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Effect of Relative Humidity, Initial Seed Moisture Content and Storage Container on Soybean (glycine Max L. Meril.) Seed Quality

M. R. ALI
Senior Scientific Officer
RARS, Bangladesh Agricultural Research Institute (BARI), Jamalpur

M. M. RAHMAN
Professor
Department of Agronomy, Bangladesh Agricultural University (BAU), Mymensingh

K. U. AHAMMAD
Senior Scientific Officer
RARS, BARI, Jessore, Bangladesh.

Abstract/ Executive Summary:

To find out the effect of storage relative humidity, seed moisture content and type of storage container on soybean seed quality, an experiment was conducted at the Seed Laboratory, Department of Agronomy, Bangladesh Agricultural University Mymensingh in 2008 and 2009. In 2008, soybean seed has 96% initial germination and in 2009 seed having 98% initial germination was stored at 8% and 12% initial moisture levels in two types of storage containers viz., cloth bag and polythene bag (0.06mm thickness). The final seed moisture content, germination percentage, germination index, and seedling dry matter of the seed under different treatments were measured at 60,120, and 180 days after storage (DAS). The experiment was arranged in a completely randomized design with three replications. In 2008, highest germination percentage (89.33%) of soybean seed was retained at 180 days after storage (DAS) for those stored at 8% initial seed moisture content (SMC) in polythene bag at 50% relative humidity. Germination index and seedling dry matter decreased with increased initial seed moisture content irrespective of storage containers used. In 2009, highest germination percentage (92.67%) of soybean seed was retained at 180 DAS for those stored in polythene bag at 8% initial SMC at 50% of the relative humidity. Those stored in cloth bag at 12% SMC showed rapid germination loss and the value went down to 0.00 in both the years. Vigour index and seedling dry matter decreased with increased initial seed moisture content irrespective of storage containers used.

Keywords: Soybean, Humidity, Seed moisture, Viability, Vigour.

Location: Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Postharvest and Agro-processing

Commodity: Soybean

Objectives:

Therefore, an experiment was undertaken to find out the effect of relative humidity, initial seed moisture content and storage container on soybean seed quality.

Methodology:

The experiment was conducted at the Seed Laboratory of the Department of Agronomy, Bangladesh Agricultural University, Mymensingh during May to November 2008 and 2009. The seeds were stored at four different levels of relative humidity viz., 50, 60, 70, and 80% in polythene bag and cloth bag with 8.0 and 12.0% initial moisture content. The treatments were arranged in Completely Randomized Design with three replications. The soybean variety Shohag (Pb 1) were collected from Regional Agricultural Research Station, BARI, Jamalpur. Relative humidity in the storage (cleaned and sterilized glass jar of 28.0 cm diameter and 35.6 cm height) were created using table salt (NaCl₂) and water at different ratios. The salt (g) and water (ml) in the ratio 200:0, 100:0, 50:50, and 25:75 to create 50, 60, 70, and 80 % RH were taken in the glass jar. Five hundred grams of seeds were taken in each of cloth or polythene bags. Samples were taken at 60 days interval for 180 days. The relative humidity of storage container was measured with the hygrow meter. The moisture content, germination percentages, germination index and seedling dry matter were determined. Seed moisture content was measured using high constant temperature oven dry method following ISTA rules (2003). About 5-8 g of soybean seeds were placed in aluminium dish and dried in oven at 130⁰C for 2 hours (until constant weight achieved) Data analysis was done statistically following the analysis of variance (ANOVA) technique and the means were compared by Duncan’s Multiple Range Test (Gomez and Gomez, 1984).

Source of Information: Bangladesh J. Agril. Res. 39(3): 461-469, September 2014 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the result of the study, it was concluded that soybean seed could be stored with above 80% germination for at least six months under a range of relative humidity (50 to 60%) if stored in polythene bag after drying to 8% initial seed moisture content.

Knowledge Management: Journal