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In Vitro Conservation of Taro (colocasia Esculenta Var. Globulifera) as Influenced by Mannitol

M. K. R. BHUIYAN
Principal Scientific Officer
TCRC, Bangladesh Agricultural Research Institute (BARI), Gazipur.

M. J. HOSSAIN
Director
TCRC, BARI , Gazipur

M. M. HAQUE
Senior Scientific Officer
Farm Division, BARI, Gazipur, Bangladesh.

Abstract/ Executive Summary:

In vitro conservation of germplasm plays a vital role in maintenance breeding and also has many advantages over the conventional system. The experimental results for conservation of Colocasia sp. also proved this. In relation to explants and osmoticum, meristem and axillary bud could be conserved for 24 months while meristem-base died after 6 months. Mannitol as osmoticum @ 4% performed nicely to conserve Colocasia upto 24 months. Only meristem and axillary bud could be conserved for 24 months with the use of 4 % mannitol. But other level of mannitol remained culture alive for varying periods (6 to 12 months). After 24 months, the plant height was 6.5 cm for the meristem and 6.4 for axillary bud.

Keywords: Colocasia; Mannitol; Meristem and Axillary bud

Location: Tuber Crops Research Centre (TCRC), BARI, Joydebpur, Gazipur.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Aroids

Objectives:

To determine in vitro conservation of taro as influenced by mannitol.

Methodology:

For conservation of taro an experiment was conducted at tissue culture laboratory of the Tuber Crops Research Centre (TCRC), BARI, Joydebpur, Gazipur. Well sprouted cormels of mukhikachu var. ‘Bilashi’ ( Colocasia esculenta var. globulifera ) was used. The axillary bud ( ca . 0.2 - 0.4 mm) and meristem ( ca . 0.5 mm) were excised from sterilized sprout of cormel and used as explant. After excising the meristem remaining meristem base was also used as explant. The in vitro growing medium contained mineral salts and vitamins plus mannitol according to the treatment. Three types of explants (axillary bud (Ab), meristem (M) and meristem base (Mb) and four levels of mannitol (0, 2, 4 and 6 %) were used in this experiment. Cultures were maintained under light (16 h photoperiod). Complete Randomized Design (CRD) was followed having five replications each having 5 tubes. The cultured tubes were stored for 24 months. The total number of plants survived at different intervals after in vitro multiplication was recorded. The percent plant survival at 2, 4, 6, 8, 12 and 24 months after in vitro multiplication was calculated by the formula as follows: % plant survival = (Total number of tubes with survived plant/Total number of tubes cultured) X 100. Plant height at different intervals was measured by destroying the test tube. Data were analyzed statistically and mean separation was done at 1% level of probability by DMRT.

Source of Information: Bangladesh J. Agril. Res. 41(1): 67-74, March 2016, ISSN 0258-7122

Article Link (Online Journal): DOI: http://dx.doi.org/10.3329/bjar.v41i1.27668

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Only meristem and axillary bud could be conserved for 24 months with the use of 4 % mannitol. But other level of mannitol remained culture alive for varying periods (6 to 12 months). After 24 months, the plant height was 6.5 cm for the meristem and 6.4 for axillary bud.

Knowledge Management: Journal