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In Vitro Studies on the Fungicidal Effect on Trichoderma Species in Tea Plantation

M. S. ISLAM
Division of Plant Pathology, Bangladesh Tea Research Institute (BTRI), Srimangal- 3210

M. ALI
Division of Plant Pathology, Bangladesh Tea Research Institute (BTRI), Srimangal- 3210

M. S. RAHMAN
Assistant Manager
Haychem (Bangladesh) Limited, Dhaka, Bangladesh

Abstract/ Executive Summary:

Tolerance of Trichoderma species collected from tea plantation to fungicides was evaluated in vitro using poisoned food method. Fungicides like Carbendazim 50 WP, Copperoxychloride 50 WP, Hexaconazole 5 EC, and Propiconazole 25 EC were used at two lower and two upper of recommended doses. The growth of T. harzianum was mostly inhibited by the Carbendazim 50 WP and Propiconazole 25 EC with the recommended doses; while T. viride could grow easily with the said doses. Both species of Trichoderma grew easily in medium containing Copperoxychloride even at highest doses. Hexaconazole 5 EC proved to be highly toxic with no growth of Trichoderma harzianum in treatments containing 40.0 and 42.05 ppm. At highest concentration (42.5 ppm) of Hexaconazole 5 EC, T. viride grew after 72 hours of incubation. It was 14.44% over the control on 4^th day of plating.

Keywords: In vitro, Fungicidal effect, Trichoderma species, Tea

Location: Bangladesh Tea Research Institute, Srimangal

Start Date: 00-00-2009

End Date:

Program Area: Pest Management

Commodity: Tea

Objectives:

To screen Trichoderma species viz., T. harzianum and T. viride for tolerance to fungicides.

Methodology:

The experiment was carried out at the Plant Pathology laboratory of Bangladesh Tea Research Institute, Srimangal during 2009. Potato Dextrose Agar (PDA) medium was prepared in the laboratory. Medium and necessary glassware were sterilized in autoclave (Islam et al., 2008). Soils were collected from 15 different marks of tea areas under main farm of Bangladesh Tea Research Institute at 0-9 inches. All collected samples were mixed thoroughly to make a composite sample. One gram (dry weight basis) soil was mixed into 9 ml of sterile distilled water. Then 1 ml of suspension was taken into another tube containing 9 ml of sterile distilled water to make 1:10 solution. This serial dilution technique was continued up to 1: 10,000. From the final dilution (1: 10,000), 1 ml suspension was transferred to each of the three petridishes. 20 ml of melted agar medium was poured in each plate and mixed with the suspension by giving a gentle whirling motion to the plate and allowed them to incubate in room temperature (Islam et al., 2008). Trichoderma harzianum and T. viride were identified by using CMI sheet. Sub culturing was performed and the culture of Trichoderma in pure form was maintained. Tolerance to fungicides of Trichoderma species was evaluated using poisoned food method (Nene and Thapliyal, 1982). Fungicides viz. Carbendazim 50 WP (325, 350, 375, 400 and 450 ppm), Copperoxychloride 50 WP (750, 1000, 1250, 1500 and 1750 ppm), Hexaconazole 5 EC (32.5, 35.0, 37.5, 40.0 and 42.5 ppm) and Propiconazole 25 EC (162.5, 175.0, 187.5, 200.0 and 212.5 ppm) were added to PDA medium. The concentrations of fungicides were selected as two lower and two upper doses of recommended dose (Huq et al., 2007). PDA medium without fungicides served as control. A 5mm inoculum disc of Trichoderma species was cut from the margin of actively growing colony and placed in centre of each Petri plate. Petri plates were incubated at room temperature. Five Petri plates were used for each treatment. The experiment was laid out in a Complete Randomized Design with four replications. Radial growth of Trichoderma species was observed daily. Data subject to analysis of variance by MSTAT computer program. Data were transformed to minimize the dependence of mean over variance. Mean separation was done by Duncun’s Multiple Range Test (Kader et al., 2000).

Source of Information: Bangladesh J. Agril. Res. 36(4): 677-683, December 2011, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The growth of T. harzianum was mostly inhibited by the Carbendazim 50 WP and Propiconazole 25 EC with the recommended doses; while T. viride could grow easily with the said doses. Both species of Trichoderma grew easily in medium containing Copperoxychloride even at highest doses. Hexaconazole 5 EC proved to be highly toxic with no growth of Trichoderma harzianum in treatments containing 40.0 and 42.05 ppm. At highest concentration (42.5 ppm) of Hexaconazole 5 EC, T. viride grew after 72 hours of incubation. It was 14.44% over the control on 4th day of plating.

Knowledge Management: Journal