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Antibiogram of Single, Double and Triple Chain Aroyl Hydrazine Against Some Gram Positive and Gram Negative Bacteria.

Parvin R
Sylhet Govt. College

Absar M N
Department of Chemistry, Jahangirnagar University

Ershaduzzaman M
Bangladesh Livestock Research Institute, Savar

Mahbub-E-Elahi A T M
Department of Microbiology& Hygiene, Sylhet Agricultural University

Shil A
Department of Botany, Jahangirnagar University, Bangladesh.

Abstract/ Executive Summary:

The antibacterial sensitivity of some single, double and triple chain aroylhydrazine against gram positive and gram negative bacteria were performed by disc diffusion method. Most of the compounds showed appreciable antibacterial activity against different gram positive and gram negative bacteria. The single chain hydrazines are more active then double chain and triple chain hydrazine. Among the single chain aroylhydrazines studied only 4-n-hexyloxy benzoyl hydrazine is the most active. The significant activity of 4-n-hexyloxybenzoyl hydrazine and heptyloxybenzoyl hydrazine against gram positive and gram negative bacteria may be (formation of inhibition zone 8 to 22 mm with most of the test bacteria) due to their liophilicity of the bacterial cell membrane. Anti-microbial activity decreases as the number of carbon of single chain hydrazine increases (C6 > C7 > C8 > C9 > C10 single chain hydrazine). Double chain hydrazines (3, 5 or 3, 4) are more active than triple chain hydrazines (3, 5 > 3, 4 > 3, 4, 5 hydrazine). The antibacterial activities of hydrazines are being decreased as their increasing number of side chain.

Keywords: Antibacterial sensitivity, Aroyl hydrazine.

Location:

Start Date:

End Date:

Program Area: Pest Management

Commodity: Diseases

Objectives:

The present paper report the study of the antibacterial activity of single chain, double chain and triple chain aroyl hydrazine. Pure culture strain of ten gram positive and gram negative bacteria were used in this study

Methodology:

The susceptibility of the microorganisms to antimicrobial agents may be measured in vitro by utilizing agar diffusion technique provided that all the procedural details are carefully controlled. Dried dispersed batches of 100 discs in screw capped bottles were sterilized in autoclave. In an aseptic condition the test material hydrazine was allowed to absorb into the filter paper disc (400 μ gm/disk) and left over night for complete removal of solvent (dichloromethane). Anti-microbial assay procedure The test organisms from the pure culture were transferred to the slant with the help of an inoculation loop in an aseptic condition. After inoculation the slant was subjected to incubation at 37⁰C for 12-24 hours to provide sufficient time and temperature for the growth of the test organisms. Cultures were used within two or three days. The test organisms from slant were transferred to nutrient broth and incubate at 37⁰C for 24 hours for sufficient growth. After incubation turbidity was observed in the test tube, it was ready to use as test organisms. On the other hand nutrient agar was poured into Petri dishes on a level horizontal surface so as to give a uniform depth of approximately 4mm. After the medium had been allowed to cool to room temperature it was stored in a refrigerator. Synthesis of hydrazine: All chemicals were obtained from ACORS chemicals and were used without further purification. Except ethanol and 1-butanol distilled over anhydrous calcium oxide and dichloromethane was distilled over anhydrous P₂O₅. Infrared spectra in the range 4000-400 cm-1 were recorded from an intimated mixture of the compounds and KBr using a JASCO FT-IR-460+TLUS spectrometer. Synthesis of alkoxybenzoates: (1a-23a Scheme-1) All the single, double and triple chain alkyloxybenzoates were synthesized by using a general procedure, the synthetic routes shown in scheme-1, a representative detail for ethyl-4-nhexyloxybenzoate is given below: Ethyl-4-hydroxybenzoate (1 mole) and 1 bromoalkane (1 X 1.2 mole ratio) mix together with anhydrous potassium carbonate (1×1.5 mole ratio), made a salary and was irradiated with microwave radiation at medium law power (384w) for 3.5 minutes (Scheme-1). Extraction of the reaction product with dichloromethane and hexane (2:1v/v) followed by chromatographic purification over silica gel gave ethyl-4-n-hexyloxy-benzoate.

Source of Information: International Journal of Natural Sciences (2011), 1(1):17-21

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Achievement/Findings:

Single chain hydrazines are more active than double and triple chain hydrazines. Anti-microbial activity decreases as the number of carbon of single and double chain hydrazine increases, (C6 > C7 > C8 > C9 > C10 single and double chain hydrazine.) Double chain hydrazines (3, 5 or 3, 4) are more active than triple chain hydrazines, (3, 5 > 3, 4 > 3, 4, 5 hydrazine). The poor activity of other hydrazines (3, 4, 5, some 3, 5, some 3, 4, and single chain) may be due to the hydrophobic properties of these hydrazines which may inhibits permeation through the lipoid layers of microorganism membranes. The significant activity of hexyloxybenzoyl hydrazine and heptyloxybenzoyl hydrazine against gram positive and gram negative bacteria may be (formation of inhibition zone 8 to 22 mm with most of the test bacteria) due to their liophilicity of the bacterial cell membrane.

Knowledge Management: Journal