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Development of Doubled Haploid Through Anther Culture

Dr.Md Enamul Hoque
CSO and Head (In Charge)
Biotechnology Division,BRRI-Gazipur

Dr.Md.Shamsher Ali
CSO
Biotechnology Division, BRRI-Gazipur

S M Hisam Al Rabbi
SO
Biotechnology Division, BRRI-Gazipur

Dr.Nilufar Yasmin Shaikh
SSO
Biotechnology Division,BRRI-Gazipur

Ripon Kumar Roy
SO
Biotechnology Division, BRRI-Gazipur

Abstract/ Executive Summary:

The study was conducted with the objective to develop double haploid line with high yield and short duration. For this study five F₁ populations were grown in the net house under optimum management. Boots with appropriate stage (uninucliate/mid uninucleate at reduction division stages) were collected from F₁ plants. The boots were pre-incubated in a refrigerator at 8ºC for 8-10 days. Anther culturing combined with conventional breeding has proved to be an effective tool for developing varieties with the desired traits in short time. Therefore, anthers of promising F₁s are cultured for generating potential high yielding and early maturity rice lines.

Keywords: Anther, Callus induction, Haploid lines

Location: Biotechnology Division, BRRi-Gazipur

Start Date: 01-07-2008

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To develop double haploid line with high yield and short duration

Methodology:

For this study five F₁ populations were grown in the net house under optimum management. Boots with appropriate stage (uninucliate/mid uninucleate at reduction division stages) were collected from F₁ plants. The boots were pre-incubated in a refrigerator at 8ºC for 8-10 days. The panicles were opened from the boots and cut into small pieces. They were then surface sterilized by immersing in 70% ethanol for 2-3 minutes and then placed onto the sterile filterpaper in Petri dishes to remove access alcohol. For callus induction, anthers were incubated in two different media (KA and KE) and kept in an incubator under dark condition at 25ºC until callus initiation and subsequent growth up to appropriate size (2.0 mm). Calli were then transferred into regeneration medium (MS medium containing 1.0 mg/l NAA+ 1.0 mg/l Kn) and cultures were incubated in a cultured room under cool white Fluorescent lamps (approximately 1000 lux, at 25ºC) for regeneration. Eight weeks after transfer of calli into the regeneration medium, data were taken on number of anther plated, number of callus produced, number of green and albino plant regenerated.

Source of Information: Proceedings of the BRRI Annual Internal Review for 2008-09

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Highest number of calli was obtained from hybrid anthers of BR802-78-2- 1-1-1×BR6157-R1-1 in KA medium ). Calli obtained from all the five F₁ populations wereincubated in regeneration medium but no green plant regenerated.

Knowledge Management: Report/Proceedings