measurements were taken with the help of oculometer syncronized with stage micrometer.

Source of Information: Bangladesh Journal of Life Science, June 1997, Vol.9(2), pp:37-46, ISSN:1023-0858.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In a comparative study of the internal anatomical structures, it was found that the size of the supraoesophageal ganglion decreased remarkably when treated with approved quarantine doses of gamma radiation. The same treatment had no effect on the size of the proventriculus. Our findings indicate that the reduction of supraoesophageal ganglion in the radiation treated larvae may be a direct method for identifying treated larvae from the non-treated one. It may also be suggested that gamma radiation may be used as an alternative to the EDB for the quarantine purpose of the melonfly, Bactrocera cucurbitae (Coq.).

Knowledge Management: Journal