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Screening of Chickpea Genotypes Against Salinity Stress

MD. SHAHEENUZZAMN
Scientific Officer,
Agronomy Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur

Abstract/ Executive Summary:

The experiment was conducted during the period from December 13, 2010 to May 13, 2011 at the Crop Physiology Lab, Agronomy Division, Bangladesh Agricultural Research Institute (BARI). In Hoagland culture solution, 70 (Seventy) genotypes of chickpea were tested during germination and seedling stage at 0, 5, 10 and 15 dS/m salinity levels. Distilled water (0 dS/m) was used as a control. Germination percentage (GP), relative germination percentage (RGP), germination rate (GR), relative germination rate (RGR), root length (RL), relative root length (RRL), shoot length (SL), relative shoot length (RSL), vigor index, total dry matter (TDM) and relative total dry matter (RTDM) were found to be affected by salinity. Genotypes BD-6061, BD-6066 BD-6071, BD- 6060, BD-6067 and BD 6078 performed better at 10 dS/m and survived up to 15 days after germination as evaluated on the basis of germination percentage (GP), relative germination percentage (RGP), total dry matter (TDM) and relative total dry matter (RTDM). These genotypes could be selected for further investigation under pot culture and field evaluation in the saline area.

Keywords: Chickpea, Genotype, Salinity level, Hongland and Pot culture

Location: Agronomy Division, Bangladesh Agricultural Research Institute (BARI, Gazipur)

Start Date: 13-12-2010

End Date: 13-05-2011

Program Area: Variety and Species

Commodity: Chickpea

Objectives:

The objective of this experiment was to evaluate genotypes of Chickpea for their salt tolerance.

Methodology:

The experiment was conducted during the period from December 13, 2010 to May 13, 2011 at the Crop Physiology Lab, Agronomy Division, Bangladesh Agricultural Research Institute (BARI). Seeds of seventy genotypes of chickpea were collected from Plant Genetics Resource Center (PGRC), BARI, Joydebpur, Gazipur and subjected to attain different salinity levels. The experiment assessed the germination and seedling growth of chickpea genotypes at different NaCl salinity levels. The NaCl concentrations used were 0 (control), 5, 10, and 15 dS/m and 70 genotypes were used as treatment variables. The salt solution was prepared by calculating amount of normal NaCl in distilled water. Hongland solution was used as nutrient media with the salt solution. The PH of Hongland solution was maintained 6-7. Plastic pots were used in the experiment with a diameter of 10 cm and arranged in a completely randomized design with three replications. Each pot was supplied with 500 ml of the respective treatment solution. Seeds were sown on the plastic pots having bolting paper. The germination count was started after 72 hours of sowing and continued till the 13th day. A seed was considered to have germinated when both the plumule and the radicle emerged > 0.5cm. After 15 days, the shoot and the root length of ten randomly selected seedlings from each replicate were measured following a draftsman ruler (Azhar and McNeilly, 1987). Data analysis was carried out by SAS package where two way analysis of variance (ANOVA) and correlation analysis were employed. Prior to data analysis, shoot-to-root ratio (SRR) was log transformed. At 15 dS/m, almost all genotypes of chickpea were collapsed after germination. Consequently, the incomplete data obtained from these salinity levels had been excluded from the data. Analysis of germination percentage (GP), relative germination percentage (RGP), germination rate (GR), relative germination rate (RGR), root length (RL), relative root length (RRL), shoot length (SL), relative shoot length (RSL), total dry matter (TDM) and relative total dry matter (RTDM) were measured.

Source of Information: ISSN 0258-7122 Bangladesh J. Agril. Res. 39(4): 605-619, December 2014

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The salt tolerant genotypes found in the diverse germplasm of chickpea could have considerable economic value in increasing yield on saline areas with moderate salinity, provided the genotypes are still tolerant when adult and also have adaptability to other factor counted in salt affected soils. The genotypes BD-6061, BD-6066 BD-6071, BD-6060, BD-6067 and BD 6078 should be tested in pot experiment for confirmation in the next season.

Knowledge Management: Journal