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Field Investigation on the Efficacy of Salmonella Vaccine Prepared at Bangladesh Agricultural University

M. A. S. Bag
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. M. Amin
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. B. Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Y. A. Arafat
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

M. Salim
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

I. H. Rasel
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

U. H. Majumder
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The research work was performed to investigate the immunogenicity of Salmonella vaccine produced at LPVRPC, BAU Mymensingh. The vaccination was performed at the Phenix Hatchery Ltd. Gazipur in Hy-sex brown and HY-sex white chicken designated as group A and group B respectively. Group A and B were subdivided into A1, A2, A3, B1, B2 and B3 groups containing eight birds each. Group C was maintained as unvaccinated control. Birds were immunized following schedule of the LPVRPC. Each bird was vaccinated SC at six weeks of age followed by a subsequent booster dose after 45 days. After four weeks of primary vaccination the mean PHA antibody titres were 96.00±34.21 in group A1 and 96.00±34.21 in B1 group. Prebooster vaccination the mean PHA antibody titres were 88.00±33.12 in group A2 and 88.00±33.12 in B2 group. At four weeks of booster vaccination the mean PHA antibody titres were 104.00±33.12 in group A3 and 104.00±33.12 in B3 group. The mean ± PHA antibody titre in chickens of group C was ≤ 4.0±0.00. Salmonella vaccine prepared at (LPVRPC) Department of Microbiology and Hygiene, BAU induces satisfactory level of antibody in chickens determined by PHA test conducted in an on-farm study of layer chickens.

Keywords: Immunogenicity, Salmonella, Vaccine, Antibody, PHA titre

Location: BAU, Mymensingh

Start Date: 00-07-2010

End Date: 00-12-2010

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

(i) To Isolate and identify Salmonella spp from infected chicken reared in farm

(ii)To investigate of immunogenicity of Salmonella vaccine produced by LPVRPC

Methodology:

The present research was conducted during the period of July 2010 to December 2010. Salmonella vaccine as prepared by LPVRPC at BAU was investigated immunogenicity for measured in term of production of antibody in vaccinated chicken determined by PHA test. Five weeks aged Hy-sex chicken (white and brown) were selected for these experiment. These chicken were divided into two groups- (Vaccinated group and Unvaccinated group) under this vaccinated group collection of blood prior to vaccination from Hy-sex brown A and Hy-sex white B. Primary vaccination were done at 6 weeks of age in Hy-sex brown A and Hy-sex white B. Post primary vaccination bleeding occurred at 10 weeks of age or 30 days after primary vaccination and Prebooster bleeding at 11 weeks of age or 37 days after primary vaccination. Booster vaccination of Hy-sex brown A and Hy-sex white B were done at 12 weeks of age. Post booster bleeding at 16 weeks of age (A3 and B 3 ) or 74 days after primary vaccination. Then collection of sera and PHA test was performed. The sensitivity of PHA test depends upon the use of soluble antigens. Five milliliters of 2.5% HRBC and 5 ml of 1:20000 dilution of tannic acid was taken in a test tube and mixed thoroughly. The cells were centrifuged at 2000 rpm for 10 minutes; the sediment was then washed with PBS. An amount of 50 µl of PBS was first poured in each well up to 8th well of horizontal row of microtitre plate, 50 µl of test serum was added in the 1st well. Two fold dilutions of serum ranging from 1:2 to 1:256 were made by transferring 50µl of the mixture from the 1st well to 2nd well and thus continuing successively up to the 8th well from where an excess amount of 50 µl of the mixture was poured off. A volume of 50 µl 0.5% somatic antigen sensitized hRBC was taken in each of the eight wells. The content of the wells of the test system and control were mixed by gentle agitation of the microtitre plate and kept at room temperature for 4 to 5 hours. The PHA titre was the highest dilution of test sera were complete haemagglutination occur due to the reaction of specific antibody and antigen sensitized tanned HRBC. The results were recorded by deposition of a diffuse thin layer of clumping of RBC on the bottom of the wells, which indicated HA positive, and a compact buttoning with clear zone indicated HA negative. The reciprocal of the highest dilution of sensitized tanned HRBC was considered as titre of the serum.

Source of Information: Bangl. J. Vet. Med. (2015). 13 (1): 5-9 ISSN: 1729-7893 (Print), 2308-0922 (Online)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The suspected disease of chicken at Phenix Hatchery Ltd was caused by Salmonella spp. Salmonella vaccine prepared at (LPVRDC) Department of Microbiology and Hygiene, BAU, Mymensingh induces good level of antibody in chickens determine by PHA test conducted in an on farm study of layer chickens.

Knowledge Management: Journal