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Effect of Water Stress on Stomatal Characters of Twenty One Near Isogenic Lines of Wheat (triticum Aestivum L.)

A. M. S. ALAM
Professor
Department of Botany, University of Rajshahi.

G. KABIR
Professor
Department of Botany, University of Rajshahi.

M. M. UD-DEEN
Associate Professor
Department of Crop Science & Technology, University of Rajshahi

E. HOQUE
Assistant Professor
Department of Botany, Godagari Degree College, Rajshahi, Bangladesh.

Abstract/ Executive Summary:

The present study was carried out to determine the effect of water stress on stomatal characters of flag leaf of wheat (Triticum aestivum L.) where five different irrigation regimes were considered as environments. Stomatal opening frequencies were significantly decreased by water stress in both the surfaces of the flag leaf in both irrigated and rainfed conditions in all the near isogenic lines of wheat. In rainfed condition, it was lower than irrigated condition. Similarly stomatal index also significantly differed in different irrigation treatments at three different positions in both adaxial and abaxial surface but under rainfed condition, it was lower than the irrigated condition in all the genotypes. The pore lengths of flag leaf in both adaxial and abaxial surfaces were found similar in size. It also varied among the different irrigations, but the variations were not remarkable. In rainfed condition, the pore lengths of different genotypes showed lower values than irrigated condition. Effects of water stress on stomatal pore breadths in both the surfaces were significantly decreased in rainfed condition. But in different irrigation conditions, stomatal pore breadth were non-significant among the genotypes. The effect of water stress on different stomatal characters in both surface of leaf in different lines of wheat were decreased.

Keywords: Water stress, Stomatal characters, Wheat

Location: Experimentation field of Rajshahi University

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Wheat

Objectives:

To determine the water stress sensivity for area and stomatal behaviour of flag leaves of twenty one Near Isogenic Lines (NILs) of wheat (Triticum aestivum L.) at various growth stages under different schedule of irrigations and rainfed condition.

Methodology:

Twenty one Near Isogenic Lines (NILs) of wheat (Triticum aestivum L.) obtained from different crosses between four Bangladeshi varieties (Aghrani, Akbar. Ananda, and Kanchan) and two dwarf selected lines (FM-132 and FM-139) of wheat were used as plant materials. The seeds of these lines were procured from the Cytogenetics Laboratory, Department of Botany, University of Rajshahi, Bangladesh. The present investigations were conducted in the experimentation field of Rajshahi University. The experimentation fields were prepared after repeated ploughings. The soil was measured properly. Uniform and saturated intercultural operations were done as and when necessary for all traits to raise the better crop. The Near Isogenic Lines of wheat were raised in split plot with three replications and evaluated at five irrigation (I) conditions, I₃ (irrigation was given at the root crowning stage, I₂ (I₂, + at the tillering stage), I₃ (I₃ + at the booting stage), I₃ (I₃ + at the heading stage), and I0 (rainfed condition) treated as control. Transparent quick fix adhesives were spread on both the adaxial and abaxial surfaces of flag leaf. Allowed to dry for 30 minutes and then thin film of quick fix were taken out with the help of a fine forceps. The numbers of stomata from 30 random focuses from each surface of leaves were counted under a compound microscope and subsequently, the values were converted and expressed as number per mm² of leaf area. The pore length and pore breadth of stomata of leaves were measured with the help of occulometer in a compound microscope using the values were converted into micron (µm) and the stomatal index of both ahaxial and adaxial surfaces of flag leaf of different genotypes were calculated and the data were analyzed statistically using conventional method.

Source of Information: Bangladesh J. Agril. Res. 36(1) : 173-181, March 2011, ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Stomatal opening frequencies were significantly decreased by water stress in both the surfaces of the flag leaf in both irrigated and rainfed conditions in all the near isogenic lines of wheat. In rainfed condition, it was lower than irrigated condition. Similarly stomatal index also significantly differed in different irrigation treatments at three different positions in both adaxial and abaxial surface but under rainfed condition, it was lower than the irrigated condition in all the genotypes. The pore lengths of flag leaf in both adaxial and abaxial surfaces were found similar in size. It also varied among the different irrigations, but the variations were not remarkable. In rainfed condition, the pore lengths of different genotypes showed lower values than irrigated condition. Effects of water stress on stomatal pore breadths in both the surfaces were significantly decreased in rainfed condition. But in different irrigation conditions, stomatal pore breadth were non-significant among the genotypes. The effect of water stress on different stomatal characters in both surface of leaf in different lines of wheat were decreased.

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