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Micro-cloning in Commercially Important Six Bamboo Species for Mass Propagation and at a Large Scale Cultivation

S. A. M. Nurul Islam
Bangladesh Forest Research Institute, Sholoshahar, P.O. Amin Jute Mills, Chittagong-4000, Bangladesh.

Md. Mahbubur Rahman
Bangladesh Forest Research Institute, Sholoshahar, P.O. Amin Jute Mills, Chittagong-4000, Bangladesh.

Abstract/ Executive Summary:

In vitro cloning of some commercially important bamboos (viz. Bambusa balcooa, B. nutans, B. salarkhanii, B. vulgaris, B. vulgaris var. striata and Thyrsostachys oliveri) were tried by using nodal buds. Excised shoots were used as explants in transfer cultures and BAP (1 - 5 mg/l) was found to be effective growth regulator in liquid media for inducing multiple shoots (3 - 30). Half strength MS was supplemented with NAA (1 - 3 mg/l) and IBA (1 - 5 mg/l) for root induction. Two to three weeks old 3 - 5 cm long shoots in cultures were suitable for inducing rooting. Frequent changes of rooting media had improved production of rooted plant lets. Rooted plant lets were cultured in non-sterile water in the growth room for one week and after the plantlets were transferred to the propagation bed. The plant lets in propagation beds produced mini-clumps with 3 - 10 shoots within five - eight weeks. Rooted shoots of the mini-clumps were separated into two - five parts. The younger parts of mini-clumps were transplanted in the propagation bed for further proliferation of shoots; older parts were transferred in the soil-filled bags for field planting. These bagged plant lets were vigorous in growth and found suitable for large scale cultivation.

Keywords: Nodal bud, Cloning, Mini-clump, Proliferation, Cultivation

Location: Bangladesh Forest Research Institute, Sholoshahar, P.O. Amin Jute Mills, Chittagong-4000, Bangladesh.

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Bamboo

Objectives:

To determine the Micro-cloning in Commercially Important Six Bamboo Species

Methodology:

Buds with 1.0 - 1.5 cm nodal segments were used to initiate and establish in vitro culture. Nodal buds were collected from branches of one - two years old culms of six different bamboos (viz. Bambusa balcooa, B. nutans, B. salarkhanii, B. vulgaris, B. vulgaris var. striata and Thyrsostachys oliveri). Clumps of donor bamboos were about 10 - 40 years old. Explants of nodal buds were collected in late February or in March. Nodal buds were green and generally buds of upper nodes were initiated to sprout. Collected buds were cleaned, sized and washed under running water. HgCl2 (0.1%) and a few drops Tween 20 (Polyxyethylene sorbitan Monolaurate) were used to surface sterilized explants. The sterilized nodal buds were cultured in semi-solid gel of MS and supplemented with BAP (1.0 mg/l). Selected buds sprouted within three - four weeks. Prior to final transfer into soil, plant lets which were already in soil with well developed roots were cultured for one - two weeks in water. Within a period of three - four months the plantlets transferred to the propagation bed produced mini-clumps of shoots in abundance. Roots grew on the shoot bases of miniclumps. The rooted shoots of mini-clumps proliferated further when individual mini-clumps were separated and planted in the specially prepared planting beds. This procedure was found most suitable for large scale shoots multiplication or for their storage in bags until field planting. Bagged plantlets were planted in June, 2004 on different locations at the bambusetum in BFRI farmer’s field at Harbang, Jahangirnagar University campus at Savar near Dhaka city and Bangladesh Agriculture Research Institute (BARI) research station at Chandraghona, Chittagong. Data on the field performance i.e., the growth of six to nine months old bamboo species and their survival rate were collected in April, 2005.

Source of Information: Plant Tissue Cult. & Biotech. 15(2): 103-111, 2005 (December)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Frequent changes of rooting media had improved production of rooted plant lets. Rooted plant lets were cultured in non-sterile water in the growth room for one week and after the plantlets were transferred to the propagation bed. The plant lets in propagation beds produced mini-clumps with 3 - 10 shoots within five - eight weeks. Rooted shoots of the mini-clumps were separated into two - five parts. The younger parts of mini-clumps were transplanted in the propagation bed for further proliferation of shoots; older parts were transferred in the soil-filled bags for field planting. These bagged plant lets were vigorous in growth and found suitable for large scale cultivation. Here, we have shown that use of nodal buds is an alternative reproducible and dependable method for cloning of all the six bamboo species that we have tried. We have also shown that proliferation of mini-clumps of micro propagated plant lets is one more powerful and reliable method to attain the same objective, namely large scale multiplication of bamboo species available in the country.

Knowledge Management: Journal