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Development of Doubled Haploid Lines Through Anther Culture(2009-10)

Dr. Md .Shamsher Ali
CSO
Biotechnology division, BRRI-Gazipur

Ripon Kumar Roy
SO
Biotechnology division, BRRI-Gazipur

S,M.Hisam Al Rabbi
SO
Biotechnology division, BRRI-Gazipur

Dr,Enamul Hoque
CSO and Head (In Charge )
Biotechnology division, BRRI-Gazipur

Abstract/ Executive Summary:

Objective of the study was to develop double haploid line with high yield, earliness, fine grain and aroma through anther culture For this study twelve F1s populations were grown in the net house under optimum management. Boots with appropriate stage (uninucliate/mid uninucleate at reduction division stages) were collected from F1 plants. Anther culture combined with conventional breeding has been proved to be an effective tool for developing varieties with the desired traits in a short period of time. Therefore, anthers of promising F1s are cultured for generating potential high yielding and early maturity rice lines.

Keywords: Anther, callus induction, Haploid lines

Location: Biotechnology Division, BRRI-Gazipur

Start Date: 01-01-2009

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To develop double haploid line with high yield, earliness, fine grain and aroma through anther culture

Methodology:

For this study twelve F1s populations were grown in the net house under optimum management. Boots with appropriate stage (uninucliate/mid uninucleate at reduction division stages) were collected from F1 plants. The boots were preincubated in a refrigerator at 8ºC for 8-10 days. The panicles were opened from the boots and cut into small pieces. They were then surface sterilized by immersing in 70% ethanol for 2-3 minutes and then placed onto the sterile filter paper in Petri dishes to remove excess alcohol. For callus induction, anthers were incubated into two different media (KA and KE) and kept in an incubator under dark condition at 25ºC until callus initiation and subsequent growth up to appropriate size (appx. 2.0 mm diameter). Calli were then transferred into regeneration medium (MS medium containing 1.0 mg/l NAA+ 1.0 mg/l Kn). Cultures were incubated in a culture room under cool white Fluorescent lamps (approximately 1000 lux, under 25ºC) for regeneration. Eight weeks after transfer of calli into the regeneration medium, data were taken on number of anther plated, number of callus produced, number of green and albino plant regenerated.

Source of Information: Proceedings of the BRRI Annual Internal Review for 2009-10

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Highest number of calli were obtained from hybrid anthers of Arman sarder × BR10 in KE medium. Calli obtained from all the four F₁ populations were incubated in regeneration medium and only 19 green plants regenerated .

Knowledge Management: Report/Proceedings