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Effects of Nacl Stress on Accumulation of K+, Na+, Cl-, No3 -,sugar and Proline Contents in the Seedlings of Triticale-i

RIFAT SAMAD
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

J L K ARMOKER
Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh.

Abstract/ Executive Summary:

NaCl stress at 50 - 150 mM NaCl caused a 12 - 16-fold increase in Na⁺ accumulation in the root and 3 - 10-fold in the shoot of Triticale-I with a concomitant decrease in that of K⁺ and Cl^- . NaCl stress increased the accumulation of reducing, total sugars and proline both in the root and shoot of the seedlings. The mechanism of salt tolerance of Triticale-I plants with respect to ionic relation and accumulation of sugars and proline were discussed.

Keywords: Ion transport, Sugar, Proline,Triticale

Location: Dhaka University

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Triticale

Objectives:

To determine the effect of NaCl- salinity stress on the accumulation and distribution of K + , Na + , Cl - , NO₃ - and reducing and total sugars and proline.

Methodology:

Triticale ( Triticosecale Wittmack), a hybrid between wheat and rye is tolerant to environmental stress and offers an important potential protein source for both human consumption and animal feed. Triticale seeds were collected through the courtesy of Bangladesh Agriculture Research Institute (BARI), Gazipur. Plants were grown in water culture to study the accumulation of ions (Na ⁺ , K ⁺ , Cl ^- and NO₃ ^- ), reducing and total sugar and proline. Seeds were surface sterilized according to Samad and Karmoker (2012). Then the seeds were spread over a cotton gauge placed in a lid having nine holes (3 cm dia.) and the lid with seeds was placed on a bucket containing 5 liter of modified half-strength Hoagland solution (Hoagland and Arnon 1950). The bucket was painted black to avoid the exposure of light to the roots. The buckets were kept in dark for 48 h to facilitate the germination of seeds. After germination, the buckets with the seedlings were placed in a light bank at a day/night temperature of 25ºC ± 1ºC/18ºC ± 1ºC and day/night length of 14 h/10 h and light intensity was 160 μE/m ² /sec. Relative humidity was 75% by day and 85% at night. Half-strength Hoagland solution was used as control. The salinity treatments were made by using 50, 100 and 150 mM NaCl solutions in half-strength Hoagland solution. Solutions of control and treatments were aerated continuously by an air compressor. After 3 days, seedlings were plugged to each hole of the lid with spongy foam for rendering support. Nutrient solution was completely replaced every 7 days. Distribution of ions was measured in roots and shoots of the seedlings after 7, 14 and 21 days of salinity tr eatments. Three replicates were used in each treatment. The tissues were dried in an oven at 80^o C for 72 h to attain constant weight. Na + , K ⁺ and Cl^- were extracted from dry tissue by boiling in water bath with two changes of 10 ml distilled water contained in test tubes. Na + , K ⁺ and Cl ^- and NO₃^- contents were measured according to Begum (1993) and Cataldo et al. (1975) respectively. Proline was measured following Bates et al. (1973). Reducing and total sugars were extracted by boiling fresh root and shoot tissue in two changes of 5 ml of 80% ethanol for five minutes in a hot water bath according to Karmoker and Van Steveninck (1979) and measured following Somogyi-Nelson method (Nelson 1944, Somogyi 1952) and Dubois et al. (1956)

Source of Information: Bangladesh J. Bot. 42(2): 189-194, 2013 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

NaCl stress (50 - 150 mM) caused a 12- to 16-fold increase in the accumulation of Na ⁺in the root of Triticale seedlings at 7-day of treatment. K ⁺ accumulation in the root and the shoot was decreased by 93, 85 and 71% at 50, 100 and 150 mM NaCl treatment respectively at 7-day of treatment. NaCl stress (50 - 150 mM) caused a 66 to 88% decrease in accumulation of Cl ^- in the root of Triticali seedlings and a 14-76% decrease in Cl ^- accumulation in the shoot at 7-day of treatment. In the root, NO₃^- accumulation was decreased by 37 - 51% from 50 - 150 mM NaCl and the decrease was 35 - 65% in the shoot at 7-day of treatment. At 7-day of treatment, the accumulation of reducing sugar increased gradually from 1.2- to 2.8-fold in the root and 1.4- to 6.8-fold in the shoot when the seedlings were subjected to 50-150.mM NaCl salinity. Salinity-induced dramatic stimulation of accumulation of sugars and proline inTriticale-I helps maintain the osmotic potential of cytoplasm of cells which is important forsurvival of plants under stress.

Knowledge Management: Journal