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Effect of Preserved Seeds Using Different Botanicals on Seed Quality of Lentil

A. KHATUN
Principal Scientific Officer
Irrigation and Water Management Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur-1701

G. KABIR
Professor
Department of Botany, Rajshahi University, Rajshahi 6205

M. A. H. BHUIYAN
Senior Scientific Officer
Soil Science Division, BARI, Joydebpur, Gazipur-1701

D. KHANAM4
Senior Scientific Officer
Biotechnology Division, BARI, Joydebpur, Gazipur-1701, Bangladesh.

Abstract/ Executive Summary:

Laboratory studies were conducted with leaf powder of three plants to show the preservative effect for maintaining the quality of lentil seeds in storage. After processing and drying, seeds were preserved with different botanicals and stored them in earthen pots for eight months. Botanicals, such as whole leaf powder of neem (Azadirachta indica), dholkalmi (Ipmoea sepiara), and bishkatali (Polygonum hydropiper) were used at a dose of 5% w/w (25 g botanical per 500 g of lentil seeds). The lentil seeds were stored till next planting time and seed quality, such as moisture content, germination capacity, root length, shoot length of the seedlings and vigour index were observed. The highest values for all these characters except moisture content were significant when the seeds were preserved with neem leaf powder and bishkatali. Among three botanicals, dholkalmi was less effective.

Keywords: Lentil, botanicals, storage. seed quality

Location: Bangladesh Agricultural Research Institute (BARI), Jovdebpur, Gazipur.

Start Date: 00-00-2003

End Date: 00-00-2004

Program Area: Seed Technology

Commodity: Lentil

Objectives:

To determine the effects of three botanicals viz., leaf powder of neem (Azadirachia indica), dholkalmi (Ipmoea sepiara), and bishkatali (Polygonum hydropiper) on the seed quality of lentil.

Methodology:

Studies were carried out in the laboratory of Seed Technology Division, Bangladesh Agricultural Research Institute (BARI), Jovdebpur, Gazipur. Lentil (BARI Masur-2) seeds used in the study were collected from Pulses Research Centre’s experimental field, BARI, Gazipur during the rabi seasons of 2003-04 kept in earthen pots and stored in normal condition. The test botanicals were leaf powder of neern (Azadirachta indica), dholkalmi (Ipmoea sepiara) and bishkatali (Polygonum hydropiper). The leaves of these plants were collected from different villages of Gazipur. The collected leaves were dried under ambient room temperature (27°C to 34°C), grinded separately by a hand grinder and passed through a 60- mesh sieve to get fine powder. Clean and fresh lentil (BARI Masur-2) seeds were taken from the seed lot and sun dried to maintain 9-l0% moisture content. The seeds were then divided into four parts. One part was considered as control (T₄) i.e. without any botanical treatment. Other three parts were treated with three different botanicals at the dose of 5% w/w i.e., 25 g/500 g of lentil seeds (Bhuiyah, 2001) with six replications. Before storage, seeds were analyzed for germination, moisture content, and vigour index. The seeds were stored in March till next planting time and seed quality was observed during August to September in 2004 and 2005. Data on moisture content, germination capacity, root length, shoot length, root plus shoot length and vigour were recorded. Average germination percentage of the seed lot before storage was 90% and average moisture content of the seed before storage was 9%. The moisture content of seed samples were determined following the rule of ISTA, 1976. Germination test was carried out according to ISTA rule of 1976. For each treatment, 100 seeds were placed in Petri dishes (8.50 cm diameter). Six replicates were used. The Petri dishes were kept on a laboratory table at room temperature (25 ± 2°C). After ten days, normal, abnormal, and diseased seeds were counted. After eight days, ten plants were randomly selected from each replicate of each treatment. The seedlings were cut into root and shoot parts. They were measured (in cm) and the mean value was computed. Seed vigour was calculated as percent germination x length of seedling (Reddy and Khan, 2001). The data for different parameters were compiled and subjected to statistical analysis following a computer IRRISTAT and MSTAT package programme (Freed, 1992) adjusting the means. The correlation co-efficient and regression analysis were done for different variables wherever needed using Microsoft Excel Programme 1997.

Source of Information: Bangladesh J. Agril. Res. 36(3) : 381-387, September 2011, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest values for all these characters except moisture content were significant when the seeds were preserved with neem leaf powder and bishkatali. Among three botanicals, dholkalmi was less effective.

Knowledge Management: Journal