Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Seroprevalence of Mycoplasma Gallisepticum Infection in Chicken in the Greater Rajshahi District of Bangladesh

K. M. M. Hossain
Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Bangladesh.

M. Y. Ali
Upazila Veterinary Hospital, Paba, Rajshahi, Bangladesh.

M. I. Haque
Zonal Poultry Farm, Rajshahi, Bangladesh.

Abstract/ Executive Summary:

A serological investigation of Mycoplasma gallisepticum (MG) infection in chicken was conducted in the greater Rajshahi district of Bangladesh on 115 flocks during the period from July 2006 to June 2007. A total of 575 sera samples were collected and tested by serum plate agglutination (SPA) test using Mycoplasma gallisepticum antigen (Nobilis®MG, Intervet International B.V. Boxmeer-Holland) to determine specific antibodies in different flocks. The overall seroprevalence of MG infection in different flocks was recorded as 55.13%. Seroprevalence of MG infection was found significantly (p<0.05) higher during winter season (61.48%) than in summer (47.74%). Again this was recorded in different age groups, with significantly (p<0.01) higher occurrence in young (72.72%) compared to adult (44.00%). On the other hand, the seroprevalence of MG infection was found little (p>0.05) higher in large flocks (62.86%) in comparison to small flocks (52.00%). It has been found that MG infection is still an important disease problem in chickens in Bangladesh.

Keywords: Seroprevalence, Mycoplasma gallisepticum, Chicken, Serum plate agglutination

Location: Lab Avian laboratory, Rajshahi and Department of Animal Husbandry and Veterinary Science, University of Rajshahi, Bangladesh.

Start Date: 00-07-2006

End Date: 00-06-2007

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To determine the seroprevalence of mycoplasmosis in chickens to undertake an effective control measure.

Methodology:

A total of one hundred and fifteen layer chicken (18-71 week age) flocks were selected in different upazilas of the greater Rajshahi district. From each flock five chickens (a total of 575 adult chickens) were randomly selected for blood collection. 2 ml of blood was collected aseptically from wing vein of each bird and then sera were separated and stored at -21°C until use for serum plate agglutination test. The MG antigen used here in this study was purchased from the Intervet International B.V. Boxmeer-Holland (the antigen was a suspension of killed and stained S-6 Adler strain of Mycoplasma gallisepticum organism). The SPA test was conducted according to the instructions of antigen manufacturer (Intervet International B. V. Boxmeer-Holland). One drop (0.05 ml) of test serum was mixed with one drop (0.05 ml) of antigen on a clean glass plate at room temperature (25⁰C). The glass plate was illuminated from below as to facilitate observing the reaction, avoiding excessive heat from the light source. Positive reaction was characterized by the formation of definite clumps within 2 minutes after mixing the test sera with the antigen. The clumps usually started and concentrated at the periphery of the mixture. Negative reaction was indicated by absence of agglutination reaction. Care was taken so that the natural granulation of the antigen (due to the presence of whole cells) was not taken as a positive reaction. The strength of the agglutination reaction was measured according to the following scheme: − = No clumps, no background clearing; + = Small clumps, no background clearing; ++ = Medium sized clumps, almost complete background clearing; +++ = Large clumps, almost complete background clearing; ++++ = Very large clumps, mostly in the periphery; complete background clearing. The seroprevalence of MG infection with season of year, age of birds and flock size were compared by means of the Chi-square test. A significant level of 5% was used.

Source of Information: Bangl. J. Vet. Med. (2007). 5 (1 & 2): 09–14

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among 317 seropositive chickens, 190 were found during winter (October to March) and 127 were found during summer (April to September) season. The seropositivity in chickens was 61.49% in winter compared to 47.74% in summer which supports the finding. Similar report was demonstrated who reported 62.44% prevalence in winter in comparison to 53.10% in summer. This finding also supports the report who recorded highest prevalence of MG infection in winter (61.45%) than in rainy (57.47%) season. This seasonal variation in prevalence of MG infection might be due to the influence of cold weather. According to the age, the highest prevalence of MG infection was 72.72% in 18-25 weeks age group whereas lowest prevalence was 44.00% in 66 weeks and above age group. Similar report was demonstrated who reported highest MG infection (71.42%) at 18 weeks of age and lowest (55.17%) at 63 weeks of age. This finding also supports the report who recorded 73.80% MG infection at 20 weeks of age in comparison to 45.16% at 55 weeks of age. Similar report was demonstrated who reported the prevalence of MG infection significantly decreased with the increase of age. Highest infection in the young chickens is due to the vertical transmission of the organisms. Serological investigation in the greater Rajshahi district showed the highest infection rate (62.86%) in large scale flocks (≥5001 birds) in comparison (52.00%) to small (500-1000 birds) flocks. Similar report was demonstrated who recorded 36% MG infection in a flock containing 300 chickens in comparison to 33% in a flock containing 250 chickens. Highest infection rate in large scale flocks probably due to faulty in management and bio-security.

Knowledge Management: Journal