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In Vitro Regeneration of Brinjal (solanum Melongena L.)

B. P. RAY
Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.

L. HASSAN
Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.

K. M. NASIRUDDIN
Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh.

Abstract/ Executive Summary:

The effect of different explants and concentrations of BAP and NAA on induction of callus and plant regeneration of brinjal cv. Jhumki were investigated. The treatment combinations were BAP (0. 2.0. 3.0, and 4.0 mg/l) and NAA (0. 0.1, 0.5, and 1.0 mg/l). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem, and 8.2 days required for callus induction. The highest fresh weight of callus was 1.12g from stem and 0.48g from root. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. All regenerated plantlets survived in normal environment.

Keywords: NAA, BAP, Regeneration, Brinjal.

Location: Bangladesh Agricultural University (BAU), Mymensingh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Brinjal

Objectives:

To investigate the effect of different explants and concentrations of BAP and NAA on induction of callus and plant regeneration of brinjal cv. Jhumki .

Methodology:

Healthy seeds of brinjal cv. Jhumki were collected from BARI. The seeds were then washed thoroughly in running tap water. Special care was taken to avoid all types of injury. The surface sterilization of these seeds were carried out under a Laminar Air Flow Cabinet. The floated seeds were discarded and others were rinsed in 70% ethyl alcohol for one minute, and then thoroughly washed with sterilized distilled water. The alcohol treated seeds were immersed into 0.1% HgCl₂ solution for 8-10 minutes, few drops Tween-20 per 100 ml was also added at that time. The seeds were then washed 5-6 times with sterilized distilled water. The seeds were then ready for placement into the media. Sterilized seeds were placed into seed germination medium in Petri dishes. Six seeds were placed in each Petri dish. The culture was then incubated in dark till the germination of seeds. These were then transferred to 16 hours light for normal seedling growth, MS basal medium with different concentrations and combinations of BAP (0. 2.0, 3.0, and 4.0 mg/l) and NAA (0, 0.1, 0.5, and 1.0 mg/l) were used. Six pieces (2-3mm each piece) of stem segments were arranged horizontally on each Petri dish and gently pressed into the surface of the sterilized culture medium. The Petri dish was covered and sealed with Para film. Root tip segments (0.5mm) were placed on a sterilized Petri dish under a Laminar Air Flow Cabinet. The Petri dish was covered and sealed with Para film. Data on Days of callus initiation; Percent callus: Nature of callus; Abundance of callus; Weight of callus; Days to shoot initiation; Days to root induction ;Days to root induction; Shoot length; Number of callus with shoot (per cent of shoot or plant regeneration); Number of shoots per callus (Average number of shoots per callus); Total number of shoots per petridish; Number of shoots with root and Number of regenerated plantlets were recorded. The data for the characters of the present study were statistically analyzed wherever applicable. The experiments were arranged in Completely Randomized Design (CRD). The analysis of variance for different characters was performed and means were compared by the Duncan’s Multiple Range Test (DMRT).

Source of Information: Bangladesh J. Agril. Res. 36(3) : 397-406, September 2011, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem, and 8.2 days required for callus induction. The highest fresh weight of callus was 1.12g from stem and 0.48g from root. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. All regenerated plantlets survived in normal environment.

Knowledge Management: Journal