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Root Initiation in Mukhikachu (colocasia Esculenta)as Influenced by Iaa and Naa

M. K. R. BHUIYAN
Scientific Officer
Citrus Research Station, BARI, Jaintapur, Sylhet

M. J. HOSSAIN
Principal Scientific Officer
Research Wing, BARI, Joydebpur Gazipur

M. S. RAHMAN
Scientific Officer
Citrus Research Station, BARI, Jaintapur, Sylhet

S. M. L. RAHMAN
Senior Scientific Officer
Citrus Research Station, BARI, Jaintapur, Sylhet

M. A. SATTAR
Senior Scientific Officer
HRC, BARI, Joydebpur, Gazipur, Bangladesh

Abstract/ Executive Summary:

In vitro root initiation in Mukhikachu (Colocasia esculenta var. globulifera) was assessed in a factorial experiment using three levels of IAA (0.5, 1.0, and 2.0 mg/l), three levels of NAA (0.5, 1.0, and 2.0 mg/l) and control. Fifty percent intact shoots were used as usual, which was named as normal cut explant and the rest 50 % shoots were cut slantly to expose fresh surface i.e., cambium zone and named as slant cut explant. Low levels of IAA (0.5mg/l) initiated the roots earliest (≈14 DAC) and gave the highest percentage of root (49.71). This treatment also gave the maximum roots/culture (3.63). Root initiation was higher (61.33 %) with slant cut when cultured on a medium containing 0.5 mg/l IAA. The cultures with slant cut end also produced more number of roots and longest roots whereas, the highest root initiation (45.05 %) was given by the treatment 1.0 mg/l NAA, but 2.0 mg/I NAA gave lower percentage of roots (39.89).The maximum number of roots/culture was also obtained by 1.0 mg/l NAA. Slant cut explant performed better regarding root initiation (%), number of roots/culture and length of roots. In this experiment, slant cut explant performed better than that of normal cut and either IAA (0.05 mg/l) or NAA (1.0 mg/l) might be used for root initiation in Mukhikachu.

Keywords: Root initiation, Colocasia, IAA, NAA

Location: Tuber Crops Research Centre (TCRC), BARI, Joydebpur, Gazipur

Start Date: 00-07-2008

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Aroids

Objectives:

This investigation was undertaken to develop efficient protocols for organogenesis of taro,

Methodology:

The experiment was conducted at the Tissue Culture Laboratory of the Tuber Crops Research Centre (TCRC), BARI, Joydebpur, Gazipur in July 2008. The excised individual multiple shoot of Mukhikachu cultivar “Bilashi” (Colocasia esculenta var, globulifera) was used as explant of this experiment. Fifty percent detached shoots were used as it is and the rest 50 % shoots were given slant cut to expose fresh surface i.e., cambium zone. Those were then cultured in the Murashige and Skoog culture medium. Basic salts of Murashige and Skoog (MS 1962) culture media were used. In order to regeneration of adventitious roots, a two-factor experiment was set up. Factor a was two types of explants: slant cut and normal cut explant. Factor b was seven levels of rooting hormone: three levels of IAA (0.5, 1.0, and 2.0 mg/l), three levels of NAA (0.5, 1.0, and 2.0 mg/I) and control (at 0 level of IAA and NAA). Total number of treatments was 14 including control. The culture media were prepared as per treatments and agar @ 0.6% was used to solidify the media. The pH of media was adjusted to 5.8 prior to autoclaving. The cultures were incubated under 3.0 k lux photo-intensity for I6h daily and at a temperature of 22 ± 2°C. The experiment was set up in a completely randomized design with three replications. Five tubes of each treatment considered as a replication. Data were analyzed statistically and mean differences were evaluated by DMRT.

Source of Information: Bangladesh J. Agril. Res. 36(3) : 487-494, September 2011, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Low levels of IAA (0.5mg/l) initiated the roots earliest (≈14 DAC) and gave the highest percentage of root (49.71). This treatment also gave the maximum roots/culture (3.63). Root initiation was higher (61.33 %) with slant cut when cultured on a medium containing 0.5 mg/l IAA. The cultures with slant cut end also produced more number of roots and longest roots whereas, the highest root initiation (45.05 %) was given by the treatment 1.0 mg/l NAA, but 2.0 mg/I NAA gave lower percentage of roots (39.89).The maximum number of roots/culture was also obtained by 1.0 mg/l NAA. Slant cut explant performed better regarding root initiation (%), number of roots/culture and length of roots. In this experiment, slant cut explant performed better than that of normal cut and either IAA (0.05 mg/l) or NAA (1.0 mg/l) might be used for root initiation in Mukhikachu.

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