Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Evaluation of Fungicidal Responses on the Growth of Pathogenic Rhizoctonia Solani Kuhn, Antagonistic Binucleate Rhizoctonia and Trichoderma Harzianum Rifai

Md. Maniruzzaman Khandaker
Department of Botany, Government Saadat College, Tangail, Bangladesh

MD. KHURSHED ALAM BHUIYAN
Department of Plant Pathology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh.

ABUL KHAIR
Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh.

Abstract/ Executive Summary:

Two pathogenic isolates of Rhizoctonia solani Kuhn causing stem canker/black scurf disease of potato plants and four antagonist isolates, two of binucleate Rhizoctonia and two of Trichoderma harzianum Rifai were isolated from crop fields and evaluated in vitro for their fungicidal responses against eight fungicides. Vitavax was effective in inhibiting the growth of R. solani and binucleate Rhizoctonia but it did not inhibit the growth of T. harzianum at 100 ppm concentration. Terraclor Super X, Dithane M 45 and Boric acid are the fungicides which at 100 ppm concentration did not inhibit the growth of antagonist isolates of T. harzianum and binucleate Rhizoctonia but inhibited the growth of isolates of R. solani to some extent. The in vitro findings suggest that any one of these three fungicides along with antagonist isolates of binucleate Rhizoctonia and T. harzianum can be used as biocontrol agents to reduce soil borne inocula of R. solani.

Keywords: Rhizoctonia solani; Binucleate; Trichoderma harzianum; Fungicide

Location:

Start Date:

End Date:

Program Area: Pest Management

Commodity: Fungicide

Objectives:

To achieve the above objective the present work was aimed to find out a fungicide/s following in vitro evaluation which will have at least some inhibiting effect on the growth of the plant pathogen R. solani but having no such effects on its antagonist isolates of binucleate Rhizoctonia and T. harzianum.

Methodology:

Four concentrations, viz. 0, 1, 10 and 100 ppm in term of active ingredient were used for all fungicides. Requisite amount of fungicide was mixed with PDA medium. Autoclaved medium was poured into 90 mm diameter Petri dishes @ 20 ml per plate. After solidification of the medium, the plates were inoculated with 5 mm diameter mycelial blocks cut from 3-day-old colony of R. solani isolates (BTB115 and DK64). Mycelial blocks from cultures of binucleate Rhizoctonia (GK206 and CD169) and T. harzianum (CU1 and W9) isolates were also transferred to fungicides amended PDA plates. There were four replications for each treatment. The plates were incubated at 25 + 1ºC for ten days. Data on radial mycelial growth of the colonies was recorded after five days of inoculation. Per cent inhibition of growth was calculated with the following formula (Sundar et al. 1995): % inhibition of growth = (X-Y)/X by X, where, X = growth of pathogen/antagonist without fungicide and Y = mycelial growth of the pathogen with fungicide in the medium.

Source of Information: Bangladesh J. Bot. 39(1): 107-110, 2010 (June)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The in vitro findings suggest that any one of these three fungicides along with antagonist isolates of binucleate Rhizoctonia and T. harzianum can be used as biocontrol agents to reduce soil borne inocula of R. solani.

Knowledge Management: Journal